CD6, also known as TP120, is a single-pass type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 contains three SRCR domains in the extracellular region. CD6 is a cell surface glycoprotein expressed primarily on T cells and thus a T-cell differentiation antigen. CD6 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some neuronal cells in the brain.  CD6 may function as both a co-stimulatory molecule in T cell activation and as an adhesion receptor.  As a cell adhesion molecule, CD6 mediates cell-cell contacts and regulates T cell responses via its interaction with the ligand ALCAM (activated leukocyte cell adhesion molecule) or CD166. CD6 partially associates with the TCR/CD3 complex and colocalizes with it at the center of the mature immunological synapse on T lymphocytes. CD6/ALCAM interactions are thought to play a role in thymocyte development.  CD6 may also contribute to signaling cascades triggered by activation of the TCR/CD3 complex and the overall functional avidity of selection. CD6 also functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. CD6 binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. CD6 thus mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS. CD6 may be associated with susceptibility to multiple sclerosis

CD6, also known as TP120, is a single-pass type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 contains three SRCR domains in the extracellular region. CD6 is a cell surface glycoprotein expressed primarily on T cells and thus a T-cell differentiation antigen. CD6 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some neuronal cells in the brain.  CD6 may function as both a co-stimulatory molecule in T cell activation and as an adhesion receptor.  As a cell adhesion molecule, CD6 mediates cell-cell contacts and regulates T cell responses via its interaction with the ligand ALCAM (activated leukocyte cell adhesion molecule) or CD166. CD6 partially associates with the TCR/CD3 complex and colocalizes with it at the center of the mature immunological synapse on T lymphocytes. CD6/ALCAM interactions are thought to play a role in thymocyte development.  CD6 may also contribute to signaling cascades triggered by activation of the TCR/CD3 complex and the overall functional avidity of selection. CD6 also functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. CD6 binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. CD6 thus mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS. CD6 may be associated with susceptibility to multiple sclerosis

 

Product Details

 

Gene Symbol: CD6; TP120; T12

 

NCBI Gene ID: 923

 

Uniprot Entry: P30203

 

Construct Details: The recombinant human CD6-Fc is expressed as a 619-amino acid protein consisting of His18 - Glu398 region of CD6 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

  

Source: Human cells stably expressing human CD6-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

HPSPAPPDQL NTSSAESELW EPGERLPVRL TNGSSSCSGT VEVRLEASWE PACGALWDSR 

        70         80         90        100        110        120 

AAEAVCRALG CGGAEAASQL APPTPELPPP PAAGNTSVAA NATLAGAPAL LCSGAEWRLC 

       130        140        150        160        170        180 

EVVEHACRSD GRRARVTCAE NRALRLVDGG GACAGRVEML EHGEWGSVCD DTWDLEDAHV 

       190        200        210        220        230        240 

VCRQLGCGWA VQALPGLHFT PGRGPIHRDQ VNCSGAEAYL WDCPGLPGQH YCGHKEDAGA 

       250        260        270        280        290        300 

VCSEHQSWRL TGGADRCEGQ VEVHFRGVWN TVCDSEWYPS EAKVLCQSLG CGTAVERPKG 

       310        320        330        340        350        360 

LPHSLSGRMY YSCNGEELTL SNCSWRFNNS NLCSQSLAAR VLCSASRSLH NLSTPEVPAS  

       370        380        390        400        410        420 

VQTVTIESSV TVKIENKESR ESTTENLYFQ GSTGTHTCPP CPAPELLGGP SVFLFPPKPK 

       430        440        450        460        470        480 

DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV  

       490        500        510        520        530        540 

LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL 

       550        560        570        580        590        600 

VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM 

       610 

HEALHNHYTQ KSLSLSPGK 

  

M.W.: Calculated molecular mass (kDa): 67.3; Estimated by SDS-PAGE under reducing condition (kDa): 90-100.

 

Calculated PI: 5.62

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 112225

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand ALCAM/CD166 and neutralizes ALCAM/CD166-mediated signaling activity.

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Immunol. 143:2439 (1989)

2. J. Exp. Med. 174:949 (1991)

3. Eur. J. Immunol. 25:2765 (1995)

4. J. Immunol. 158:1149 (1997)

5. J. Immunol. 178:4351 (2007)

6. Proc. Natl. Acad. Sci. 104:11724 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2579-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.