CD146, also known as MUC18 and MCAM, is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. CD146 contains 2 Ig-like V-type domains and 3 Ig-like C2-type domains (V-V-C2-C2-C2) in the extracellular region (ECD) in addition to a transmembrane (TM) region and a short cytoplasmic tail. CD146 is expressed in endothelial cells in vascular tissue throughout the body. Its expression appears to be limited to vascular smooth muscle in normal adult tissues. CD146 is involved in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 is also an adhesion molecule active in neural crest cells during embryonic development. CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. CD146 can act as a cell surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1 as well as a transient increase in the intracellular calcium concentration. CD146 is expressed most strongly on metastatic lesions and advanced primary tumors while it is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.  It thus suggests that CD146 may play an important role in tumor progression and metastasis.

CD146, also known as MUC18 and MCAM, is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. CD146 contains 2 Ig-like V-type domains and 3 Ig-like C2-type domains (V-V-C2-C2-C2) in the extracellular region (ECD) in addition to a transmembrane (TM) region and a short cytoplasmic tail. CD146 is expressed in endothelial cells in vascular tissue throughout the body. Its expression appears to be limited to vascular smooth muscle in normal adult tissues. CD146 is involved in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 is also an adhesion molecule active in neural crest cells during embryonic development. CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. CD146 can act as a cell surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1 as well as a transient increase in the intracellular calcium concentration. CD146 is expressed most strongly on metastatic lesions and advanced primary tumors while it is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.  It thus suggests that CD146 may play an important role in tumor progression and metastasis.

 

Product Details

 

Gene Symbol: CD146; MUC18; MCAM; P1H12; A32

 

NCBI Gene ID: 4162

 

Uniprot Entry: P43121

 

Construct Details: The recombinant human CD146-Fc fusion is expressed as a 774 amino acid protein consisting of Val24 - Gly559 region of CD146 (Uniprot Accession #P43121) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD146-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

VPGEAEQPAP ELVEVEVGST ALLKCGLSQS QGNLSHVDWF SVHKEKRTLI FRVRQGQGQS 

        70         80         90        100        110        120 

EPGEYEQRLS LQDRGATLAL TQVTPQDERI FLCQGKRPRS QEYRIQLRVY KAPEEPNIQV 

       130        140        150        160        170        180 

NPLGIPVNSK EPEEVATCVG RNGYPIPQVI WYKNGRPLKE EKNRVHIQSS QTVESSGLYT 

       190        200        210        220        230        240 

LQSILKAQLV KEDKDAQFYC ELNYRLPSGN HMKESREVTV PVFYPTEKVW LEVEPVGMLK 

       250        260        270        280        290        300 

EGDRVEIRCL ADGNPPPHFS ISKQNPSTRE AEEETTNDNG VLVLEPARKE HSGRYECQGL 

       310        320        330        340        350        360 

DLDTMISLLS EPQELLVNYV SDVRVSPAAP ERQEGSSLTL TCEAESSQDL EFQWLREETG 

       370        380        390        400        410        420 

QVLERGPVLQ LHDLKREAGG GYRCVASVPS IPGLNRTQLV NVAIFGPPWM AFKERKVWVK 

       430        440        450        460        470        480 

ENMVLNLSCE ASGHPRPTIS WNVNGTASEQ DQDPQRVLST LNVLVTPELL ETGVECTASN 

       490        500        510        520        530        540 

DLGKNTSILF LELVNLTTLT PDSNTTTGLS TSTASPHTRA NSTSTERKLP EPESRGSTTE 

       550        560        570        580        590        600 

NLYFQGSTGT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV  

       610        620        630        640        650        660 

KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE 

       670        680        690        700        710        720 

KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT 

       730        740        750        760        770 

TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK 

 

M.W.: Calculated molecular mass (kDa): 86.4; Estimated by SDS-PAGE under reducing condition (kDa): 90-100

 

Calculated PI: 5.50 

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 94280

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Immobilized CD146-Fc protein supports the adhesion of HUVEC (human umbilical vein endothelial cell)

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 86:9891 (1988)

2. Melanoma Res. 3:337 (1992)

3. J. Biol. Chem. 276:1564 (2000) 

4. Cancer Res. 54:2514 (1993)

5. J. Immunol. 179: 6673 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2193B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.