Human CSF1/MCSF Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Product ID: CSF1-Fc (SKU#: FCL2669)

Price:

$598.00

Description

CSF1 (colony stimulating factor 1), also known as MCSF (macrophage colony stimulating factor) is a single-pass type I transmembrane or secreted protein. The active form of CSF1 is a disulfide-linked homodimer, produced by proteolytic cleavage of membrane-bound precursors. As a cytokine, CSF1 controls the production, differentiation, and function of macrophages. CSF1 primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. CSF1 induces the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. CSF1 is required for male and female fertility. CSF1 induces reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Aberrant expression of CSF1 or its receptor CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in many types of cancers including breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R is implicated in inflammatory conditions, such as rheumatoid arthritis (RA), glomerulonephritis, atherosclerosis, and allograft rejection.

Gene Symbol: CSF1; MCSF; CSF-1; M-CSF

NCBI Gene ID: 1435

Uniprot Entry: P09603

Construct Details: The recombinant human CSF1-Fc is expressed as a 388-amino acid protein consisting of Glu33 - Gln181 region of CSF1 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.

Source: Human cells stably expressing human CSF1-Fc and growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

10 20 30 40 50 60

EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI

70 80 90 100 110 120

MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV

130 140 150 160 170 180

FNETKNLLDK DWNIFSKNCN NSFAECSSQG STTENLYFQG STGTHTCPPC PAPELLGGPS

190 200 210 220 230 240

VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST

250 260 270 280 290 300

YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT

310 320 330 340 350 360

KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ

370 380

GNVFSCSVMH EALHNHYTQK SLSLSPGK

M.W.: Calculated molecular mass (kDa): 44.2 Estimated by SDS-PAGE under reducing condition (kDa): 50-55.

Calculated PI: 5.66

Calculated extinction coefficients (M-1 cm-1, at 280nm): 49110

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Binds its receptor human CSF1R (SKU#FCL2648) and induces CSF1R-mediated signaling activity including proliferation of leukemia lymphoblast cells.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. Science 230:291-296(1985)

2. Science 235:1504-1508(1987)

3. Oncogene Res. 4:9-17(1989)

4. Curr. Opin. Immunol. 18:39-48(2006)

5. Cytokine 52:215-220(2010)

Product datasheet (pdf) can be downloaded here: FCL2669-PDS.pdf