CD126, also known as IL6RA (Interleukin-6 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 3 subfamily.  IL6RA/CD126 consists of an extracellular region with 1 Ig-like C2-type (immunoglobulin-like) and 2 fibronectin type III (FN3) domains, in addition to a transmembrane (TM) domain, and a cytoplasmic tail. IL6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. IL6RA/CD126 itself binds to IL6 with low affinity and does not transduce a signal. IL6RA/CD126 is a subunit of the IL6 receptor complex (IL6R) consisting of CD126 and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Signal activation requires IL6ST and activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Dysregulated production of IL6 and its receptor IL6RA are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Low concentration of a soluble form of IL6RA may act as an agonist of IL6 activity.

CD126, also known as IL6RA (Interleukin-6 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 3 subfamily.  IL6RA/CD126 consists of an extracellular region with 1 Ig-like C2-type (immunoglobulin-like) and 2 fibronectin type III (FN3) domains, in addition to a transmembrane (TM) domain, and a cytoplasmic tail. IL6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. IL6RA/CD126 itself binds to IL6 with low affinity and does not transduce a signal. IL6RA/CD126 is a subunit of the IL6 receptor complex (IL6R) consisting of CD126 and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Signal activation requires IL6ST and activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Dysregulated production of IL6 and its receptor IL6RA are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Low concentration of a soluble form of IL6RA may act as an agonist of IL6 activity.

 

Product Details

 

Gene Symbol: IL6R; CD126; IL6Q; gp80; IL6RA; IL6RQ; IL-6RA; IL-6R-1; gp80

 

NCBI Gene ID: 3570

 

Uniprot Entry: P08887

 

Construct Details: The recombinant human CD126-Fc protein is expressed as a 570 amino acid protein consisting of  Leu20 - Val362 region of CD126 fused to the Fc from human IgG1, which exists as a dimer under non-reducing conditions. CD126 ECD contains 5 potential N-linked glycosylation sites.

 

Source: Human cells stably expressing CD126-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKPAAGS HPSRWAGMGR 

        70         80         90        100        110        120 

RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VVCEWGPRST 

       130        140        150        160        170        180 

PSLTTKAVLL VRKFQNSPAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG 

       190        200        210        220        230        240 

SKFSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSSFY RLRFELRYRA 

       250        260        270        280        290        300 

ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQLRAQEEFG QGEWSEWSPE AMGTPWTESR 

       310        320        330        340        350        360 

SPPAENEVST PMQALTTNKD DDNILFRDSA NATSLPVQDS SSVDKTHTCP PCPAPELLGG 

       370        380        390        400        410        420 

PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN 

       430        440        450        460        470        480 

STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE 

       490        500        510        520        530        540 

MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW 

       550        560        570 

QQGNVFSCSV MHEALHNHYT QKSLSLSPGK 

 

 

  

M.W.: Calculated molecular mass (kDa): 63.8; Estimated by SDS-PAGE under reducing condition (kDa): ~80-85

 

Calculated PI: 7.02

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  105850

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binding to human IL6.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.