ENPP4 (ectonucleotide pyrophosphatase/phosphodiesterase family member 4) is a member of the nucleotide pyrophosphatase/ phosphodiesterase family that are involved in hydrolysis of extracellular nucleotides. Typically, this family ectoenzymes are single-pass type II transmembrane proteins with ATPase and ATP pyrophosphatase activities. Unlike other members, ENPP4 is a single pass type I transmembrane glycoprotein. ENPP4 enables bis(5'-adenosyl)-triphosphatase activity, and hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. Ap3A and Ap4A are diadenosine polyphosphates thought to induce proliferation of vascular smooth muscle cells. ENPP4 is involved in positive regulation of blood coagulation and purine ribonucleoside catabolic process. It acts as a procoagulant, mediating platelet aggregation at the site of nascent thrombus via release of ADP from Ap3A and activation of ADP receptors.

ENPP4 (ectonucleotide pyrophosphatase/phosphodiesterase family member 4) is a member of the nucleotide pyrophosphatase/ phosphodiesterase family that are involved in hydrolysis of extracellular nucleotides. Typically, this family ectoenzymes are single-pass type II transmembrane proteins with ATPase and ATP pyrophosphatase activities. Unlike other members, ENPP4 is a single pass type I transmembrane glycoprotein. ENPP4 enables bis(5'-adenosyl)-triphosphatase activity, and hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. Ap3A and Ap4A are diadenosine polyphosphates thought to induce proliferation of vascular smooth muscle cells. ENPP4 is involved in positive regulation of blood coagulation and purine ribonucleoside catabolic process. It acts as a procoagulant, mediating platelet aggregation at the site of nascent thrombus via release of ADP from Ap3A and activation of ADP receptors.

 

Product Details

 

Gene Symbol: ENPP4; NPP4; AP3Aase; KIAA0879

 

NCBI Gene ID: 22875

 

Uniprot Entry: Q9Y6X5

 

Construct Details: Full length human ENPP4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1362 (bp)

 

Gene Insert Sequence:  

ATGAAGTTATTAGTAATACTTTTGTTTTCTGGACTTATAACTGGTTTTAGAAGTGACTCTTCCTCTAGTTTGCCACCTAA
GTTACTACTAGTATCCTTTGATGGCTTCAGAGCTGATTATCTGAAGAACTATGAATTTCCTCATCTCCAGAATTTTATCA
AAGAAGGTGTTTTGGTAGAGCATGTTAAAAATGTTTTTATCACAAAAACATTTCCAAACCACTACAGTATTGTGACAGGC
TTGTATGAAGAAAGCCATGGCATTGTGGCTAATTCCATGTATGATGCAGTCACAAAGAAACACTTTTCTGACTCTAATGA
CAAGGATCCTTTTTGGTGGAATGAGGCAGTACCTATTTGGGTGACCAATCAGCTTCAGGAAAACAGATCAAGTGCTGCTG
CTATGTGGCCTGGTACTGATGTACCCATTCACGATACCATCTCTTCCTATTTTATGAATTACAACTCCTCAGTGTCATTT
GAGGAAAGACTAAATAATATTACTATGTGGCTAAACAATTCGAACCCACCAGTCACCTTTGCAACACTATATTGGGAAGA
ACCAGATGCAAGTGGCCACAAATACGGACCTGAAGATAAAGAAAACATGAGCAGAGTGTTGAAAAAAATAGATGATCTTA
TCGGTGACTTAGTCCAAAGACTCAAGATGTTAGGGCTATGGGAAAATCTTAATGTGATCATTACAAGTGATCATGGGATG
ACCCAGTGTTCTCAGGACAGACTGATAAACCTGGATTCCTGCATCGATCATTCATACTACACTCTTATAGATTTGAGCCC
AGTTGCTGCAATACTTCCCAAAATAAATAGAACAGAGGTTTATAACAAACTGAAAAACTGTAGCCCTCATATGAATGTTT
ATCTCAAAGAAGACATTCCTAACAGATTTTATTACCAACATAATGATCGAATTCAGCCCATTATTTTGGTTGCCGATGAA
GGCTGGACAATTGTGCTAAATGAATCATCACAAAAATTAGGTGACCATGGTTATGATAATTCTTTGCCTAGTATGCATCC
ATTTCTAGCTGCCCACGGACCTGCATTTCACAAAGGCTACAAGCATAGCACAATTAACATTGTGGATATTTATCCAATGA
TGTGCCACATCCTGGGATTAAAACCACATCCCAATAATGGGACCTTTGGTCATACTAAGTGCTTGTTAGTTGACCAGTGG
TGCATTAATCTCCCAGAAGCCATCGCGATTGTTATCGGTTCACTCTTGGTGTTAACCATGCTAACATGCCTCATAATAAT
CATGCAGAATAGACTTTCTGTACCTCGTCCATTTTCTCGACTTCAGCTACAAGAAGATGATGATGATCCTTTAATTGGGT
GA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Documentation

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.