Nectin4 is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Immunoglobulin superfamily (IgSF).  Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Nectin4 contains 1 Ig-like V-type domain and 2 Ig-like C2-type domains in the extracellular region that promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin4 and Nectin1. A soluble form of Nectin4 can be produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted Nectin4 is found in both breast tumor cell lines and breast tumor patients. Nectin4 acts as a receptor for measles virus but not for alpha-herpesvirus entry into cells.  Mutations in the Nectin4 gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. 

Nectin4 is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Immunoglobulin superfamily (IgSF).  Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Nectin4 contains 1 Ig-like V-type domain and 2 Ig-like C2-type domains in the extracellular region that promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin4 and Nectin1. A soluble form of Nectin4 can be produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted Nectin4 is found in both breast tumor cell lines and breast tumor patients. Nectin4 acts as a receptor for measles virus but not for alpha-herpesvirus entry into cells.  Mutations in the Nectin4 gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. 

 

Product Details

 

Gene Symbol: Nectin4; LNIR; PRR4; EDSS1; PVRL4; nectin-4

 

NCBI Gene ID: 81607

 

Uniprot Entry: Q96NY8

 

Construct Details: The recombinant human Nectin4 ECD protein is expressed as a 329-amino acid active form consisting of Gly32 - Ser349 region of Nectin4 and a C-terminal His-tag. Nectin4 ECD contains 1 potential N-linked glycosylation sites.

 

Source: Human cells stably expressing Nectin4 ECD and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

GELETSDVVT VVLGQDAKLP CFYRGDSGEQ VGQVAWARVD AGEGAQELAL LHSKYGLHVS 

        70         80         90        100        110        120 

PAYEGRVEQP PPPRNPLDGS VLLRNAVQAD EGEYECRVST FPAGSFQARL RLRVLVPPLP 

       130        140        150        160        170        180 

SLNPGPALEE GQGLTLAASC TAEGSPAPSV TWDTEVKGTT SSRSFKHSRS AAVTSEFHLV 

       190        200        210        220        230        240 

PSRSMNGQPL TCVVSHPGLL QDQRITHILH VSFLAEASVR GLEDQNLWHI GREGAMLKCL 

       250        260        270        280        290        300 

SEGQPPPSYN WTRLDGPLPS GVRVDGDTLG FPPLTTEHSG IYVCHVSNEF SSRDSQVTVD 

       310        320 

VLDPQEDSGK QVDLVSASST GHHHHHHHH 

 

  

M.W.: Calculated molecular mass (kDa): 35.5; Estimated by SDS-PAGE under reducing condition (kDa): 40-45

 

Calculated PI: 5.57

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  31315

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not available

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.