T-cell surface glycoprotein CD3 epsilon chain, also known as CD3e, T3e, TCRe and IMD18, is a single-pass type I transmembrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3e, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor (TCR) alpha/beta and gamma/delta heterodimers, forms the TCR-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways leading to the activation of T cells. CD3e plays an essential role in T-cell development. In CD3e-deficient mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated. Mutations in the CD3e gene are associated with immunodeficiency 18 (IMD18), an autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood. The CD3e gene has also been linked to a susceptibility to type I diabetes in women.

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3e, T3e, TCRe and IMD18, is a single-pass type I transmembrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3e, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor (TCR) alpha/beta and gamma/delta heterodimers, forms the TCR-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways leading to the activation of T cells. CD3e plays an essential role in T-cell development. In CD3e-deficient mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated. Mutations in the CD3e gene are associated with immunodeficiency 18 (IMD18), an autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood. The CD3e gene has also been linked to a susceptibility to type I diabetes in women.

 

Product Details

 

Gene Symbol: CD3E; T3E; TCRE; IMD18; Leu-4

 

NCBI Gene ID: 916

 

Uniprot Entry: P07766

 

Construct Details: Expressed as the combination of a heavy chain (HC) containing VH from anti-human CD3e mAb and CH region of mouse IgG1 and a mouse kappa light chain (LC) encoding VL from anti-CD3e mAb.  Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.

 

Source: Mammalian cells stably expressing anti-CD3e heavy chain and light chain (in mouse IgG1 format) and growing in chemical-defined media with no animal components. 

 

M.W.: Calculated molecular mass (kDa): 147 (49 kDa for a single HC, 24.5 kDa for a single LC); Estimated by SDS-PAGE under non-reducing condition(kDa): 150

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 1.0 mg/ml in sterile PBS pH7.4 without any carrier protein or preservative (the concentration determined by UV absorption with an A280nm of 1.0 for 0.73 mg/ml of IgG and verified by SDS-PAGE and Coomassie blue staining)

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Recognizes T cell surface marker CD3e by flow cytometry, IHC staining and fluorescent microscopy. Has the same binding specificity as anti-CD3e OKT3 mAbs and recombinant anti-CD3e mAbs, human IgG1 (SKU#MAB0818), and rabbit IgG (SKU#MAB1897).

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Nature 321:431 (1986).

2. Nat. Genet. 3:77 (1993).

3. J. Clin. Invest. 114:1512 (2004).

4. Proc. Natl. Acad. Sci. 101:7675 (2004).

5. J Immunol. 183: 1055 (2009).

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: MAB1899-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.