CRTAM (Class-I MHC-restricted T-cell-associated molecule) or CD355 is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Immunoglobulin superfamily (IgSF).  Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Nectin4 contains 1 Ig-like V-type domain and 1 Ig-like C2-type domain in the extracellular region that promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. CRTAM is strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum.  In the immune system, the expression of CRTAM is restricted to activated class-I MHC-restricted cells, including NKT and CD8+ Tcells.  CRTAM interacts with CADM1/Necl2 and promotes natural killer (NK) cell-mediated cytotoxicity and interferon-gamma (IFN-gamma) secretion as well as NK cell-mediated rejection of tumors expressing CADM3/Necl1 in vivo.

CRTAM (Class-I MHC-restricted T-cell-associated molecule) or CD355 is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Immunoglobulin superfamily (IgSF).  Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Nectin4 contains 1 Ig-like V-type domain and 1 Ig-like C2-type domain in the extracellular region that promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. CRTAM is strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum.  In the immune system, the expression of CRTAM is restricted to activated class-I MHC-restricted cells, including NKT and CD8+ Tcells.  CRTAM interacts with CADM1/Necl2 and promotes natural killer (NK) cell-mediated cytotoxicity and interferon-gamma (IFN-gamma) secretion as well as NK cell-mediated rejection of tumors expressing CADM3/Necl1 in vivo.

 

Product Details

 

Gene Symbol: CRTAM; CD355

 

NCBI Gene ID: 56253

 

Uniprot Entry: Q9NQS3

 

Construct Details: Full length human CRTAM gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. 

 

Vector Type: pLTC or pLTC-Marker (lentiviral expression vector containing a heterologous CMV promoter -/+ a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1182 (bp)

 

Gene Insert Sequence:  

ATGTGGTGGAGAGTTCTCAGCTTGCTGGCATGGTTCCCCTTGCAAGAGGCCTCTCTGACTAACCACACAG

AAACCATCACCGTGGAGGAAGGCCAGACGCTCACTCTAAAGTGTGTCACTTCTCTGAGGAAGAACTCCTC

CCTCCAGTGGCTGACCCCCTCAGGGTTCACCATTTTTTTAAATGAGTATCCTGCTTTAAAAAATTCCAAA

TACCAGCTTCTTCATCACTCGGCCAATCAGCTCTCCATCACTGTGCCTAACGTAACCCTGCAAGATGAAG

GCGTGTACAAGTGCTTACATTACAGCGACTCTGTAAGCACAAAGGAAGTGAAAGTGATTGTGCTGGCAAC

TCCTTTCAAGCCAATCCTGGAAGCTTCAGTTATCAGAAAGCAAAATGGAGAAGAACATGTTGTACTCATG

TGCTCCACCATGAGAAGCAAGCCCCCTCCGCAGATAACCTGGCTACTTGGGAATAGCATGGAAGTGTCCG

GTGGAACGCTCCATGAATTTGAAACTGATGGGAAGAAATGTAATACTACCAGCACTCTCATAATCCACAC

TTATGGCAAAAATTCAACGGTGGACTGCATTATCCGACACAGAGGCCTGCAAGGGAGAAAACTAGTAGCA

CCCTTCCGGTTTGAAGATTTGGTTACTGATGAAGAGACAGCTTCAGATGCTCTGGAGAGAAACTCTCTAT

CCTCTCAAGACCCACAGCAGCCCACCAGTACTGTCTCAGTAACGGAAGATTCTAGTACATCGGAGATTGA

CAAGGAAGAGAAAGAACAAACCACTCAAGATCCTGACTTGACCACCGAAGCAAATCCTCAGTATTTAGGA

CTGGCAAGAAAGAAAAGTGGCATCCTGCTGCTCACGCTGGTGTCCTTCCTCATTTTCATACTCTTCATCA

TAGTCCAGCTCTTCATCATGAAGCTGAGGAAAGCACATGTGATATGGAAGAAAGAAAACGAAGTTTCAGA

ACACACACTAGAAAGTTACAGATCAAGGTCAAATAATGAAGAAACATCATCTGAAGAGAAAAATGGCCAA

TCTTCCCACCCTATGCGTTGCATGAACTACATCACAAAGTTGTACTCAGAAGCAAAAACAAAGAGGAAGG

AAAATGTACAACATTCAAAATTAGAAGAAAAGCACATCCAAGTACCAGAGAGTATTGTGTAG

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.