IL31RA (interleukin-31 receptor subunit alpha) is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  IL31R consists of an extracellular region with 5 fibronectin type III (FN3) domains, a transmembrane domain, and a cytoplasmic tail. IL31RA functions either as a monomer, or as part of a receptor complex with OSMR (oncostatin M receptor). OSMR heterodimerizes with IL6ST (interleukin 6 signal transducer) or gp130 to form the type II OSMR complex and with IL31RA to form the IL31R complex, and thus transduces oncostatin M and IL31 induced signaling events.  IL31RA, with homology to IL6ST/gp130, is expressed on monocytes, and is involved in IL31 signaling via activation of STAT-3 and STAT-5.  Mutations in the IL31RA gene have been associated with familial primary localized cutaneous amyloidosis, 2 (PLCA2). 

IL31RA (interleukin-31 receptor subunit alpha) is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  IL31R consists of an extracellular region with 5 fibronectin type III (FN3) domains, a transmembrane domain, and a cytoplasmic tail. IL31RA functions either as a monomer, or as part of a receptor complex with OSMR (oncostatin M receptor). OSMR heterodimerizes with IL6ST (interleukin 6 signal transducer) or gp130 to form the type II OSMR complex and with IL31RA to form the IL31R complex, and thus transduces oncostatin M and IL31 induced signaling events.  IL31RA, with homology to IL6ST/gp130, is expressed on monocytes, and is involved in IL31 signaling via activation of STAT-3 and STAT-5.  Mutations in the IL31RA gene have been associated with familial primary localized cutaneous amyloidosis, 2 (PLCA2). 

 

Product Details

 

Gene Symbol: IL31RA; CRL; GPL; CRL3; GLMR; GLM-R; PLCA2; hGLM-R; IL-31RA; IL-31Ralpha; ZcytoR17; PRO21384

 

NCBI Gene ID: 133396

 

Uniprot Entry: Q8NI17

 

Construct Details: The recombinant human IL31RA-Fc fusion is expressed as a 728 amino acid protein consisting of Ala20 - Glu519 region of IL31RA and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.

   

Source: Human cells stably expressing IL31RA-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
ALPAKPENIS CVYYYRKNLT CTWSPGKETS YTQYTVKRTY AFGEKHDNCT TNSSTSENRA

        70         80         90        100        110        120
SCSFFLPRIT IPDNYTIEVE AENGDGVIKS HMTYWRLENI AKTEPPKIFR VKPVLGIKRM

       130        140        150        160        170        180
IQIEWIKPEL APVSSDLKYT LRFRTVNSTS WMEVNFAKNR KDKNQTYNLT GLQPFTEYVI

       190        200        210        220        230        240
ALRCAVKESK FWSDWSQEKM GMTEEEAPCG LELWRVLKPA EADGRRPVRL LWKKARGAPV

       250        260        270        280        290        300
LEKTLGYNIW YYPESNTNLT ETMNTTNQQL ELHLGGESFW VSMISYNSLG KSPVATLRIP

       310        320        330        340        350        360
AIQEKSFQCI EVMQACVAED QLVVKWQSSA LDVNTWMIEW FPDVDSEPTT LSWESVSQAT

       370        380        390        400        410        420
NWTIQQDKLK PFWCYNISVY PMLHDKVGEP YSIQAYAKEG VPSEGPETKV ENIGVKTVTI

       430        440        450        460        470        480
TWKEIPKSER KGIICNYTIF YQAEGGKGFS KTVNSSILQY GLESLKRKTS YIVQVMASTS

       490        500        510        520        530        540
AGGTNGTSIN FKTLSFSVFE STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV

       550        560        570        580        590        600
TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY

       610        620        630        640        650        660
KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV

       670        680        690        700        710        720
EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK


SLSLSPGK

 

 

M.W.: Calculated molecular mass 82.7 kDa; estimated by SDS-PAGE under reducing condition: ~100-110 kDa 

 

Calculated PI: 8.16

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  164180

  

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.