DcR3 (death decoy receptor), also known as decoy receptor for Fas ligand, TR6 and M68, is a member of the TNF receptor superfamily (TNFRSF6B).  Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein that contains 4 TNFR cysteine-rich repeats. Expression of DcR3 has been reported in a wide array of normal tissues as well as primary tumors and tumor cell lines. DcR3 is detected in fetal lung, brain and liver and in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung.  DcR3 is highly expressed in primary tumors from colon, stomach, rectum, and esophagus as well as in SW480 colon cancer cell line. DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and TNF- like molecule 1A (TL1A). The principal function of DcR3 is the inhibition of FasL­ and LIGHT-­induced apoptosis. In addition to DcR1, DcR2 and OPG, DcR3 is another TNFRSF member that modulates cytotoxic ligands-induced apoptosis. Overexpression of DcR3 may be a mechanism by which certain tumors escape immune­cytotoxic attack. DcR3 may also modulate CNS- autoimmunity by interfering with Th17 responses via the blockade of Fas-FasL interaction.

DcR3 (death decoy receptor), also known as decoy receptor for Fas ligand, TR6 and M68, is a member of the TNF receptor superfamily (TNFRSF6B).  Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein that contains 4 TNFR cysteine-rich repeats. Expression of DcR3 has been reported in a wide array of normal tissues as well as primary tumors and tumor cell lines. DcR3 is detected in fetal lung, brain and liver and in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung.  DcR3 is highly expressed in primary tumors from colon, stomach, rectum, and esophagus as well as in SW480 colon cancer cell line. DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and TNF- like molecule 1A (TL1A). The principal function of DcR3 is the inhibition of FasL­ and LIGHT-­induced apoptosis. In addition to DcR1, DcR2 and OPG, DcR3 is another TNFRSF member that modulates cytotoxic ligands-induced apoptosis. Overexpression of DcR3 may be a mechanism by which certain tumors escape immune­cytotoxic attack. DcR3 may also modulate CNS- autoimmunity by interfering with Th17 responses via the blockade of Fas-FasL interaction.

  

Product Details

 

Gene Symbol: TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1; UNQ186/PRO212

  

NCBI Gene ID: 8771

 

Uniprot Entry: Q95407

 

Construct Details: The recombinant human DcR3-Fc fusion is expressed as a 499 amino acid protein consisting of Val30 - His300 region of DcR3 (UniProt accession #O95407) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

 

Source: Human cells stably expressing human DcR3-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

VAETPTYPWR DAETGERLVC AQCPPGTFVQ RPCRRDSPTT CGPCPPRHYT QFWNYLERCR 

        70         80         90        100        110        120 

YCNVLCGERE EEARACHATH NRACRCRTGF FAHAGFCLEH ASCPPGAGVI APGTPSQNTQ 

       130        140        150        160        170        180 

CQPCPPGTFS ASSSSSEQCQ PHRNCTALGL ALNVPGSSSH DTLCTSCTGF PLSTRVPGAE 

       190        200        210        220        230        240 

ECERAVIDFV AFQDISIKRL QRLLQALEAP EGWGPTPRAG RAALQLKLRR RLTELLGAQD 

       250        260        270        280        290        300 

GALLVRLLQA LRVARMPGLE RSVRERFLPV HSTGTHTCPP CPAPELLGGP SVFLFPPKPK 

       310        320        330        340        350        360 

DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV 

       370        380        390        400        410        420 

LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL 

       430        440        450        460        470        480 

VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM 

       490 

HEALHNHYTQ KSLSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 53.3; Estimated by SDS-PAGE under reducing condition (kDa): ~60

 

Calculated PI: 8.41

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 59495

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to human FasL and Light.  Inhibits FasL-­induced apoptosis in human Jurkat acute T cell leukemia cells.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Nature 396:699 (1998).

2. J. Biochem. Chem. 274:13733 (1999).

3. Proc. Natl. Acad. Sci. 97:1230 (2000).

4. J Am Soc Nephrol. 19: 1025 (2008). 

5. J Neuroimmunol. 209: 57 (2009).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCLSF55B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.