B7­H2 (B7 homolog 2), also known as CD275, B7RP1 (B7­related protein) and ICOSL (ICOS Ligand), is a single-pass type I transmembrane glycoprotein that belongs to the B7 family of the immunoglobulin (Ig) superfamily.  Like other B7 family members, B7-H25 contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells. B7-H2 has been identified as the ligand for ICOS (also known as CD278), a member of the CD28/CTLA4 family of co-stimulatory receptors. The receptor binding of B7-H2 is mediated by the IgV domain and requires the IgC domain for maintaining the structural integrity of the protein.  The B7-H2/ICOS interaction plays roles in T cell dependent B cell activation and Th (T helper) cell differentiation, leading to both positive and negative effects on immune responses. In human, B7-H2 also binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate T cells. B7-H2 contributes to the development of allergic asthma and other autoimmune conditions. A soluble form of human B7­H2 is elevated in the circulation of patients with systemic lupus erythematosus. In the thyroid, B7­H2 is up­regulated on thyrocytes during inflammation and promotes the production of thryoid hormones.  It is reported that mouse and human B7-H2 show cross­species binding to ICOS.

B7­H2 (B7 homolog 2), also known as CD275, B7RP1 (B7­related protein) and ICOSL (ICOS Ligand), is a single-pass type I transmembrane glycoprotein that belongs to the B7 family of the immunoglobulin (Ig) superfamily.  Like other B7 family members, B7-H25 contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells. B7-H2 has been identified as the ligand for ICOS (also known as CD278), a member of the CD28/CTLA4 family of co-stimulatory receptors. The receptor binding of B7-H2 is mediated by the IgV domain and requires the IgC domain for maintaining the structural integrity of the protein.  The B7-H2/ICOS interaction plays roles in T cell dependent B cell activation and Th (T helper) cell differentiation, leading to both positive and negative effects on immune responses. In human, B7-H2 also binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate T cells. B7-H2 contributes to the development of allergic asthma and other autoimmune conditions. A soluble form of human B7­H2 is elevated in the circulation of patients with systemic lupus erythematosus. In the thyroid, B7­H2 is up­regulated on thyrocytes during inflammation and promotes the production of thryoid hormones.  It is reported that mouse and human B7-H2 show cross­species binding to ICOS.

 

Product Details

 

Gene Symbol: B7-H2; CD275; ICOSL; B7H2; GL50; ICOSLG; B7RP1; ICOS-L; LICOS; B7RP-1; KIAA0653

 

NCBI Gene ID: 23308

 

Uniprot Entry: O75144

 

Construct Details: The recombinant human B7-H2/ICOSL/CD275 ECD is expressed as a 249 amino acid protein consisting of Asp19 - Thr256 region of B7-H2 (UniProt accession #O75144) and a C-terminal His-tag. It contains 6 potential sites for N-linked glycosylation.

 

Source:  Human cells stably expressing B7-H2 ECD and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGD

FSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWI

NKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVST

GEKNAATSTGHHHHHHHH

 

M.W.:  Calculated molecular mass 27.8 kDa; estimated by SDS-PAGE under reducing condition 50-60 kDa probably due to glycosylation

 

Calculated PI: 5.88

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 21805

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds human ICOS and stimulates human T cell proliferation in the presence of anti-CD3.

  

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Nature 402:827 (1999). 

2. Blood 96:2808 (2000). 

3. Nat. Med. 8:1024 (2002). 

4. J. Immunol. 174:3000 (2005). 

5. Immunity 32:729 (2011). 

 

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0866-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.