Angiotensin-converting enzymes including ACE and ACE2 are a central component of the renin-angiotensin system (RAS) that controls blood pressure by regulating the volume of fluids in the body. Both ACE & ACE2 are single-pass type I membrane proteins that belong to the peptidase M2 family.  Unlike ACE, the extracellular region of ACE2 contains only 1 zinc-binding metallopeptidase domain (19-611aa) and a C-terminal domain (612-740aa) that is 48% identical to human collectrin.  Its cytoplasmic tail contains several motifs such as LIR, PDZ-binding, PTB and endocytic sorting signals allowing interactions with proteins that mediate endocytic trafficking and autophagy.  The secreted ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is expressed in various human organs and may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, ACE2 is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). 

Angiotensin-converting enzymes including ACE and ACE2 are a central component of the renin-angiotensin system (RAS) that controls blood pressure by regulating the volume of fluids in the body. Both ACE & ACE2 are single-pass type I membrane proteins that belong to the peptidase M2 family.  Unlike ACE, the extracellular region of ACE2 contains only 1 zinc-binding metallopeptidase domain (19-611aa) and a C-terminal domain (612-740aa) that is 48% identical to human collectrin.  Its cytoplasmic tail contains several motifs such as LIR, PDZ-binding, PTB and endocytic sorting signals allowing interactions with proteins that mediate endocytic trafficking and autophagy.  The secreted ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is expressed in various human organs and may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, ACE2 is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). 

 

Product Details

 

Gene Symbol: ACE2; ACEH

 

NCBI Gene ID: 59272

 

Uniprot Entry: Q9BYF1

 

Construct Details: The recombinant human ACE2-Fc fusion is expressed as a 823 amino acid protein consisting of Gln18 - Pro612 region (metallopeptidase/catalytic domain only) of ACE2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.  

  

Source: Human cells stably expressing ACE2c-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS 

        70         80         90        100        110        120 

TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN 

       130        140        150        160        170        180 

PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE 

       190        200        210        220        230        240 

DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS 

       250        260        270        280        290        300 

PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS  

       310        320        330        340        350        360 

VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG  

       370        380        390        400        410        420 

HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN  

       430        440        450        460        470        480 

FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY  

       490        500        510        520        530        540 

CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM  

       550        560        570        580        590        600 

LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPSTGTH  

       610        620        630        640        650        660 

TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV  

       670        680        690        700        710        720 

HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR  

       730        740        750        760        770        780 

EPQVYTLPPS REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF  

       790        800        810        820 

FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK

 

 

  

M.W.: Calculated molecular mass (kDa): 94.4; Estimated by SDS-PAGE under reducing condition (kDa): ~100

 

Calculated PI: 5.37

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  186515

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.