CD26, also known as DPPIV/DPP4 (dipeptidyl peptidase-4) and ADCP2 (adenosine deaminase complexing protein 2), is a single pass type II transmembrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (ECD). The ECD of CD26 is a serine exopeptidase belonging to the S9B family that cleaves Xaa-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones such as glucagon. CD26 plays an important role in many biological and pathological processes. CD2 acts as a T cell-activating molecule and serves as a cofactor for entry of HIV in CD4+ cells. CD26 binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. CD26 exists as a homodimer on the cell surface and the soluble form is also detectable in human serum and other body fluids.   The levels of soluble CD26 may have clinical significance in patients with cancer, liver and kidney diseases, and depression. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels.  Therefore DPP4 inhibitors represent a new pharmacological class of drugs for treating Type 2 diabetes.

CD26, also known as DPPIV/DPP4 (dipeptidyl peptidase-4) and ADCP2 (adenosine deaminase complexing protein 2), is a single pass type II transmembrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (ECD). The ECD of CD26 is a serine exopeptidase belonging to the S9B family that cleaves Xaa-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones such as glucagon. CD26 plays an important role in many biological and pathological processes. CD2 acts as a T cell-activating molecule and serves as a cofactor for entry of HIV in CD4+ cells. CD26 binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. CD26 exists as a homodimer on the cell surface and the soluble form is also detectable in human serum and other body fluids.   The levels of soluble CD26 may have clinical significance in patients with cancer, liver and kidney diseases, and depression. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels.  Therefore DPP4 inhibitors represent a new pharmacological class of drugs for treating Type 2 diabetes.

 

Product Details

 

Gene Symbol: CD26; DPP4; ADABP; ADCP2; DPPIV; TP103

 

NCBI Gene ID: 1803

 

Uniprot Entry: P27487

 

Construct Details: The recombinant human CD26-Fc fusion is expressed as a 965 amino acid protein consisting of Asn29 - Pro766 region of CD26 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human CD26-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

NKGTDDATAD SRKTYTLTDY LKNTYRLKLY SLRWISDHEY LYKQENNILV FNAEYGNSSV 

        70         80         90        100        110        120 

FLENSTFDEF GHSINDYSIS PDGQFILLEY NYVKQWRHSY TASYDIYDLN KRQLITEERI 

       130        140        150        160        170        180 

PNNTQWVTWS PVGHKLAYVW NNDIYVKIEP NLPSYRITWT GKEDIIYNGI TDWVYEEEVF 

       190        200        210        220        230        240 

SAYSALWWSP NGTFLAYAQF NDTEVPLIEY SFYSDESLQY PKTVRVPYPK AGAVNPTVKF 

       250        260        270        280        290        300 

FVVNTDSLSS VTNATSIQIT APASMLIGDH YLCDVTWATQ ERISLQWLRR IQNYSVMDIC 

       310        320        330        340        350        360 

DYDESSGRWN CLVARQHIEM STTGWVGRFR PSEPHFTLDG NSFYKIISNE EGYRHICYFQ 

       370        380        390        400        410        420 

IDKKDCTFIT KGTWEVIGIE ALTSDYLYYI SNEYKGMPGG RNLYKIQLSD YTKVTCLSCE 

       430        440        450        460        470        480 

LNPERCQYYS VSFSKEAKYY QLRCSGPGLP LYTLHSSVND KGLRVLEDNS ALDKMLQNVQ 

       490        500        510        520        530        540 

MPSKKLDFII LNETKFWYQM ILPPHFDKSK KYPLLLDVYA GPCSQKADTV FRLNWATYLA 

       550        560        570        580        590        600 

STENIIVASF DGRGSGYQGD KIMHAINRRL GTFEVEDQIE AARQFSKMGF VDNKRIAIWG 

       610        620        630        640        650        660 

WSYGGYVTSM VLGSGSGVFK CGIAVAPVSR WEYYDSVYTE RYMGLPTPED NLDHYRNSTV 

       670        680        690        700        710        720 

MSRAENFKQV EYLLIHGTAD DNVHFQQSAQ ISKALVDVGV DFQAMWYTDE DHGIASSTAH 

       730        740        750        760        770        780 

QHIYTHMSHF IKQCFSLPDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 

       790        800        810        820        830        840 

VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 

       850        860        870        880        890        900 

VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE 

       910        920        930        940        950        960 

SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS 

 

LSPGK 

  

M.W.: Calculated molecular mass (kDa): 111; Estimated by SDS-PAGE under reducing condition (kDa): ~120

 

Calculated PI: 5.84

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 229975

 

Purity: >92% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.25 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity: Binds human CXCL12 and SDF1b proteins by ELISA and cleaves Xaa-proline dipeptides such as the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC) 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 267:4824-4833(1992)

2. J. Immunol. 149:481-486(1992) 

3. Eur. J. Biochem. 267:5608-5613(2000) 

4. J. Exp. Med. 177:1135-1143(1993)

5. Science 261:466-469(1993)

6. Cancer Res. 66:4652-4661(2006) 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.