ASTN2 (Astrotactin-2) is a two-pass type I transmembrane protein that belongs to the astrotactin family.  ASTN2 contains 2 extracellular domains (ECD), including a 157 aa (amino acid) long small ECD1 and a 884 aa long large ECD2.  The ECD2 is composed of three EGF-like motifs and one Fibronectin type-III motif.  The C-terminal region after the fibronectin type-III domain presents structural similarity to annexin domains and binds calcium ions. ASTN2 is expressed in the brain and may function in neuronal migration, based on functional studies of the related ASTN1.  ASTN2 mediates recycling of the neuronal cell adhesion molecule ASTN1 to the anterior pole of the cell membrane in migrating neurons. ASTN2 also promotes ASTN1 internalization and intracellular transport of endocytosed ASTN1. ASTN2 binds inositol-4,5-bisphosphate, inositol-3,4,5-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, suggesting it is recruited to membranes lipids with a phosphoinositide headgroup. A deletion at the ASTN2 locus has been associated with schizophrenia.

ASTN2 (Astrotactin-2) is a two-pass type I transmembrane protein that belongs to the astrotactin family.  ASTN2 contains 2 extracellular domains (ECD), including a 157 aa (amino acid) long small ECD1 and a 884 aa long large ECD2.  The ECD2 is composed of three EGF-like motifs and one Fibronectin type-III motif.  The C-terminal region after the fibronectin type-III domain presents structural similarity to annexin domains and binds calcium ions. ASTN2 is expressed in the brain and may function in neuronal migration, based on functional studies of the related ASTN1.  ASTN2 mediates recycling of the neuronal cell adhesion molecule ASTN1 to the anterior pole of the cell membrane in migrating neurons. ASTN2 also promotes ASTN1 internalization and intracellular transport of endocytosed ASTN1. ASTN2 binds inositol-4,5-bisphosphate, inositol-3,4,5-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, suggesting it is recruited to membranes lipids with a phosphoinositide headgroup. A deletion at the ASTN2 locus has been associated with schizophrenia.

 

Product Details

 

Gene Symbol: ASTN2; Astrotactin-2

 

NCBI Gene ID: 23245

 

Uniprot Entry: O75129

 

Construct Details: The recombinant human ASTN2 ECD2 protein is expressed as a 709-amino acid protein consisting of the region Val601- Arg1288 of ASTN2 (Uniprot# O75129-2) and a C-terminal His-tag.

   

Source: Human cells stably expressing ASTN2 ECD2 and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
VRDCSRNNGG CTRNFKCVSD RQVDSSGCVC PEELKPMKDG SGCYDHSKGI DCSDGFNGGC

        70         80         90        100        110        120
EQLCLQQTLP LPYDATSSTI FMFCGCVEEY KLAPDGKSCL MLSDVCEGPK CLKPDSKFND

       130        140        150        160        170        180
TLFGEMLHGY NNRTQHVNQG QVFQMTFREN NFIKDFPQLA DGLLVIPLPV EEQCRGVLSE

       190        200        210        220        230        240
PLPDLQLLTG DIRYDEAMGY PMVQQWRVRS NLYRVKLSTI TLAAGFTNVL KILTKESSRE

       250        260        270        280        290        300
ELLSFIQHYG SHYIAEALYG SELTCIIHFP SKKVQQQLWL QYQKETTELG SKKELKSMPF

       310        320        330        340        350        360
ITYLSGLLTA QMLSDDQLIS GVEIRCEEKG RCPSTCHLCR RPGKEQLSPT PVLLEINRVV

       370        380        390        400        410        420
PLYTLIQDNG TKEAFKSALM SSYWCSGKGD VIDDWCRCDL SAFDANGLPN CSPLLQPVLR

       430        440        450        460        470        480
LSPTVEPSST VVSLEWVDVQ PAIGTKVSDY ILQHKKVDEY TDTDLYTGEF LSFADDLLSG

       490        500        510        520        530        540
LGTSCVAAGR SHGEVPEVSI YSVIFKCLEP DGLYKFTLYA VDTRGRHSEL STVTLRTACP

       550        560        570        580        590        600
LVDDNKAEEI ADKIYNLYNG YTSGKEQQMA YNTLMEVSAS MLFRVQHHYN SHYEKFGDFV

       610        620        630        640        650        660
WRSEDELGPR KAHLILRRLE RVSSHCSSLL RSAYIQSRVE TVPYLFCRSE EVRPAGMVWY

       670        680        690        700
SILKDTKITC EEKMVSMARN TYGESKGRST TENLYFQGST GHHHHHHHH

 

 

 

M.W.: Calculated molecular mass 80.0 kDa; estimated by SDS-PAGE under reducing condition: ~80-85 kDa 

 

Calculated PI: 5.83

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  86565

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.