Human ILDR2/Angulin-3 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

ILDR2 (immunoglobulin (Ig)-like domain containing receptor 2) or angulin-3 belongs to the LISCH7 family of the Ig superfamily (IgSF).  ILDR2 is a single-pass type I transmembrane proteins with an extracellular domain containing an Ig-like V-type motif, a transmembrane domain (TM), and a cytoplasmic tail enriched with basic/acidic/polar residues.  Similar to other family members ILDR1 and LSR, ILDR2 may be involved in the maintain of the epithelial barrier function through the recruitment of MARVELD2/tricellulin to tricellular tight junctions (tTJs).  ILDR2 may be involved in ER stress pathways with effects on lipid homeostasis and insulin secretion.  ILDR2 may also be Involved in negative regulation of T cell activation and function as a B7-like protein family member  on immune cells and inflamed tissues with T-cell inhibitory activity.

 

 

Gene Symbol: ILDR2; Angulin-2; C1orf32

 

NCBI Gene ID: 387597

 

Uniprot Entry: Q71H61

 

Construct Details: Full length human ILDR2 gene (encoding UniProt #Q71H61-1) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1920 (bp)

 

Gene Insert Sequence:  

ATGGATAGGGTCTTGCTGAGGTGGATTTCTCTCTTCTGGCTAACAGCCATGGTCGAAGGCCTTCAGGTCA

CAGTGCCCGACAAGAAGAAGGTGGCCATGCTCTTCCAGCCCACTGTGCTTCGCTGCCACTTCTCAACATC

CTCCCATCAGCCTGCAGTTGTGCAGTGGAAGTTCAAGTCCTACTGCCAGGATCGCATGGGAGAATCCTTG

GGCATGTCCTCTACCCGGGCCCAATCTCTCAGCAAGAGAAACCTGGAATGGGACCCCTACTTGGATTGTT

TGGACAGCAGGAGGACTGTTCGAGTAGTAGCTTCAAAACAGGGCTCGACTGTCACCCTGGGAGATTTCTA 

CAGGGGCAGAGAGATCACGATTGTTCATGATGCAGATCTTCAAATTGGAAAGCTTATGTGGGGAGACAGC 

GGACTCTATTACTGTATTATCACCACCCCAGATGACCTGGAGGGGAAAAATGAGGACTCAGTGGAACTGC 

TGGTGTTGGGCAGGACAGGGCTGCTTGCTGATCTCTTGCCCAGTTTTGCTGTGGAGATTATGCCAGAGTG 

GGTGTTTGTTGGCCTGGTGCTCCTGGGCGTCTTCCTCTTCTTCGTCCTGGTGGGGATCTGCTGGTGCCAG 

TGCTGCCCTCACAGCTGCTGCTGCTATGTCCGCTGCCCATGCTGCCCAGATTCCTGCTGCTGCCCTCAAG

CCTTGTATGAAGCAGGGAAAGCAGCAAAGGCCGGGTACCCTCCCTCTGTCTCCGGTGTCCCCGGCCCTTA 

CTCCATCCCCTCTGTCCCTTTGGGAGGAGCCCCCTCATCTGGCATGCTGATGGACAAGCCGCATCCACCT 

CCCTTGGCACCAAGTGACTCCACTGGAGGAAGCCACAGTGTTCGCAAAGGTTACCGGATCCAGGCTGACA 

AAGAGAGAGACTCCATGAAGGTCCTGTACTATGTTGAGAAGGAGCTGGCTCAGTTTGATCCAGCCAGAAG 

GATGAGAGGCAGATATAACAACACCATCTCAGAACTCAGCTCCCTACATGAGGAGGACAGCAATTTCCGC 

CAGTCTTTCCATCAGATGAGAAGCAAGCAGTTCCCTGTGTCTGGGGACTTGGAGAGCAATCCTGACTATT 

GGTCAGGTGTCATGGGAGGCAGCAGTGGGGCAAGCCGCGGGCCCTCAGCCATGGAGTATAACAAAGAGGA 

TCGAGAGAGCTTCAGGCACAGCCAGCCGCGCTCCAAGTCGGAGATGCTGTCGCGGAAGAACTTCGCCACG 

GGGGTGCCGGCCGTTTCCATGGACGAGCTGGCGGCCTTCGCTGACTCCTACGGCCAGCGGCCCCGCCGGG 

CAGACGGCAACAGTCACGAGGCGCGGGGCGGGAGCCGCTTCGAGCGCTCGGAGTCGCGGGCGCACAGCGG 

CTTCTACCAGGACGACTCCTTGGAGGAGTACTACGGTCAGCGCAGCCGCAGCCGCGAGCCCCTGACCGAT 

GCTGACCGCGGCTGGGCCTTCAGCCCCGCGCGCCGCAGACCCGCCGAGGACGCGCACCTGCCGCGGCTGG 

TGAGCCGCACGCCAGGCACCGCACCCAAATACGACCACTCGTACCTGGGCAGCGCGCGGGAGCGCCAGGC 

GCGGCCCGAGGGCGCCAGCCGCGGTGGCAGCCTGGAGACGCCATCCAAGCGGAGCGCGCAGCTCGGCCCG 

CGCAGCGCCTCCTACTACGCTTGGTCGCCGCCCGGCACCTACAAGGCCGGCTCGTCGCAGGACGACCAGG 

AGGACGCGTCCGACGACGCGCTGCCGCCCTACAGCGAGCTGGAGCTGACCCGCGGCCCGTCCTACCGCGG 

CCGCGACCTGCCCTACCACAGCAACTCGGAGAAGAAGAGGAAAAAGGAGCCCGCCAAGAAAACCAATGAC 

TTTCCAACCAGGATGTCCCTTGTGGTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.