CD47, also known as IAP and OA3, is a variably glycosylated transmembrane protein of the immunoglobulin superfamily (IgSF). It contains an extracellular domain (ECD) with a single Ig-like domain, five transmembrane domains with short intervening loops and a 34 amino acid cytoplasmic tail at the C-terminus. CD47 is broadly expressed. It is involved in the increase in intracellular calcium concentration in response to cell adhesion to extracellular matrix. By acting as an adhesion receptor, CD47 plays a role in both cell adhesion by positively or negatively modulating Integrin-mediated function. CD47 also binds to the SIRP family members on macrophages, neutrophils, and T lymphocytes, preventing macrophage­mediated clearance of CD47­expressing cells and promoting immune cell transmigration across the vascular endothelium.  CD47 binds SIRPA which prevents maturation of dendritic cells and inhibits cytokine production. CD47 also interacts with SIRPG that enhances superantigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation. CD47 is also a receptor for thrombospondin, and inhibits the angiogenic and vasorelaxant effects in the vasculature. On dendritic cells and myeloma cells, CD47 binds TSP-1 and induces giant cell formation and osteoclast differentiation.

CD47, also known as IAP and OA3, is a variably glycosylated transmembrane protein of the immunoglobulin superfamily (IgSF). It contains an extracellular domain (ECD) with a single Ig-like domain, five transmembrane domains with short intervening loops and a 34 amino acid cytoplasmic tail at the C-terminus. CD47 is broadly expressed. It is involved in the increase in intracellular calcium concentration in response to cell adhesion to extracellular matrix. By acting as an adhesion receptor, CD47 plays a role in both cell adhesion by positively or negatively modulating Integrin-mediated function. CD47 also binds to the SIRP family members on macrophages, neutrophils, and T lymphocytes, preventing macrophage­mediated clearance of CD47­expressing cells and promoting immune cell transmigration across the vascular endothelium.  CD47 binds SIRPA which prevents maturation of dendritic cells and inhibits cytokine production. CD47 also interacts with SIRPG that enhances superantigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation. CD47 is also a receptor for thrombospondin, and inhibits the angiogenic and vasorelaxant effects in the vasculature. On dendritic cells and myeloma cells, CD47 binds TSP-1 and induces giant cell formation and osteoclast differentiation.

 

Product Details

 

Gene Symbol: CD47; IAP; OA3; MER6

 

NCBI Gene ID: 961

 

Uniprot Entry: Q08722

 

Construct Details: The recombinant human CD47-Fc fusion is expressed as a 361 amino acid protein consisting of Gln19 - Asp141 region of CD47 (Uniprot Accession #Q08722) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD47-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP 

        70         80         90        100        110        120 

TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS 

       130        140        150        160        170        180 

PNESTTENLY FQGSTGTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV 

       190        200        210        220        230        240 

SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK 

       250        260        270        280        290        300 

ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ 

       310        320        330        340        350        360 

PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG 

 

K

 

M.W.: Calculated molecular mass (kDa): 41; Estimated by SDS-PAGE under reducing condition (kDa): 60-65

 

Calculated PI: 6.12

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 54360

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds human SIRPA and SIRPG proteins.  Blocks red blood cell adhesion to immobilized SIRPA protein.  Inhibits CD47-mediated signaling activity.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cancer Res. 52:5416 (1992).

2. Science 288:2051 (2000).

3. Trends Cell Biol. 11: 130 (2001).

4. J. Immunol. 168:5832 (2002).

5. Blood 109:836 (2007).

6. Curr. Drug Targ. 9:842 (2009).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2233B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.