LINGO1 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1) is also known as LERN1 (Leucine-rich repeat neuronal protein 1) and LRRN6A (Leucine-rich repeat neuronal protein 6A). LINGO1 is a single-pass type I transmembrane protein with a 520 amino acid (aa) extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain. LINGO1 is expressed exclusively in the central nervous system (CNS) with the hghest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression can be detected. LINGO1 is up-regulated in substantia nigra neurons from Parkinson disease patients. LINGO1 is thought to be a functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation.  The receptor complex is responsible for negative regulation of axonal regeneration by myelin-associated factors. LINGO1 may exist as a homotetramer and form a ternary complex with RTN4R/NGFR and RTN4R/TNFRSF19. The intracellular domain of LINGO1 interacts with MYT1L for signal transduction. LINGO1 is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Lingo1 acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.  Inhibition of LINGO1 enhances survival, structure, and function of dopaminergic neurons in Parkinson's disease models.

LINGO1 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1) is also known as LERN1 (Leucine-rich repeat neuronal protein 1) and LRRN6A (Leucine-rich repeat neuronal protein 6A). LINGO1 is a single-pass type I transmembrane protein with a 520 amino acid (aa) extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain. LINGO1 is expressed exclusively in the central nervous system (CNS) with the hghest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression can be detected. LINGO1 is up-regulated in substantia nigra neurons from Parkinson disease patients. LINGO1 is thought to be a functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation.  The receptor complex is responsible for negative regulation of axonal regeneration by myelin-associated factors. LINGO1 may exist as a homotetramer and form a ternary complex with RTN4R/NGFR and RTN4R/TNFRSF19. The intracellular domain of LINGO1 interacts with MYT1L for signal transduction. LINGO1 is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Lingo1 acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.  Inhibition of LINGO1 enhances survival, structure, and function of dopaminergic neurons in Parkinson's disease models.

 

Product Details

 

Gene Symbol: LINGO1; LERN1; LRRN6A; UNQ201; UNQ201/PRO227

 

NCBI Gene ID: 84894

 

Uniprot Entry: Q96FE5

 

Construct Details: The recombinant human LINGO1-Fc is expressed as a 734-amino acid protein consisting of  Cys42 - Thr548 region of LINGO1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

 

Source: Human cells stably expressing human LINGO1-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

CPPRCECSAQ DRAVLCHRKR FVAVPEGIPT ETRLLDLGKN RIKTLNQDEF ASFPHLEELE 

        70         80         90        100        110        120 

LNENIVSAVE PGAFNNLFNL RTLGLRSNRL KLIPLGVFTG LSNLTKLDIS ENKIVILLDY 

       130        140        150        160        170        180 

MFQDLYNLKS LEVGDNDLVY ISHRAFSGLN SLEQLTLEKC NLTSIPTEAL SHLHGLIVLR 

       190        200        210        220        230        240 

LRHLNINAIR DYSFKRLYRL KVLEISHWPY LDTMTPNCLY GLNLTSLSIT HCNLTAVPYL 

       250        260        270        280        290        300 

AVRHLVYLRF LNLSYNPIST IEGSMLHELL RLQEIQLVGG QLAVVEPYAF RGLNYLRVLN 

       310        320        330        340        350        360 

VSGNQLTTLE ESVFHSVGNL ETLILDSNPL ACDCRLLWVF RRRWRLNFNR QQPTCATPEF 

       370        380        390        400        410        420 

VQGKEFKDFP DVLLPNYFTC RRARIRDRKA QQVFVDEGHT VQFVCRADGD PPPAILWLSP 

       430        440        450        460        470        480 

RKHLVSAKSN GRLTVFPDGT LEVRYAQVQD NGTYLCIAAN AGGNDSMPAH LHVRSYSPDW 

       490        500        510        520        530        540 

PHQPNKTFAF ISNQPGEGEA NSTRATVDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI 

       550        560        570        580        590        600 

SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW 

       610        620        630        640        650        660 

LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY 

       670        680        690        700        710        720 

PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH 

       730 

NHYTQKSLSL SPGK 

  

M.W.: Calculated molecular mass (kDa): 83.1; Estimated by SDS-PAGE under reducing condition (kDa): 85-90 

 

Calculated PI: 8.02

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 87875

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. 

 

References

 

1. Eur. J. Neurosci. 18:3167 (2003) 

2. Nat. Neurosci. 7:221-228 (2004) 

3. Nat. Neurosci. 8:745-751 (2005)

4. Neuron 45:345-351 (2005)

5. J. Biol. Chem. 281:36378 (2006) 

6. Proc. Natl. Acad. Sci. 104:14430 (2007)

 

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.