CD105 or Endoglin (ENG) is a single-pass type I transmembrane glycoprotein. It is a homodimer and a major glycoprotein of the vascular endothelium. CD105 is involved in the regulation of angiogenesis and plays a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. CD105 is a also component of the transforming growth factor beta receptor (TGFBR)complex and it binds to the beta1 and beta3 peptides with high affinity.  CD105 acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. CD105 is required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. Mutations in the CD105 gene cause hereditary hemorrhagic telangiectasia (HHT1), also known as Osler-Rendu-Weber syndrome 1 (ORW1), an autosomal dominant multisystemic vascular dysplasia. The CD105 gene is also implicated in preeclampsia and several types of cancer.

CD105 or Endoglin (ENG) is a single-pass type I transmembrane glycoprotein. It is a homodimer and a major glycoprotein of the vascular endothelium. CD105 is involved in the regulation of angiogenesis and plays a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. CD105 is a also component of the transforming growth factor beta receptor (TGFBR)complex and it binds to the beta1 and beta3 peptides with high affinity.  CD105 acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. CD105 is required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. Mutations in the CD105 gene cause hereditary hemorrhagic telangiectasia (HHT1), also known as Osler-Rendu-Weber syndrome 1 (ORW1), an autosomal dominant multisystemic vascular dysplasia. The CD105 gene is also implicated in preeclampsia and several types of cancer.

 

Product Details

 

Gene Symbol: CD105; Endoglin; ENG; END; HHT1; ORW1

 

NCBI Gene ID: 2022

 

Uniprot Entry: P17813

 

Construct Details: The recombinant human CD105-Fc fusion is expressed as a 789 amino acid protein consisting of Glu26 - Gly586 region of CD105 and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human CD105-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

ETVHCDLQPV GPERGEVTYT TSQVSKGCVA QAPNAILEVH VLFLEFPTGP SQLELTLQAS 

        70         80         90        100        110        120 

KQNGTWPREV LLVLSVNSSV FLHLQALGIP LHLAYNSSLV TFQEPPGVNT TELPSFPKTQ  

       130        140        150        160        170        180 

ILEWAAERGP ITSAAELNDP QSILLRLGQA QGSLSFCMLE ASQDMGRTLE WRPRTPALVR 

       190        200        210        220        230        240 

GCHLEGVAGH KEAHILRVLP GHSAGPRTVT VKVELSCAPG DLDAVLILQG PPYVSWLIDA 

       250        260        270        280        290        300 

NHNMQIWTTG EYSFKIFPEK NIRGFKLPDT PQGLLGEARM LNASIVASFV ELPLASIVSL 

       310        320        330        340        350        360 

HASSCGGRLQ TSPAPIQTTP PKDTCSPELL MSLIQTKCAD DAMTLVLKKE LVAHLKCTIT 

       370        380        390        400        410        420 

GLTFWDPSCE AEDRGDKFVL RSAYSSCGMQ VSASMISNEA VVNILSSSSP QRKKVHCLNM 

       430        440        450        460        470        480 

DSLSFQLGLY LSPHFLQASN TIEPGQQSFV QVRVSPSVSE FLLQLDSCHL DLGPEGGTVE 

       490        500        510        520        530        540 

LIQGRAAKGN CVSLLSPSPE GDPRFSFLLH FYTVPIPKTG TLSCTVALRP KTGSQDQEVH 

       550        560        570        580        590        600 

RTVFMRLNII SPDLSGCTSK GVDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE 

       610        620        630        640        650        660 

VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE 

       670        680        690        700        710        720 

YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA 

       730        740        750        760        770        780 

VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ 

 

KSLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 86.4; Estimated by SDS-PAGE under reducing condition (kDa): 100-105 and 75-85 

 

Calculated PI: 6.24

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 80215

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity:  Binds to its ligands including TGFB1, TGFB3 and BMP9, and blocks the ligand-induced signaling activities.

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.