The mannose 6-phophate receptors (MPRs) are single-pass type I transmembrane glycoproteins that target the newly synthesized hydrolases from the Golgi complex and the cell surface to lysosomes. There are two different MPRs, one of ~300kDa termed CI-MPR (cation-independent) and a smaller, dimeric receptor of ~46kDa termed CD-MPR (cation-depnendent). Both MPRss are P-type lectins that bind terminal mannose 6-phosphate (M6P) with similar affinity (CI-MPR = 7 μM, CD-MPR = 8 μM) and contain similar cytoplasmic signals for intracellular trafficking. CD-MPR shows greatly enhanced binding to M6P in the presence of divalent cations, such as manganese. The MPRs, when bound to their cargo via M6P, are recognized by the GGA family of clathrin adaptor proteins and accumulate in forming clathrin-coated vesicles. The vesicles are trafficked to the early endosome where, in the relatively low pH environment of the endosome, the MPRs release their cargo. The cargo proteins are then trafficked to the lysosome via the late endosome independently of the MPRs. CD-MPR is also known as M6PR that contain a 157 amino-acid M6P-binding-domain in the extracellular/luminal region. CI-MPR was also identified as the cell surface receptor for insulin-like growth factor 2 (IGF2R/CD222). IGF2R consists of 15 repeats homologous to the M6P-binding domain present in M6PR/CD-MPR and two of these 15 repeats are responsible for binding to M6P. 

The mannose 6-phophate receptors (MPRs) are single-pass type I transmembrane glycoproteins that target the newly synthesized hydrolases from the Golgi complex and the cell surface to lysosomes. There are two different MPRs, one of ~300kDa termed CI-MPR (cation-independent) and a smaller, dimeric receptor of ~46kDa termed CD-MPR (cation-depnendent). Both MPRss are P-type lectins that bind terminal mannose 6-phosphate (M6P) with similar affinity (CI-MPR = 7 μM, CD-MPR = 8 μM) and contain similar cytoplasmic signals for intracellular trafficking. CD-MPR shows greatly enhanced binding to M6P in the presence of divalent cations, such as manganese. The MPRs, when bound to their cargo via M6P, are recognized by the GGA family of clathrin adaptor proteins and accumulate in forming clathrin-coated vesicles. The vesicles are trafficked to the early endosome where, in the relatively low pH environment of the endosome, the MPRs release their cargo. The cargo proteins are then trafficked to the lysosome via the late endosome independently of the MPRs. CD-MPR is also known as M6PR that contain a 157 amino-acid M6P-binding-domain in the extracellular/luminal region. CI-MPR was also identified as the cell surface receptor for insulin-like growth factor 2 (IGF2R/CD222). IGF2R consists of 15 repeats homologous to the M6P-binding domain present in M6PR/CD-MPR and two of these 15 repeats are responsible for binding to M6P. 

 

Product Details

 

Gene Symbol: M6PR; SMPR; MPR46; CD-MPR; MPR 46; MPR-46; MPRD

 

NCBI Gene ID: 4074

 

Uniprot Entry: P20645

 

Construct Details: The recombinant human M6PR-Fc is expressed as a 387 amino acid protein consisting of Thr27 - Glu182 region of M6PR (UniProt #P20645) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

 

Source:  Human cells stably expressing human M6PR-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

TEEKTCDLVG EKGKESEKEL ALVKRLKPLF NKSFESTVGQ GSDTYIYIFR VCREAGNHTS 

        70         80         90        100        110        120 

GAGLVQINKS NGKETVVGRL NETHIFNGSN WIMLIYKGGD EYDNHCGKEQ RRAVVMISCN 

       130        140        150        160        170        180 

RHTLADNFNP VSEERGKVQD CFYLFEMDSS LACSPESTGS TGTHTCPPCP APELLGGPSV 

       190        200        210        220        230        240 

FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY 

       250        260        270        280        290        300 

RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK 

       310        320        330        340        350        360 

NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG 

       370        380 

NVFSCSVMHE ALHNHYTQKS LSLSPGK 

  

M.W.:  Calculated molecular mass 43.3 kDa; estimated by SDS-PAGE under reducing condition 55-60 kDa probably due to glycosylation

 

Calculated PI: 6.42

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 49110

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

  

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 82: 4428 (1985).

2. J. Biol. Chem. 260: 12008 (1985).

3. Proc. Natl. Acad. Sci. 84:5575 (1987). 

4. J. Biol. Chem. 264: 7970 (1989).

5. Science 292:1712 (2001).

6. J. Biol. Chem. 277:18477 (2002). 

 

 

 

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0131-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.