CD27 is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF7).  CD27 is expressed as a disulfide­linked homodimer on the cell surface. Proteolytic cleavage of CD27 results in the shedding of a 28­32 kDa fragment of the extracellular domain (ECD) with three TNFR cysteine­rich repeats.  The expression of CD27 is limited to cells of the lymphoid lineage.  CD27 is weakly expressed on naïve T cells and NK cells and is up­regulated upon cell activation. It is also up­regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells.  CD27 acts as a co­stimulatory molecule that supports lymphocyte activation and survival. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by a TNF-like ligand CD70 on lymphocytes and dendritic cells. CD27 binds to the transmembrane glycoprotein CD70 that is expressed on activated B cells, activated T cells, and dendritic cells.  CD27 transduces signals and subsequently leads to the activation of NF-κB and JNK pathways that contribute to the activation and survival of T cells and NK cells as well as the long-term maintenance of T cell immunity. CD27 also plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses. Mutations in the CD27 gene are associated with an autosomal recessive immunodeficiency disorder, lymphoproliferative syndrome 2 (LPFS2).

CD27 is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF7).  CD27 is expressed as a disulfide­linked homodimer on the cell surface. Proteolytic cleavage of CD27 results in the shedding of a 28­32 kDa fragment of the extracellular domain (ECD) with three TNFR cysteine­rich repeats.  The expression of CD27 is limited to cells of the lymphoid lineage.  CD27 is weakly expressed on naïve T cells and NK cells and is up­regulated upon cell activation. It is also up­regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells.  CD27 acts as a co­stimulatory molecule that supports lymphocyte activation and survival. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by a TNF-like ligand CD70 on lymphocytes and dendritic cells. CD27 binds to the transmembrane glycoprotein CD70 that is expressed on activated B cells, activated T cells, and dendritic cells.  CD27 transduces signals and subsequently leads to the activation of NF-κB and JNK pathways that contribute to the activation and survival of T cells and NK cells as well as the long-term maintenance of T cell immunity. CD27 also plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses. Mutations in the CD27 gene are associated with an autosomal recessive immunodeficiency disorder, lymphoproliferative syndrome 2 (LPFS2).

  

Product Details

 

Gene Symbol: TNFRSF7; T14; S152; Tp55; S152. LPFS2

  

NCBI Gene ID: 939

 

Uniprot Entry: P26842

 

Construct Details: The recombinant human CD27-Fc fusion is expressed as a 400 amino acid protein consisting of Ala20 - Arg191 region of CD27 (UniProt accession #P26842) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

   

Source: Human cells stably expressing human CD27-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

ATPAPKSCPE RHYWAQGKLC CQMCEPGTFL VKDCDQHRKA AQCDPCIPGV SFSPDHHTRP 

        70         80         90        100        110        120 

HCESCRHCNS GLLVRNCTIT ANAECACRNG WQCRDKECTE CDPLPNPSLT ARSSQALSPH 

       130        140        150        160        170        180 

PQPTHLPYVS EMLEARTAGH MQTLADFRQL PARTLSTHWP PQRSLCSSDF IRSTGTHTCP 

       190        200        210        220        230        240 

PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA 

       250        260        270        280        290        300 

KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ 

       310        320        330        340        350        360 

VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY 

       370        380        390        400 

SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 44.8; Estimated by SDS-PAGE under reducing condition (kDa): 65-70

 

Calculated PI: 7.81

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 56265

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand CD70 and inhibits CD70-mediated signaling activities including proliferation of  T cells induced by CD70 and anti­CD3 antibody. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. J. Immunol. 139:1589 (1987). 

2. Cell 73:447 (1993).

3. J. Immunol. 152:1756 (1994).

4. Proc. Natl. Acad. Sci. 92: 11249 (1995). 

5. Immunol. Rev. 229:216 (2009).

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0102-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.