CNTFR or CNTFRa (Ciliary neurotrophic factor receptor subunit alpha) is a GPI-anchored surface glycoprotein of the type I cytokine receptor family, type 3 subfamily.  CNTFR consists of an extracellular region with 1 Ig-like C2-type (immunoglobulin-like) domain, 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, in addition to a transmembrane (TM) domain, and a cytoplasmic tail. The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding. CNTF is one of the cytokines including IL6, LIF, OSM, CNTF, IL11 and CT1 that utilize the common signaling transducer IL6ST/gp130 for initiating signal transmission. Binding of CNTF to CNTFR recruits the transmembrane components of the co-receptors, IL6ST/gp130 and leukemia inhibitory factor receptor (LIFR), facilitating signal transduction. The CNTF signaling pathway plays a critical role in neuronal cell survival, differentiation and gene expression. Single nucleotide polymorphisms in the CNTFR gene may be associated with variations in muscle strength, as well as early onset of eating disorders. 

CNTFR or CNTFRa (Ciliary neurotrophic factor receptor subunit alpha) is a GPI-anchored surface glycoprotein of the type I cytokine receptor family, type 3 subfamily.  CNTFR consists of an extracellular region with 1 Ig-like C2-type (immunoglobulin-like) domain, 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, in addition to a transmembrane (TM) domain, and a cytoplasmic tail. The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding. CNTF is one of the cytokines including IL6, LIF, OSM, CNTF, IL11 and CT1 that utilize the common signaling transducer IL6ST/gp130 for initiating signal transmission. Binding of CNTF to CNTFR recruits the transmembrane components of the co-receptors, IL6ST/gp130 and leukemia inhibitory factor receptor (LIFR), facilitating signal transduction. The CNTF signaling pathway plays a critical role in neuronal cell survival, differentiation and gene expression. Single nucleotide polymorphisms in the CNTFR gene may be associated with variations in muscle strength, as well as early onset of eating disorders. 

 

Product Details

 

Gene Symbol: CNTFR; CNTFRA; CNTFR-alpha

 

NCBI Gene ID: 1271

 

Uniprot Entry: P26992

 

Construct Details: The recombinant human CNTFR-Fc protein is expressed as a 545 amino acid protein consisting of Gln23 - Glu339 region of CNTFR and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

  

Source: Human cells stably expressing human CNTFR-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QRHSPQEAPH VQYERLGSDV TLPCGTANWD AAVTWRVNGT DLAPDLLNGS QLVLHGLELG 

        70         80         90        100        110        120 

HSGLYACFHR DSWHLRHQVL LHVGLPPREP VLSCRSNTYP KGFYCSWHLP TPTYIPNTFN 

       130        140        150        160        170        180 

VTVLHGSKIM VCEKDPALKN RCHIRYMHLF STIKYKVSIS VSNALGHNAT AITFDEFTIV 

       190        200        210        220        230        240 

KPDPPENVVA RPVPSNPRRL EVTWQTPSTW PDPESFPLKF FLRYRPLILD QWQHVELSDG 

       250        260        270        280        290        300 

TAHTITDAYA GKEYIIQVAA KDNEIGTWSD WSVAAHATPW TEEPRHLTTE AQAAETTTST 

       310        320        330        340        350        360 

TSSLAPPPTT KICDPGESTG THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 

       370        380        390        400        410        420 

VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 

       430        440        450        460        470        480 

VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 

       490        500        510        520        530        540 

SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS 

 

LSPGK 

  

M.W.: Calculated molecular mass (kDa): 61.1; Estimated by SDS-PAGE under reducing condition (kDa): 65-70 

 

Calculated PI: 6.48

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 106060

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.