ENPP6 (ectonucleotide pyrophosphatase/phosphodiesterase family member 6) is a member of the nucleotide pyrophosphatase/ phosphodiesterase family that are involved in hydrolysis of extracellular nucleotides. Typically, this family ectoenzymes are single-pass type II transmembrane proteins with ATPase and ATP pyrophosphatase activities. Unlike other members, ENPP6 is a GPI (glycosyl-phosphatidylinositol)-anchored glycoprotein, located in extracellular region and plasma membrane. ENPP6 is a choline-specific glycerophosphodiesterase that hydrolyzes glycerophosphocholine (GPC) and lysophosphatidylcholine (LPC).  ENPP6 is involved in choline metabolic process and lipid metabolic process, contributing to supplying choline to the cells.

ENPP6 (ectonucleotide pyrophosphatase/phosphodiesterase family member 6) is a member of the nucleotide pyrophosphatase/ phosphodiesterase family that are involved in hydrolysis of extracellular nucleotides. Typically, this family ectoenzymes are single-pass type II transmembrane proteins with ATPase and ATP pyrophosphatase activities. Unlike other members, ENPP6 is a GPI (glycosyl-phosphatidylinositol)-anchored glycoprotein, located in extracellular region and plasma membrane. ENPP6 is a choline-specific glycerophosphodiesterase that hydrolyzes glycerophosphocholine (GPC) and lysophosphatidylcholine (LPC).  ENPP6 is involved in choline metabolic process and lipid metabolic process, contributing to supplying choline to the cells.

 

Product Details

 

Gene Symbol: ENPP6; NPP6; GPC-Cpde; UNQ1889/PRO4334

 

NCBI Gene ID: 133121

 

Uniprot Entry: Q6UWR7

 

Construct Details: Full length human ENPP6 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1323 (bp)

 

Gene Insert Sequence:  

ATGGCAGTGAAGCTTGGGACCCTCCTGCTGGCCCTTGCCCTGGGCCTGGCCCAGCCAGCCTCTGCCCGCCGGAAGCTGCT
GGTGTTTCTGCTGGATGGTTTTCGCTCAGACTACATCAGTGATGAGGCGCTGGAGTCATTGCCTGGTTTCAAAGAGATTG
TGAGCAGGGGAGTAAAAGTGGATTACTTGACTCCAGACTTCCCTAGTCTCTCGTATCCCAATTATTATACCCTAATGACT
GGCCGCCATTGTGAAGTCCATCAGATGATCGGGAACTACATGTGGGACCCCACCACCAACAAGTCCTTTGACATTGGCGT
CAACAAAGACAGCCTAATGCCTCTCTGGTGGAATGGATCAGAACCTCTGTGGGTCACTCTGACCAAGGCCAAAAGGAAGG
TCTACATGTACTACTGGCCAGGCTGTGAGGTTGAGATTCTGGGTGTCAGACCCACCTACTGCCTAGAATATAAAAATGTC
CCAACGGATATCAATTTTGCCAATGCAGTCAGCGATGCTCTTGACTCCTTCAAGAGTGGCCGGGCCGACCTGGCAGCCAT
ATACCATGAGCGCATTGACGTGGAAGGCCACCACTACGGGCCTGCATCTCCGCAGAGGAAAGATGCCCTCAAGGCTGTAG
ACACTGTCCTGAAGTACATGACCAAGTGGATCCAGGAGCGGGGCCTGCAGGACCGCCTGAACGTCATTATTTTCTCGGAT
CACGGAATGACCGACATTTTCTGGATGGACAAAGTGATTGAGCTGAATAAGTACATCAGCCTGAATGACCTGCAGCAAGT
GAAGGACCGCGGGCCTGTTGTGAGCCTTTGGCCGGCCCCTGGGAAACACTCTGAGATATATAACAAACTGAGCACAGTGG
AACACATGACTGTCTACGAGAAAGAAGCCATCCCAAGCAGGTTCTATTACAAGAAAGGAAAGTTTGTCTCTCCTTTGACT
TTAGTGGCTGATGAAGGCTGGTTCATAACTGAGAATCGAGAGATGCTTCCGTTTTGGATGAACAGCACCGGCAGGCGGGA
AGGTTGGCAGCGTGGATGGCACGGCTACGACAACGAGCTCATGGACATGCGGGGCATCTTCCTGGCCTTCGGACCTGATT
TCAAATCCAACTTCAGAGCTGCTCCTATCAGGTCGGTGGACGTCTACAATGTCATGTGCAATGTGGTGGGCATCACCCCG
CTGCCCAACAACGGATCCTGGTCCAGGGTGATGTGCATGCTGAAGGGCCGCGCCAGCACTGCCCCGCCTGTCTGGCCCAG
CCACTGTGCCCTGGCACTGATTCTTCTCTTCCTGCTTGCATAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Documentation

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.