DLL1 (Delta-like protein 1) is a single pass type I transmembrane protein of the delta/serrate/jagged family. DLL1 is a human homolog of the Notch Delta ligand and can bind and activate NOTCH1, NOTCH2 and NOTCH3 receptors.  DLL1 contains 1 DSL (delta serrate ligand) domain and 8 EGF-like domains in the extracellular region. The DSL domain is required for binding to the Notch receptors.  The EGF-like domain is often found in tandem repeat arrangements present in a large number of membrane-bound and extracellular proteins. Many of these proteins require calcium for their biological function and calcium-binding may be crucial for numerous protein-protein interactions. Six conserved core cysteines form three disulfide bridges as in non calcium-binding EGF domains with similar structures. DLL1 plays a role in mediating cell fate decisions during hematopoiesis and in cell-to-cell communication. DLL1 is required for embryonic development and maintenance of adult stem cells in many different tissues and immune systeme. The DLL1-induced Notch signaling is mediated through an intercellular communication that regulates cell lineage, cell specification, cell patterning and morphogenesis through effects on differentiation and proliferation.  DLL1 plays a role in brain development at different level, namely by regulating neuronal differentiation of neural precursor cells via cell-cell interaction. DLL1 also may play a role during muscle development. 

DLL1 (Delta-like protein 1) is a single pass type I transmembrane protein of the delta/serrate/jagged family. DLL1 is a human homolog of the Notch Delta ligand and can bind and activate NOTCH1, NOTCH2 and NOTCH3 receptors.  DLL1 contains 1 DSL (delta serrate ligand) domain and 8 EGF-like domains in the extracellular region. The DSL domain is required for binding to the Notch receptors.  The EGF-like domain is often found in tandem repeat arrangements present in a large number of membrane-bound and extracellular proteins. Many of these proteins require calcium for their biological function and calcium-binding may be crucial for numerous protein-protein interactions. Six conserved core cysteines form three disulfide bridges as in non calcium-binding EGF domains with similar structures. DLL1 plays a role in mediating cell fate decisions during hematopoiesis and in cell-to-cell communication. DLL1 is required for embryonic development and maintenance of adult stem cells in many different tissues and immune systeme. The DLL1-induced Notch signaling is mediated through an intercellular communication that regulates cell lineage, cell specification, cell patterning and morphogenesis through effects on differentiation and proliferation.  DLL1 plays a role in brain development at different level, namely by regulating neuronal differentiation of neural precursor cells via cell-cell interaction. DLL1 also may play a role during muscle development. 

 

Product Details

 

Gene Symbol: DLL1; Delta1; Delta; H-Delta-1; UNQ146/PRO172

 

NCBI Gene ID: 28514

 

Uniprot Entry: O00548

 

Construct Details: Full length human DLL1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2172 (bp)

 

Gene Insert Sequence:  

