CD124, also known as IL4RA (Interleukin-4 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 4 subfamily.  CD124/IL4RA consists of an extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif and an immunoreceptor tyrosine-based inhibitor motif (ITIM). This ITIM is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation. CD124/IL4RA acts as a receptor for both IL4 and IL13 to regulate IgE production. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are also involved in chemokine and mucus production at sites of allergic inflammation.  A soluble form of CD124 can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in the CD124 gene have been associated with atopy, a condition manifested by allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in the CD124 gene are also associated with resistance to human immunodeficiency virus type-1 infection.

CD124, also known as IL4RA (Interleukin-4 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 4 subfamily.  CD124/IL4RA consists of an extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif and an immunoreceptor tyrosine-based inhibitor motif (ITIM). This ITIM is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation. CD124/IL4RA acts as a receptor for both IL4 and IL13 to regulate IgE production. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are also involved in chemokine and mucus production at sites of allergic inflammation.  A soluble form of CD124 can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in the CD124 gene have been associated with atopy, a condition manifested by allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in the CD124 gene are also associated with resistance to human immunodeficiency virus type-1 infection.

 

Product Details

 

Gene Symbol: IL4R; IL4RA; CD124; IL4RA; IL-4RA; IL4BP

 

NCBI Gene ID: 3566

 

Uniprot Entry: P24394

 

Construct Details: The recombinant human CD124-Fc protein is expressed as a 430 amino acid protein consisting of  Met26 - Glu227 region of CD124 fused to the Fc from human IgG1, which exists as a dimer under non-reducing conditions.  CD124 ECD contains 6 potential N-linked glycosylation sites.

 

Source: Human cells stably expressing CD124-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

MKVLQEPTCV SDYMSISTCE WKMNGPTNCS TELRLLYQLV FLLSEAHTCI PENNGGAGCV 

        70         80         90        100        110        120 

CHLLMDDVVS ADNYTLDLWA GQQLLWKGSF KPSEHVKPRA PGNLTVHTNV SDTLLLTWSN 

       130        140        150        160        170        180 

PYPPDNYLYN HLTYAVNIWS ENDPADFRIY NVTYLEPSLR IAASTLKSGI SYRARVRAWA 

       190        200        210        220        230        240 

QCYNTTWSEW SPSTKWHNSY RESTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP 

       250        260        270        280        290        300 

EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK 

       310        320        330        340        350        360 

EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI 

       370        380        390        400        410        420 

AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT 

       430 

QKSLSLSPGK 

 

 

  

M.W.: Calculated molecular mass (kDa): 48.7; Estimated by SDS-PAGE under reducing condition (kDa): ~65

 

Calculated PI: 6.36

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  103540

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binding to human IL4 and IL13.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.