IL15RA (interleukin 15 receptor subunit alpha), also known as CD215, is a single-pass type I transmembrane glycoprotein. IL15RA lacks structural features characteristic of members of the cytokine receptor superfamily. IL15RA contains only 1 Sushi (CCP/SCR) domains in the extracellular region. IL15RA is a cytokine receptor that specifically binds IL15 with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R-beta and IL2R-gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. IL15RA is structurally related to IL2RA/IL2Ralpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. IL15RA can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms of IL15RA may alter or interfere with signal transduction. Among these isoforms, Isoform 5, 6, 7 and 8 do not bind IL15. A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor and also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.

IL15RA (interleukin 15 receptor subunit alpha), also known as CD215, is a single-pass type I transmembrane glycoprotein. IL15RA lacks structural features characteristic of members of the cytokine receptor superfamily. IL15RA contains only 1 Sushi (CCP/SCR) domains in the extracellular region. IL15RA is a cytokine receptor that specifically binds IL15 with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R-beta and IL2R-gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. IL15RA is structurally related to IL2RA/IL2Ralpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. IL15RA can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms of IL15RA may alter or interfere with signal transduction. Among these isoforms, Isoform 5, 6, 7 and 8 do not bind IL15. A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor and also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.

 

Product Details

 

Gene Symbol: IL15RA; CD215

 

NCBI Gene ID: 3601

 

Uniprot Entry: P13261

 

Construct Details: The recombinant human IL15RA-Fc fusion is expressed as a 403 amino acid protein consisting of Ilu31 - Thr205 region of IL15RA and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

   

Source: Human cells stably expressing IL15RA-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
ITCPPPMSVE HADIWVKSYS LYSRERYICN SGFKRKAGTS SLTECVLNKA TNVAHWTTPS

        70         80         90        100        110        120
LKCIRDPALV HQRPAPPSTV TTAGVTPQPE SLSPSGKEPA ASSPSSNNTA ATTAAIVPGS

       130        140        150        160        170        180
QLMPSKSPST GTTEISSHES SHGTPSQTTA KNWELTASAS HQPPGVYPQG HSDTTSTGTH

       190        200        210        220        230        240
TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV

       250        260        270        280        290        300
HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR

       310        320        330        340        350        360
EPQVYTLPPS REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF

       370        380        390        400
FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK  

 

M.W.: Calculated molecular mass 44 kDa; estimated by SDS-PAGE under reducing condition: ~65-70 kDa 

 

Calculated PI: 8.02

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  58495

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.