Human CDNF, full-length gene in lentivector, endotoxin-free DNA

CDNF (conserved dopamine neurotrophic factor) is also known as ARMETL1 (arginine­rich, mutated in early stage tumors­like 1) in mouse.  Mature CDNF protein consists of 161 amino acid residues (its precursor has 187 amino acids), and shares 62% amino acid (aa) identity with human MANF (mesencephalic­ astrocyte­derived neurotrophic factor), termed ARMET in mouse.  CDNF was first identified by bioinformatics and then biochemically characterized.  Vertebrates appear to have CDNF and MANF genes, whereas invertebrates have a single homologous gene more closely related to MANF than to CDNF.  Mature human CDNF shares 80%, 84%, 90% and 92% aa identity with mouse, rat, equine and bovine CDNF, respectively.  Human CDNF contains one potential N-linked glycosylation site, and both unglycosylated and glycosylated form of human CDNF is secreted from transiently overexpressing cells.  Both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfides, and a C-­terminal endoplasmic reticulum retention signal.  The CDNF gene is widely expressed in neuronal and non-neuronal tissues.  In the brain, highest levels in the optic nerve and corpus callosum. Although CDNF mRNA and protein are expressed in pre­ and post­natal mouse brain, they are most abundant in adult heart, skeletal muscle and testis.  Similar to MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro. In a rat Parkinson’s disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo.

Gene Symbol: CDNF; ARMETL1

NCBI Gene ID: 441549

Uniprot Entry: Q49AH0

Construct Details: Full length human CDNF gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 564 (bp)

Gene Insert Sequence: 

ATGTGGTGCGCGAGCCCAGTTGCTGTGGTGGCCTTTTGCGCCGGGCTTTTGGTCTCTCACCCGGTGCTGACGCAGGGCCA
GGAGGCCGGGGGGCGGCCAGGGGCCGACTGTGAAGTATGTAAAGAATTCTTGAACCGATTCTACAAGTCACTGATAGACA
GAGGAGTTAACTTTTCGCTGGACACTATAGAGAAAGAATTGATCAGTTTTTGCTTGGACACCAAAGGAAAAGAAAACCGC
CTGTGCTATTATCTAGGAGCCACAAAAGACGCAGCCACAAAGATCCTAAGTGAAGTCACTCGCCCAATGAGTGTGCATAT
GCCTGCAATGAAGATTTGTGAGAAGCTGAAGAAGTTGGATAGCCAGATCTGTGAGCTGAAATATGAAAAAACACTGGACT
TGGCATCAGTTGACCTGCGGAAGATGAGAGTGGCAGAGCTGAAGCAGATCCTGCATAGCTGGGGGGAGGAGTGCAGGGCC
TGTGCAGAAAAAACTGACTATGTGAATCTCATTCAAGAGCTGGCCCCCAAGTATGCAGCGACACACCCCAAAACAGAGCT
CTGA

Primers: Gene insert coding sequence confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C.  Upon receipt, centrifuge the vial briefly before opening.  Store at –20°C for long-term storage and the product is stable for 6 months. 

Avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMAN. NOT RESELLABLE OR TRANSFERABLE

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

1.  Develop Neurobiol 70: 360, 2010
2.  Nature 448:73, 2007
3.  Cell Struct. Funct 32:41, 2007
4.   J Cell Biol 179:1193, 2007

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Product datasheet (pdf) can be downloaded here: LTP0288-PDS.pdf

Additional supporting documents, including plasmid map & sequence, COA and MSDS are available upon request.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.