ATGGGCAGTCGGTGCGCGCTGGCCCTGGCGGTGCTCTCGGCCTTGCTGTGTCAGGTCTGGAGCTCTGGGG

TGTTCGAACTGAAGCTGCAGGAGTTCGTCAACAAGAAGGGGCTGCTGGGGAACCGCAACTGCTGCCGCGG

GGGCGCGGGGCCACCGCCGTGCGCCTGCCGGACCTTCTTCCGCGTGTGCCTCAAGCACTACCAGGCCAGC

GTGTCCCCCGAGCCGCCCTGCACCTACGGCAGCGCCGTCACCCCCGTGCTGGGCGTCGACTCCTTCAGTC

TGCCCGACGGCGGGGGCGCCGACTCCGCGTTCAGCAACCCCATCCGCTTCCCCTTCGGCTTCACCTGGCC

GGGCACCTTCTCTCTGATTATTGAAGCTCTCCACACAGATTCTCCTGATGACCTCGCAACAGAAAACCCA

GAAAGACTCATCAGCCGCCTGGCCACCCAGAGGCACCTGACGGTGGGCGAGGAGTGGTCCCAGGACCTGC

ACAGCAGCGGCCGCACGGACCTCAAGTACTCCTACCGCTTCGTGTGTGACGAACACTACTACGGAGAGGG

CTGCTCCGTTTTCTGCCGTCCCCGGGACGATGCCTTCGGCCACTTCACCTGTGGGGAGCGTGGGGAGAAA

GTGTGCAACCCTGGCTGGAAAGGGCCCTACTGCACAGAGCCGATCTGCCTGCCTGGATGTGATGAGCAGC

ATGGATTTTGTGACAAACCAGGGGAATGCAAGTGCAGAGTGGGCTGGCAGGGCCGGTACTGTGACGAGTG

TATCCGCTATCCAGGCTGTCTCCATGGCACCTGCCAGCAGCCCTGGCAGTGCAACTGCCAGGAAGGCTGG

GGGGGCCTTTTCTGCAACCAGGACCTGAACTACTGCACACACCATAAGCCCTGCAAGAATGGAGCCACCT

GCACCAACACGGGCCAGGGGAGCTACACTTGCTCTTGCCGGCCTGGGTACACAGGTGCCACCTGCGAGCT

GGGGATTGACGAGTGTGACCCCAGCCCTTGTAAGAACGGAGGGAGCTGCACGGATCTCGAGAACAGCTAC

TCCTGTACCTGCCCACCCGGCTTCTACGGCAAAATCTGTGAATTGAGTGCCATGACCTGTGCGGACGGCC

CTTGCTTTAACGGGGGTCGGTGCTCAGACAGCCCCGATGGAGGGTACAGCTGCCGCTGCCCCGTGGGCTA

CTCCGGCTTCAACTGTGAGAAGAAAATTGACTACTGCAGCTCTTCACCCTGTTCTAATGGTGCCAAGTGT

GTGGACCTCGGTGATGCCTACCTGTGCCGCTGCCAGGCCGGCTTCTCGGGGAGGCACTGTGACGACAACG

TGGACGACTGCGCCTCCTCCCCGTGCGCCAACGGGGGCACCTGCCGGGATGGCGTGAACGACTTCTCCTG

CACCTGCCCGCCTGGCTACACGGGCAGGAACTGCAGTGCCCCCGTCAGCAGGTGCGAGCACGCACCCTGC

CACAATGGGGCCACCTGCCACGAGAGGGGCCACCGCTATGTGTGCGAGTGTGCCCGAGGCTACGGGGGTC

CCAACTGCCAGTTCCTGCTCCCCGAGCTGCCCCCGGGCCCAGCGGTGGTGGACCTCACTGAGAAGCTAGA

GGGCCAGGGCGGGCCATTCCCCTGGGTGGCCGTGTGCGCCGGGGTCATCCTTGTCCTCATGCTGCTGCTG

GGCTGTGCCGCTGTGGTGGTCTGCGTCCGGCTGAGGCTGCAGAAGCACCGGCCCCCAGCCGACCCCTGCC

GGGGGGAGACGGAGACCATGAACAACCTGGCCAACTGCCAGCGTGAGAAGGACATCTCAGTCAGCATCAT

CGGGGCCACGCAGATCAAGAACACCAACAAGAAGGCGGACTTCCACGGGGACCACAGCGCCGACAAGAAT

GGCTTCAAGGCCCGCTACCCAGCGGTGGACTATAACCTCGTGCAGGACCTCAAGGGTGACGACACCGCCG

TCAGGGACGCGCACAGCAAGCGTGACACCAAGTGCCAGCCCCAGGGCTCCTCAGGGGAGGAGAAGGGGAC

CCCGACCACACTCAGGGGTGGAGAAGCATCTGAAAGAAAAAGGCCGGACTCGGGCTGTTCAACTTCAAAA

GACACCAAGTACCAGTCGGTGTACGTCATATCCGAGGAGAAGGATGAGTGCGTCATAGCAACTGAGGTGT

AA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cell 67:687-699 (1991)

2. Science 268:225-232 (1995)

3. Am. J. Pathol. 154:785-794(1999)

4. Blood 95:1616-1625(2000)

5. Biochem. Biophys. Res. Commun. 276:385-389(2000) 

6.  J. Exp. Med. 194:991-1001(2001)

 

 

Additional supporting documents, including product data sheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.