ROR1 (receptor tyrosine kinase-like orphan receptor 1), also known as NTRKR1 (neurotrophic tyrosine kinase, receptor-related 1), is a single-pass type I transmembrane glycoprotein that belongs to the ROR subfamily of the tyrosine protein kinase family. The ROR subfamily members, ROR1 and ROR2, are tyrosine kinase receptors important in regulating skeletal and neuronal development, cell migration and cell polarity. Each ROR member contains 1 FZ (frizzled) domain, 1 Ig (immunoglobulin)-like C2-type domain, and 1 kringle domain in the extracellular region and 1 protein kinase domain in the cytoplasmic region. ROR1 is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with various hematological malignancies of both lymphoid and myeloid origins as well as in melanoma. ROR1 is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. ROR1 modulates neurite growth in the central nervous system. ROR1 may play a role in the survival of melanoma cells and regulate EMT and tumor metastasis.

ROR1 (receptor tyrosine kinase-like orphan receptor 1), also known as NTRKR1 (neurotrophic tyrosine kinase, receptor-related 1), is a single-pass type I transmembrane glycoprotein that belongs to the ROR subfamily of the tyrosine protein kinase family. The ROR subfamily members, ROR1 and ROR2, are tyrosine kinase receptors important in regulating skeletal and neuronal development, cell migration and cell polarity. Each ROR member contains 1 FZ (frizzled) domain, 1 Ig (immunoglobulin)-like C2-type domain, and 1 kringle domain in the extracellular region and 1 protein kinase domain in the cytoplasmic region. ROR1 is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with various hematological malignancies of both lymphoid and myeloid origins as well as in melanoma. ROR1 is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. ROR1 modulates neurite growth in the central nervous system. ROR1 may play a role in the survival of melanoma cells and regulate EMT and tumor metastasis.

 

Product Details

 

Gene Symbol: NTRKR1; dJ537F10.1

 

NCBI Gene ID: 4919

 

Uniprot Entry: Q01973

 

Construct Details: The recombinant human ROR1-Fc fusion is expressed as a 602 amino acid protein consisting of Gln30 - Glu403 region of ROR1 (Uniprot #Q01973) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).

 

Source:  Human cells stably expressing ROR1-Fc and growing in chemical-defined media with no animal components or antibiotics.

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

QETELSVSAE LVPTSSWNIS SELNKDSYLT LDEPMNNITT SLGQTAELHC KVSGNPPPTI  

        70         80         90        100        110        120 

RWFKNDAPVV QEPRRLSFRS TIYGSRLRIR NLDTTDTGYF QCVATNGKEV VSSTGVLFVK  

       130        140        150        160        170        180 

FGPPPTASPG YSDEYEEDGF CQPYRGIACA RFIGNRTVYM ESLHMQGEIE NQITAAFTMI 

       190        200        210        220        230        240 

GTSSHLSDKC SQFAIPSLCH YAFPYCDETS SVPKPRDLCR DECEILENVL CQTEYIFARS 

       250        260        270        280        290        300 

NPMILMRLKL PNCEDLPQPE SPEAANCIRI GIPMADPINK NHKCYNSTGV DYRGTVSVTK 

       310        320        330        340        350        360 

SGRQCQPWNS QYPHTHTFTA LRFPELNGGH SYCRNPGNQK EAPWCFTLDE NFKSDLCDIP 

       370        380        390        400        410        420 

ACDSKDSKEK NKMESTGTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD 

       430        440        450        460        470        480 

VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN 

       490        500        510        520        530        540 

KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG 

       550        560        570        580        590        600 

QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP 

 

GK

 

M.W.: Calculated molecular mass (kDa): 67.5; Estimated by SDS-PAGE under reducing condition (kDa): 75-85

 

Calculated PI: 5.90

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 79770

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not Available

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. J. Biol. Chem. 267:26181 (1992)

2. Oncogene 13:1555 (1996) 

3. Genomics. 41: 283 (1997).

4. Mol Cell Biol. 21: 8329 (2001).

5. Clin Cancer Res. 14: 396 (2008).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0043-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.