KIT, also known as c-KIT, SCFR (stem cell factor receptor), PBT (Piebald trait protein), and CD117, is a single pass type I transmembrane protein belonging to the receptor tyrosine kinase (RTK) family and the CSF-1/PDGF receptor subfamily.  KIT is a cellular homolog of the feline  sarcoma virus protein, v-Kit. Kit contains 5 immunoglobulin (Ig)-like C2-type domains in the extracellular domain (ECD) and 1 split tyrosine kinase domain in the cytoplasmic region. Alternative splicing of KIT generates a potentially secreted isoform corresponding to the first 4 Ig-like domains.  KIT may act as cell-surface receptor for the cytokine KITLG/SCF and play an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF, KIT can activate PI3K and AKT1 signaling pathways. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases, MAPK1/ERK2 and/or MAPK3/ERK1. KIT promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Mutations in the KIT gene are associated with gastrointestinal stromal tumors (GIST), mast cell disease, acute myelogenous leukemia (AML), and piebaldism. KIT protects  vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction.

KIT, also known as c-KIT, SCFR (stem cell factor receptor), PBT (Piebald trait protein), and CD117, is a single pass type I transmembrane protein belonging to the receptor tyrosine kinase (RTK) family and the CSF-1/PDGF receptor subfamily.  KIT is a cellular homolog of the feline  sarcoma virus protein, v-Kit. Kit contains 5 immunoglobulin (Ig)-like C2-type domains in the extracellular domain (ECD) and 1 split tyrosine kinase domain in the cytoplasmic region. Alternative splicing of KIT generates a potentially secreted isoform corresponding to the first 4 Ig-like domains.  KIT may act as cell-surface receptor for the cytokine KITLG/SCF and play an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF, KIT can activate PI3K and AKT1 signaling pathways. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases, MAPK1/ERK2 and/or MAPK3/ERK1. KIT promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Mutations in the KIT gene are associated with gastrointestinal stromal tumors (GIST), mast cell disease, acute myelogenous leukemia (AML), and piebaldism. KIT protects  vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction.

 

Product Details

 

Gene Symbol: KIT; PBT; SCFR; C-Kit; CD117

 

NCBI Gene ID: 3815

 

Uniprot Entry: P10721

 

Construct Details: The recombinant human KIT-Fc protein is expressed as a 737-amino acid protein consisting of Gln26 - Pro524 region of KIT with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.   

  

Source: Human cells stably expressing human KIT-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QPSVSPGEPS PPSIHPGKSD LIVRVGDEIR LLCTDPGFVK WTFEILDETN ENKQNEWITE 

        70         80         90        100        110        120 

KAEATNTGKY TCTNKHGLSN SIYVFVRDPA KLFLVDRSLY GKEDNDTLVR CPLTDPEVTN 

       130        140        150        160        170        180 

YSLKGCQGKP LPKDLRFIPD PKAGIMIKSV KRAYHRLCLH CSVDQEGKSV LSEKFILKVR 

       190        200        210        220        230        240 

PAFKAVPVVS VSKASYLLRE GEEFTVTCTI KDVSSSVYST WKRENSQTKL QEKYNSWHHG 

       250        260        270        280        290        300 

DFNYERQATL TISSARVNDS GVFMCYANNT FGSANVTTTL EVVDKGFINI FPMINTTVFV 

       310        320        330        340        350        360 

NDGENVDLIV EYEAFPKPEH QQWIYMNRTF TDKWEDYPKS ENESNIRYVS ELHLTRLKGT 

       370        380        390        400        410        420 

EGGTYTFLVS NSDVNAAIAF NVYVNTKPEI LTYDRLVNGM LQCVAAGFPE PTIDWYFCPG 

       430        440        450        460        470        480 

TEQRCSASVL PVDVQTLNSS GPPFGKLVVQ SSIDSSAFKH NGTVECKAYN DVGKTSAYFN 

       490        500        510        520        530        540 

FAFKGNNKEQ IHPHTLFTPS TTENLYFQGS TGTHTCPPCP APELLGGPSV FLFPPKPKDT 

       550        560        570        580        590        600 

LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 

       610        620        630        640        650        660 

QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK NQVSLTCLVK 

       670        680        690        700        710        720 

GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 

       730 

ALHNHYTQKS LSLSPGK 

 

  

M.W.: Calculated molecular mass (kDa): 82.8 Estimated by SDS-PAGE under reducing condition (kDa): 90-100.

 

Calculated PI: 6.27

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 106325

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds its ligand KITL/SCF (SKU#FCL2346 and #FCL2384) and neutralizes its mediated signaling activity.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. EMBO J. 6:3341-3351(1987) 

2. Oncogene 7:2207-2217(1992)

3. J. Biol. Chem. 269:21793-21802(1994)

4. Cell. Mol. Life Sci. 61:2535-2548(2004)

5. Clin. Cancer Res. 14:1926-1930(2008)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2650-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.