TMPRSS1 (Transmembrane serine protease 1) or HPN (Hepsin) is a serine protease that belongs to the peptidase S1 family. TMPRSS1 is a type II transmembrane protein with an extracellular region containing a scavenger receptor cysteine-rich (SRCR) domain and a protease domain. TMPRSS1 cleaves after basic amino-acid residues, with Arg strongly preferred to Lys.  TMPRSS1 can be autocleaved into a non-catalytic SRCR chain and a catalytic serine protease chain, which associate via a single disulfide bond. TMPRSS1 contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL, as well as ACE2  (Angiotensin-converting enzyme 2) and urinary Uromodulin (UMOD) required for UMOD polymerization. TMPRSS1 is involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Its expression is associated with the growth and progression of cancers, particularly prostate cancer.

TMPRSS1 (Transmembrane serine protease 1) or HPN (Hepsin) is a serine protease that belongs to the peptidase S1 family. TMPRSS1 is a type II transmembrane protein with an extracellular region containing a scavenger receptor cysteine-rich (SRCR) domain and a protease domain. TMPRSS1 cleaves after basic amino-acid residues, with Arg strongly preferred to Lys.  TMPRSS1 can be autocleaved into a non-catalytic SRCR chain and a catalytic serine protease chain, which associate via a single disulfide bond. TMPRSS1 contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL, as well as ACE2  (Angiotensin-converting enzyme 2) and urinary Uromodulin (UMOD) required for UMOD polymerization. TMPRSS1 is involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Its expression is associated with the growth and progression of cancers, particularly prostate cancer.

 

Product Details

 

Gene Symbol: TMPRSS1; HPN; Hepsin

 

NCBI Gene ID: 3249

 

Uniprot Entry: P05981

 

Construct Details: Full length human TMPRSS1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1254 (bp)

 

Gene Insert Sequence:  

ATGGCGCAGAAGGAGGGTGGCCGGACTGTGCCATGCTGCTCCAGACCCAAGGTGGCAGCTCTCACTGCGG

GGACCCTGCTACTTCTGACAGCCATCGGGGCGGCATCCTGGGCCATTGTGGCTGTTCTCCTCAGGAGTGA

CCAGGAGCCGCTGTACCCAGTGCAGGTCAGCTCTGCGGACGCTCGGCTCATGGTCTTTGACAAGACGGAA

GGGACGTGGCGGCTGCTGTGCTCCTCGCGCTCCAACGCCAGGGTAGCCGGACTCAGCTGCGAGGAGATGG

GCTTCCTCAGGGCACTGACCCACTCCGAGCTGGACGTGCGAACGGCGGGCGCCAATGGCACGTCGGGCTT

CTTCTGTGTGGACGAGGGGAGGCTGCCCCACACCCAGAGGCTGCTGGAGGTCATCTCCGTGTGTGATTGC

CCCAGAGGCCGTTTCTTGGCCGCCATCTGCCAAGACTGTGGCCGCAGGAAGCTGCCCGTGGACCGCATCG

TGGGAGGCCGGGACACCAGCTTGGGCCGGTGGCCGTGGCAAGTCAGCCTTCGCTATGATGGAGCACACCT

CTGTGGGGGATCCCTGCTCTCCGGGGACTGGGTGCTGACAGCCGCCCACTGCTTCCCGGAGCGGAACCGG

GTCCTGTCCCGATGGCGAGTGTTTGCCGGTGCCGTGGCCCAGGCCTCTCCCCACGGTCTGCAGCTGGGGG

TGCAGGCTGTGGTCTACCACGGGGGCTATCTTCCCTTTCGGGACCCCAACAGCGAGGAGAACAGCAACGA

TATTGCCCTGGTCCACCTCTCCAGTCCCCTGCCCCTCACAGAATACATCCAGCCTGTGTGCCTCCCAGCT

GCCGGCCAGGCCCTGGTGGATGGCAAGATCTGTACCGTGACGGGCTGGGGCAACACGCAGTACTATGGCC

AACAGGCCGGGGTACTCCAGGAGGCTCGAGTCCCCATAATCAGCAATGATGTCTGCAATGGCGCTGACTT

CTATGGAAACCAGATCAAGCCCAAGATGTTCTGTGCTGGCTACCCCGAGGGTGGCATTGATGCCTGCCAG

GGCGACAGCGGTGGTCCCTTTGTGTGTGAGGACAGCATCTCTCGGACGCCACGTTGGCGGCTGTGTGGCA

TTGTGAGTTGGGGCACTGGCTGTGCCCTGGCCCAGAAGCCAGGCGTCTACACCAAAGTCAGTGACTTCCG

GGAGTGGATCTTCCAGGCCATAAAGACTCACTCCGAAGCCAGCGGCATGGTGACCCAGCTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

  

 

Product datasheet (pdf) and additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.