UMOD (Uromodulin) or THP (Tamm-Horsfall urinary glycoprotein) is the most abundant protein in urine under physiological conditions.  UMOD is a glycosyl phosphatidylinositol (GPI)-anchored protein that is situated on the luminal cell surface of the loop of Henle.  UMOD can be secreted into urine following proteolytic cleavage of its extracellular region that contains 4 EGF-like motifs and a ZP domain that mediates polymerization, leading to the formation of long filaments.  UMOD functions in biogenesis of apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure to mediate the water barrier permeability. UMOD may also serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF.  In the urine, UMOD may contribute to colloid osmotic pressure, retard passage of positively charged electrolytes, and act as a constitutive inhibitor of calcium crystallization.  Its excretion into urine provides defense against urinary tract infections.  Defects in the UMOD gene are associated with several renal disorders, including tubulointerstitial kidney disease, autosomal dominant, 1 (ADTKD1), medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN).

UMOD (Uromodulin) or THP (Tamm-Horsfall urinary glycoprotein) is the most abundant protein in urine under physiological conditions.  UMOD is a glycosyl phosphatidylinositol (GPI)-anchored protein that is situated on the luminal cell surface of the loop of Henle.  UMOD can be secreted into urine following proteolytic cleavage of its extracellular region that contains 4 EGF-like motifs and a ZP domain that mediates polymerization, leading to the formation of long filaments.  UMOD functions in biogenesis of apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure to mediate the water barrier permeability. UMOD may also serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF.  In the urine, UMOD may contribute to colloid osmotic pressure, retard passage of positively charged electrolytes, and act as a constitutive inhibitor of calcium crystallization.  Its excretion into urine provides defense against urinary tract infections.  Defects in the UMOD gene are associated with several renal disorders, including tubulointerstitial kidney disease, autosomal dominant, 1 (ADTKD1), medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN).

 

Product Details

 

Gene Symbol: UMOD; THP; Uromodulin; FJHN; HNFJ; THGP; HNFJ1; MCKD2; ADTKD1; ADMCKD2

 

NCBI Gene ID: 7369

 

Uniprot Entry: P07911

 

Construct Details: Full length human UMOD gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1923 (bp)

 

Gene Insert Sequence:  

ATGGGGCAGCCATCTCTGACTTGGATGCTGATGGTGGTGGTGGCCTCTTGGTTCATCACAACTGCAGCCA

CTGACACCTCAGAAGCAAGATGGTGCTCTGAATGTCACAGCAATGCCACCTGCACGGAGGATGAGGCCGT

TACGACGTGCACCTGTCAGGAGGGCTTCACCGGCGATGGCCTGACCTGCGTGGACCTGGATGAGTGCGCC

ATTCCTGGAGCTCACAACTGCTCCGCCAACAGCAGCTGCGTAAACACGCCAGGCTCCTTCTCCTGCGTCT

GCCCCGAAGGCTTCCGCCTGTCGCCCGGTCTCGGCTGCACAGACGTGGATGAGTGCGCTGAGCCTGGGCT

TAGCCACTGCCACGCCCTGGCCACATGTGTCAATGTGGTGGGCAGCTACTTGTGCGTATGCCCCGCGGGC

TACCGGGGGGATGGATGGCACTGTGAGTGCTCCCCGGGCTCCTGCGGGCCGGGGTTGGACTGCGTGCCCG

AGGGCGACGCGCTCGTGTGCGCGGATCCGTGCCAGGCGCACCGCACCCTGGACGAGTACTGGCGCAGCAC

CGAGTACGGGGAGGGCTACGCCTGCGACACGGACCTGCGCGGCTGGTACCGCTTCGTGGGCCAGGGCGGT

GCGCGCATGGCCGAGACCTGCGTGCCAGTCCTGCGCTGCAACACGGCCGCCCCCATGTGGCTCAATGGCA

CGCATCCGTCCAGCGACGAGGGCATCGTGAGCCGCAAGGCCTGCGCGCACTGGAGCGGCCACTGCTGCCT

GTGGGATGCGTCCGTCCAGGTGAAGGCCTGTGCCGGCGGCTACTACGTCTACAACCTGACAGCGCCCCCC

GAGTGTCACCTGGCGTACTGCACAGACCCCAGCTCCGTGGAGGGGACGTGTGAGGAGTGCAGTATAGACG

AGGACTGCAAATCGAATAATGGCAGATGGCACTGCCAGTGCAAACAGGACTTCAACATCACTGATATCTC

CCTCCTGGAGCACAGGCTGGAATGTGGGGCCAATGACATGAAGGTGTCGCTGGGCAAGTGCCAGCTGAAG

AGTCTGGGCTTCGACAAGGTCTTCATGTACCTGAGTGACAGCCGGTGCTCGGGCTTCAATGACAGAGACA

ACCGGGACTGGGTGTCTGTAGTGACCCCAGCCCGGGATGGCCCCTGTGGGACAGTGTTGACGAGGAATGA

AACCCATGCCACTTACAGCAACACCCTCTACCTGGCAGATGAGATCATCATCCGTGACCTCAACATCAAA

ATCAACTTTGCATGCTCCTACCCCCTGGACATGAAAGTCAGCCTGAAGACCGCCCTACAGCCAATGGTCA

GTGCTCTAAACATCAGAGTGGGCGGGACCGGCATGTTCACCGTGCGGATGGCGCTCTTCCAGACCCCTTC

CTACACGCAGCCCTACCAAGGCTCCTCCGTGACACTGTCCACTGAGGCTTTTCTCTACGTGGGCACCATG

TTGGATGGGGGCGACCTGTCCCGATTTGCACTGCTCATGACCAACTGCTATGCCACACCCAGTAGCAATG

CCACGGACCCCCTGAAGTACTTCATCATCCAGGACAGATGCCCACACACTAGAGACTCAACTATCCAAGT

GGTGGAGAATGGGGAGTCCTCCCAGGGCCGATTTTCCGTCCAGATGTTCCGGTTTGCTGGAAACTATGAC

CTAGTCTACCTGCACTGTGAAGTCTATCTCTGTGACACCATGAATGAAAAGTGCAAGCCTACCTGCTCTG

GGACCAGATTCCGAAGTGGGAGTGTCATAGATCAATCCCGTGTCCTGAACTTGGGTCCCATCACACGGAA

AGGTGTCCAGGCCACAGTCTCAAGGGCTTTTAGCAGCTTGGGGCTCCTGAAAGTCTGGCTGCCTCTGCTT

CTCTCGGCCACCTTGACCCTGACTTTTCAGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

  

 

Product datasheet (pdf) and additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.