LTB (lymphotoxin beta) or TNFC (tumor necrosis factor c) is a member of the tumor necrosis factor superfamily (TNFSF3). LTB/TNFSF2 is a single-pass type II transmebrane glycoprotein consisting of a short cytoplasmic domain (1-18 aa), a transmembrane domain (TM: 19-48 aa) and a 196 aa extracellular domain (ECD: 49-244 aa). LTB/TNFSF3 anchors LTA/TNFSF1 (lymphotoxin-alpha) to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the LTA1/LTB2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the LTBR (lymphotoxin-beta receptor). The minor complex is LTA2/LTB1. LTB/TNFSF3 is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. 

LTB (lymphotoxin beta) or TNFC (tumor necrosis factor c) is a member of the tumor necrosis factor superfamily (TNFSF3). LTB/TNFSF2 is a single-pass type II transmebrane glycoprotein consisting of a short cytoplasmic domain (1-18 aa), a transmembrane domain (TM: 19-48 aa) and a 196 aa extracellular domain (ECD: 49-244 aa). LTB/TNFSF3 anchors LTA/TNFSF1 (lymphotoxin-alpha) to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the LTA1/LTB2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the LTBR (lymphotoxin-beta receptor). The minor complex is LTA2/LTB1. LTB/TNFSF3 is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. 

  

Product Details

 

Gene Symbol: TNFSF3;  LTB; TNFC; p33; TNF-C; TNFSF-3; TNLG1C

  

NCBI Gene ID: 4050

 

Uniprot Entry: Q06643

 

Construct Details: The recombinant human LTB-Fc is expressed as a 425 amino acid protein consisting of Gln49 - Gly244 region of LTB (UniProt accession #Q06643) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing LTB-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QDQGGLVTET ADPGAQAQQG LGFQKLPEEE PETDLSPGLP AAHLIGAPLK GQGLGWETTK  

        70         80         90        100        110        120 

EQAFLTSGTQ FSDAEGLALP QDGLYYLYCL VGYRGRAPPG GGDPQGRSVT LRSSLYRAGG  

       130        140        150        160        170        180 

AYGPGTPELL LEGAETVTPV LDPARRQGYG PLWYTSVGFG GLVQLRRGER VYVNISHPDM  

       190        200        210        220        230        240 

VDFARGKTFF GAVMVGSGTG THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV  

       250        260        270        280        290        300 

VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK  

       310        320        330        340        350        360 

VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 

       370        380        390        400        410        420 

SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS 

 

LSPGK 

 

 

  

M.W.: Calculated molecular mass (kDa): 46.4; Estimated by SDS-PAGE under reducing condition (kDa): ~55-60

 

Calculated PI: 5.91

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  60195

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.