CD90, also known as Thy1, is a GPI-anchored cell membrane protein that contains one immunoglobulin (Ig)-like V-type (IgV) domain in the extracellular region.  Cd90 is thus a member of the Ig superfamily that mediates cellular adhesion and exerts a wide range effects in different tissues.  CD90 was originally identified on mouse thymocytes as a thymocyte and pan T cell marker in mouse but not in human. CD90 is also found on various stem cells and non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. It is expressed on hematopoietic progenitor cells, neurons, and activated vascular endothelial cells.  CD90 can form dimers and higher order multimers. CD90 binds to heparin, the proteoglycan Syndecan­4, and Integrins including αMβ2, αVβ3, and αVβ5.  Through these interactions, CD90 inhibits neurite outgrowth, promotes astrocyte adhesion, mediates the extravasation of leukocytes and melanoma cells, supports the Thrombospondin­1 induced disassembly of fibroblast focal adhesions, and inhibits the activation of myofibroblast differentiation and lung fibrosis. CD90 may be a prognostic marker for Neuroblastoma (NBL) which is the most common solid tumor in children. CD90 may also play an important role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the central nervous system.

CD90, also known as Thy1, is a GPI-anchored cell membrane protein that contains one immunoglobulin (Ig)-like V-type (IgV) domain in the extracellular region.  Cd90 is thus a member of the Ig superfamily that mediates cellular adhesion and exerts a wide range effects in different tissues.  CD90 was originally identified on mouse thymocytes as a thymocyte and pan T cell marker in mouse but not in human. CD90 is also found on various stem cells and non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. It is expressed on hematopoietic progenitor cells, neurons, and activated vascular endothelial cells.  CD90 can form dimers and higher order multimers. CD90 binds to heparin, the proteoglycan Syndecan­4, and Integrins including αMβ2, αVβ3, and αVβ5.  Through these interactions, CD90 inhibits neurite outgrowth, promotes astrocyte adhesion, mediates the extravasation of leukocytes and melanoma cells, supports the Thrombospondin­1 induced disassembly of fibroblast focal adhesions, and inhibits the activation of myofibroblast differentiation and lung fibrosis. CD90 may be a prognostic marker for Neuroblastoma (NBL) which is the most common solid tumor in children. CD90 may also play an important role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the central nervous system.

 

Product Details

 

Gene Symbol: CD90; THY1; CDw90

 

NCBI Gene ID: 7070

 

Uniprot Entry: P04216

 

Construct Details: The recombinant human CD90-Fc fusion is expressed as a 348 amino acid protein consisting of Gln20 - Lys129 region of CD90 (Uniprot Accession #P04216) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD90-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

QKVTSLTACL VDQSLRLDCR HENTSSSPIQ YEFSLTRETK KHVLFGTVGV PEHTYRSRTN 

        70         80         90        100        110        120 

FTSKYNMKVL YLSAFTSKDE GTYTCALHHS GHSPPISSQN VTVLRDKLVK STTENLYFQG 

       130        140        150        160        170        180 

STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV 

       190        200        210        220        230        240 

DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA 

       250        260        270        280        290        300 

KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD 

       310        320        330        340 

SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 39.2; Estimated by SDS-PAGE under reducing condition (kDa): 55-60

 

Calculated PI:  8.45 

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 44850

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Immobilized CD90-Fc protein supports the adhesion of human neutrophils with an ED₅₀ typically between 0.5 ­ 2.5 μg/mL

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 82:3819 (1985). 

2. Nature 355:745 (1992)

3. J. Exp. Med. 177:1331 (1993). 

4. J. Immunol. 173:3581 (2004).

5. Oncogene 24:4710 (2010).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2186B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.