CEA (carcinoembryonic antigen), also kown as CEACAM5, BGP1 and CD66e, is a member of the CEA gene family belonging to the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI­anchored within two subgroups: the CEA cell adhesion molecules (CEACAM) and the pregnancy-specific glycoproteins.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEA or CEACAM-5 contains1 Ig-like V-type (IgV) domain at the N-terminus, followed by 6 Ig-like C2-type (IgC2) domain and a GPI anchor.  It exists as a homodimer . Intriguingly orthologs of human CEA have not been found in other species. Human CEA is expressed primarily in epithelial cells and functions as an adhesion molecule through homophilic and heterophilic interactions with CEACAM1/CD66a. CEA plays an important role in the development and maintenance of epithelial architecture. CEA is up-regulated in a wide array of human tumors and has been used a s a common cancer biomarker. CEA can promote tumor cell migration, invasion, adhesion, and metastasis.  It may also contribute to tumorigenicity by inhibiting cellular differentiation. GPI-anchored CEA can be released by GPI­PLD, resulting in a soluble molecule that induces tumor metastasis.  CEA has been considered as a a target for immunotherapy. 

CEA (carcinoembryonic antigen), also kown as CEACAM5, BGP1 and CD66e, is a member of the CEA gene family belonging to the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI­anchored within two subgroups: the CEA cell adhesion molecules (CEACAM) and the pregnancy-specific glycoproteins.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEA or CEACAM-5 contains1 Ig-like V-type (IgV) domain at the N-terminus, followed by 6 Ig-like C2-type (IgC2) domain and a GPI anchor.  It exists as a homodimer . Intriguingly orthologs of human CEA have not been found in other species. Human CEA is expressed primarily in epithelial cells and functions as an adhesion molecule through homophilic and heterophilic interactions with CEACAM1/CD66a. CEA plays an important role in the development and maintenance of epithelial architecture. CEA is up-regulated in a wide array of human tumors and has been used a s a common cancer biomarker. CEA can promote tumor cell migration, invasion, adhesion, and metastasis.  It may also contribute to tumorigenicity by inhibiting cellular differentiation. GPI-anchored CEA can be released by GPI­PLD, resulting in a soluble molecule that induces tumor metastasis.  CEA has been considered as a a target for immunotherapy. 

 

Product Details

 

Gene Symbol: CEA; CEACAM5; CEACAM-5; CD66e; CEAM5

 

NCBI Gene ID: 1048

 

Uniprot Entry: P06731

 

Construct Details: The recombinant human CEA-Fc fusion is expressed as a 886 amino acid protein consisting of Lys35 - Gly682 region of CEA (Uniprot Accession #P06731) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CEA-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

KLTIESTPFN VAEGKEVLLL VHNLPQHLFG YSWYKGERVD GNRQIIGYVI GTQQATPGPA  

        70         80         90        100        110        120 

YSGREIIYPN ASLLIQNIIQ NDTGFYTLHV IKSDLVNEEA TGQFRVYPEL PKPSISSNNS 

       130        140        150        160        170        180 

KPVEDKDAVA FTCEPETQDA TYLWWVNNQS LPVSPRLQLS NGNRTLTLFN VTRNDTASYK  

       190        200        210        220        230        240 

CETQNPVSAR RSDSVILNVL YGPDAPTISP LNTSYRSGEN LNLSCHAASN PPAQYSWFVN 

       250        260        270        280        290        300 

GTFQQSTQEL FIPNITVNNS GSYTCQAHNS DTGLNRTTVT TITVYAEPPK PFITSNNSNP 

       310        320        330        340        350        360 

VEDEDAVALT CEPEIQNTTY LWWVNNQSLP VSPRLQLSND NRTLTLLSVT RNDVGPYECG 

       370        380        390        400        410        420 

IQNKLSVDHS DPVILNVLYG PDDPTISPSY TYYRPGVNLS LSCHAASNPP AQYSWLIDGN  

       430        440        450        460        470        480 

IQQHTQELFI SNITEKNSGL YTCQANNSAS GHSRTTVKTI TVSAELPKPS ISSNNSKPVE 

       490        500        510        520        530        540 

DKDAVAFTCE PEAQNTTYLW WVNGQSLPVS PRLQLSNGNR TLTLFNVTRN DARAYVCGIQ 

       550        560        570        580        590        600 

NSVSANRSDP VTLDVLYGPD TPIISPPDSS YLSGANLNLS CHSASNPSPQ YSWRINGIPQ 

       610        620        630        640        650        660 

QHTQVLFIAK ITPNNNGTYA CFVSNLATGR NNSIVKSITV SASGTSPGST TENLYFQGST 

       670        680        690        700        710        720 

GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG 

       730        740        750        760        770        780 

VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG 

       790        800        810        820        830        840 

QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD 

       850        860        870        880 

GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK 

 

M.W.: Calculated molecular mass (kDa): 98; Estimated by SDS-PAGE under reducing condition (kDa): 80-90, 110-120, and 140-150.

 

Calculated PI: 5.82

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 134745

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds human Galectin­-4 and CEACAM1/5 via a homophilic and heterophilic interaction. Blocks CEA-mediated cell-cell adhesion and cell migration.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 85:4648 (1988).

2. Mol. Cell. Biol. 10: 2738 (1990).

3. Cancer Res. 51:5679 (1991).

4. J. Exp. Med. 185:1557 (1997).

5. Semin. Cancer Biol. 9:67 (1999).

6. J. Biol. Chem. 275: 26935 (2000).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2139-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.