TAJ (Toxicity and JNK inducer), also known as TRADE and TROY, is a single-pass type I membrane glycoprotein in the tumor necrosis factor receptor superfamily (TNFRSF19). TAJ contains 3 TNFR cysteine-rich repeats in the extracellular region but lacks a cytoplasmic death domain (DD). TAJ is widely expressed in the embryo, but shows restricted expression in adult. TAJ is detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles. TAJ has been shown to interact with TRAF family members to activate JNK signaling pathway when overexpressed in cells. TAJ is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. TAJ may be a component of the Nogo­A receptor that mediates axon repulsion initiated by one of five myelin­associated inhibitory factors. In the hair follicle, TROY binds Lymphotoxin-α and acts with EDA to drive secondary hair follicle development. TAJ has also been found on melanoma cells, not normal melanocytes.  TAJ has thus been identified as a biomarker for melanoma and represents a promising cell surface target for cell and antibody-based immunotherapies.

TAJ (Toxicity and JNK inducer), also known as TRADE and TROY, is a single-pass type I membrane glycoprotein in the tumor necrosis factor receptor superfamily (TNFRSF19). TAJ contains 3 TNFR cysteine-rich repeats in the extracellular region but lacks a cytoplasmic death domain (DD). TAJ is widely expressed in the embryo, but shows restricted expression in adult. TAJ is detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles. TAJ has been shown to interact with TRAF family members to activate JNK signaling pathway when overexpressed in cells. TAJ is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. TAJ may be a component of the Nogo­A receptor that mediates axon repulsion initiated by one of five myelin­associated inhibitory factors. In the hair follicle, TROY binds Lymphotoxin-α and acts with EDA to drive secondary hair follicle development. TAJ has also been found on melanoma cells, not normal melanocytes.  TAJ has thus been identified as a biomarker for melanoma and represents a promising cell surface target for cell and antibody-based immunotherapies.

  

Product Details

 

Gene Symbol: TNFRSF19; TROY; TRADE; TAJ-alpha; UNQ1888/PRO4333

  

NCBI Gene ID: 55504

 

Uniprot Entry: Q9NS68

 

Construct Details: The recombinant human TAJ-Fc fusion is expressed as a 368 amino acid protein consisting of Glu30 - Ala169 region of TAJ (UniProt accession #Q9NS68) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

  

Source: Human cells stably expressing human TAJ-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

 

        10         20         30         40         50         60 

ESGDCRQQEF RDRSGNCVPC NQCGPGMELS KECGFGYGED AQCVTCRLHR FKEDWGFQKC 

        70         80         90        100        110        120 

KPCLDCAVVN RFQKANCSAT SDAICGDCLP GFYRKTKLVG FQDMECVPCG DPPPPYEPHC 

       130        140        150        160        170        180 

ASKVNLVKIA STASSPRDTA STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV 

       190        200        210        220        230        240 

TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY 

        250        260        270        280        290        300 

KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV 

       310        320        330        340        350        360 

EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK 

 

SLSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 40.9; Estimated by SDS-PAGE under reducing condition (kDa): 50-55

 

Calculated PI: 6.82

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  46755

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not Available

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Genomics 62:103 (1999).

2. J. Biol. Chem. 275:15336 (2000).

3. J. Biol. Chem. 275:20742 (2000).

4. Neuron 45:345 (2005).

5. Hum. Mol. Genet. 17:3380 (2008).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0291-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.