LTβR (Lymphotoxin beta receptor), also known as TNFR3 or TNF Rrp, is a single-pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF3). LTβR contains4 cysteine-rich repeats within the extracellular domain (ECD) characteristic of the TNFRSF. LTβR ligands include homotrimeric of LIGHT/TNFSF14 and heterotrimeric lymphotoxin-α1/β2. LTβR is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. LTβR is expressed on mesenchymal stromal organizing cells that give rise to stroma of primary (thymus), secondary (tonsils, lymph nodes and Peyers patches) and tertiary (ectopic inflammatory) lymphoid structures. Secondary immune tissues are absent in LTβR-deficient mice. Depending on the cell type, activation of LTβR has been shown to induce canonical or alternative NF-κB pathways. Soluble LTβR can be formed by proteolytic cleavage of the ECD, and act as an antagonist to inhibit autoimmunity. LTβR regulates cell growth and can initiate inflammation-related carcinogenesis. It is expressed in hepatocytes, is up-regulated in regeneration, hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis.

LTβR (Lymphotoxin beta receptor), also known as TNFR3 or TNF Rrp, is a single-pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF3). LTβR contains4 cysteine-rich repeats within the extracellular domain (ECD) characteristic of the TNFRSF. LTβR ligands include homotrimeric of LIGHT/TNFSF14 and heterotrimeric lymphotoxin-α1/β2. LTβR is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. LTβR is expressed on mesenchymal stromal organizing cells that give rise to stroma of primary (thymus), secondary (tonsils, lymph nodes and Peyers patches) and tertiary (ectopic inflammatory) lymphoid structures. Secondary immune tissues are absent in LTβR-deficient mice. Depending on the cell type, activation of LTβR has been shown to induce canonical or alternative NF-κB pathways. Soluble LTβR can be formed by proteolytic cleavage of the ECD, and act as an antagonist to inhibit autoimmunity. LTβR regulates cell growth and can initiate inflammation-related carcinogenesis. It is expressed in hepatocytes, is up-regulated in regeneration, hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis.

  

Product Details

 

Gene Symbol: TNFRSF3; CD18; TNFCR; TNFR3; D12S370; TNFR-RP;  TNFR2-RP; LT-BETA-R; TNF-R-III

 

NCBI Gene ID: 4055

 

Uniprot Entry: P36941

 

Construct Details: The recombinant human LTβR-Fc fusion is expressed as a 420 amino acid protein consisting of Gln31 - Thr234 region of LTβR (UniProt accession #P36941) and a C-terminal Fc from human IgG1, which exists as a dimer/tetramer/oligomer under non-reducing conditions (see the gel image inserted above).  

 

Source: Human cells stably expressing human LTβR-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

QAVPPYASEN QTCRDQEKEY YEPQHRICCS RCPPGTYVSA KCSRIRDTVC ATCAENSYNE  

        70         80         90        100        110        120 

HWNYLTICQL CRPCDPVMGL EEIAPCTSKR KTQCRCQPGM FCAAWALECT HCELLSDCPP  

       130        140        150        160        170        180 

GTEAELKDEV GKGNNHCVPC KAGHFQNTSS PSARCQPHTR CENQGLVEAA PGTAQSDTTC 

       190        200        210        220        230        240 

KNPLEPLPPE MSGTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS 

       250        260        270        280        290        300 

HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA 

       310        320        330        340        350        360 

LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP 

       370        380        390        400        410        420 

ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 46.8; Estimated by SDS-PAGE under reducing condition (kDa): 55-60

 

Calculated PI: 6.26

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  57100

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand LIGHT and lymphotoxin-α1/β2. Inhibits LIGHT/lymphotoxin-α1/β2-mediated biological activities including NF-κB activation and cytotoxicity.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Science 264:707 (1994).

2. J. Immunol. 155:5280 (1995).

3. J. Exp. Med. 198:757 (2003).

4. Oncogene 29:5006 (2010).

5. Nat. Rev. Immunol. 10:664 (2010).

6. J. Immunol. 186:5047 (2011).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0098-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.