B-cell differentiation antigen CD72, also known as Lyb2, is a single pass type II transmembrane glycoprotein that plays an important role in adaptive immunity. CD72 contains a 95 amino acid (aa) cytoplasmic domain with 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain (ECD) with a coiled-coil domain and a C-type lectin domain. CD72 is expressed on B lineage cells including Pre-B-cells and B-cells but not terminally differentiated plasma cells, NK cells, monocytes, dendritic cells, and mast cells. CD5 exists as a disulfide-linked homodimer. It interacts with CD5 and with CD100/Semaphorin 4D.  CD5 also associates with CD79a in the B cell antigen receptor (BCR) complex following antigen stimulation.  CD5 inhibits BCR signaling through interactions with the phosphatase PTPN6/SHP-1. CD72 ligation with antibodies or with CD100 induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP-1, leading to B cell proliferation. CD100 is expressed on activated NK cells and the adhesive interaction between CD100+ NK cells and CD72+ cells leads to an enhanced killing and IFN­γ secretion.  Both CD72 and CD100 are required for the maintenance of B cell anergy and tolerance.  Mice that lack either CD72 or CD100 develop autoimmunity.  CD72 may also induce positive signaling in B cells independent of the BCR signaling. 

B-cell differentiation antigen CD72, also known as Lyb2, is a single pass type II transmembrane glycoprotein that plays an important role in adaptive immunity. CD72 contains a 95 amino acid (aa) cytoplasmic domain with 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain (ECD) with a coiled-coil domain and a C-type lectin domain. CD72 is expressed on B lineage cells including Pre-B-cells and B-cells but not terminally differentiated plasma cells, NK cells, monocytes, dendritic cells, and mast cells. CD5 exists as a disulfide-linked homodimer. It interacts with CD5 and with CD100/Semaphorin 4D.  CD5 also associates with CD79a in the B cell antigen receptor (BCR) complex following antigen stimulation.  CD5 inhibits BCR signaling through interactions with the phosphatase PTPN6/SHP-1. CD72 ligation with antibodies or with CD100 induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP-1, leading to B cell proliferation. CD100 is expressed on activated NK cells and the adhesive interaction between CD100+ NK cells and CD72+ cells leads to an enhanced killing and IFN­γ secretion.  Both CD72 and CD100 are required for the maintenance of B cell anergy and tolerance.  Mice that lack either CD72 or CD100 develop autoimmunity.  CD72 may also induce positive signaling in B cells independent of the BCR signaling. 

 

Product Details

 

Gene Symbol: CD72; LYB2; CD72b; Lyb-2

 

NCBI Gene ID: 971

 

Uniprot Entry: P21854

 

Construct Details: The recombinant human CD72-Fc protein is expressed as a 468-amino acid protein consisting of Gln120 - Glu359 region of CD72 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.   

 

Source: Human cells stably expressing human CD72-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QVSQQLQQTN RVLEVTNSSL RQQLRLKITQ LGQSAEDLQG SRRELAQSQE ALQVEQRAHQ 

        70         80         90        100        110        120 

AAEGQLQACQ ADRQKTKETL QSEEQQRRAL EQKLSNMENR LKPFFTCGSA DTCCPSGWIM 

       130        140        150        160        170        180 

HQKSCFYISL TSKNWQESQK QCETLSSKLA TFSEIYPQSH SYYFLNSLLP NGGSGNSYWT 

       190        200        210        220        230        240 

GLSSNKDWKL TDDTQRTRTY AQSSKCNKVH KTWSWWTLES ESCRSSLPYI CEMTAFRFPD  

       250        260        270        280        290        300 

STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV 

       310        320        330        340        350        360 

DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA 

       370        380        390        400        410        420 

KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD 

       430        440        450        460 

SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK 

 

  

M.W.: Calculated molecular mass (kDa): 53.2 Estimated by SDS-PAGE under reducing condition (kDa): 55-60.

 

Calculated PI: 8.26

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 85215

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to human CD5 (SKU#FCL2664) and CD100 proteins, and induces IFN­γ secretion in human natural killer NK92 lymphoma cells

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 86:1352 (1989)

2. J. Immunol. 144:4870 (1990)

3. Nature 351:662 (1991).

4. J. Immunol. 148:1630 (1992)

5. Immunity 13:621 (2000).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2666-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.