CD362, also known as SDC2 (syndecan 2), Fibroglycan and HSPG (heparan sulfate proteoglycan core protein), is a transmembrane (type I) heparan sulfate proteoglycan of the syndecan proteoglycan family. The syndecans mediate cell adhesion, signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. CD362 is a 201 amino acid (aa) single pass type I transmembrane protein. CD362 contains an N-terminal signal peptide, a 126-aa extracellular domain (ECD) with 4 potential glycosylation sites, a transmembrane domain (TM), and a short 32-aa cytoplasmic tail. CD362 may act as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. CD362 may also regulates dendritic arbor morphogenesis.  Altered syndecan-2 expression has been detected in several different tumor types. 

CD362, also known as SDC2 (syndecan 2), Fibroglycan and HSPG (heparan sulfate proteoglycan core protein), is a transmembrane (type I) heparan sulfate proteoglycan of the syndecan proteoglycan family. The syndecans mediate cell adhesion, signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. CD362 is a 201 amino acid (aa) single pass type I transmembrane protein. CD362 contains an N-terminal signal peptide, a 126-aa extracellular domain (ECD) with 4 potential glycosylation sites, a transmembrane domain (TM), and a short 32-aa cytoplasmic tail. CD362 may act as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. CD362 may also regulates dendritic arbor morphogenesis.  Altered syndecan-2 expression has been detected in several different tumor types. 

 

Product Details

 

Gene Symbol: SDC2; SDC-2; CD362; SYND2; syndecan2; HSPG; HSPG1

 

NCBI Gene ID: 6383

 

Uniprot Entry: P34741

 

Construct Details: The recombinant human SDC2-Fc is expressed as a 349-amino acid protein consisting of  Glu19 - Leu139 region of SDC2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

 

Source: Human cells stably expressing human SDC2-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

ESRAELTSDK DMYLDNSSIE EASGVYPIDD DDYASASGSG ADEDVESPEL TTSRPLPKIL 

        70         80         90        100        110        120 

LTSAAPKVET TTLNIQNKIP AQTKSPEETD KEKVHLSDSE RKMDPAEEDT NVYTEKHSDS 

       130        140        150        160        170        180 

LSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY 

       190        200        210        220        230        240 

VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK 

       250        260        270        280        290        300 

AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL 

       310        320        330        340 

DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK 

  

M.W.: Calculated molecular mass (kDa): 38.8; Estimated by SDS-PAGE under reducing condition (kDa): 50-65

 

Calculated PI: 5.02

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  41745

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S" and "M" for marker)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.