Monkey B7-H4 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
B7H4 (B7 homolog 4), also known as B7X, B7H4, B7S1, and VTCN1, is a single pass type I transmembrane glycoprotein that belongs to the B7 family of the Immunoglobulin (Ig) superfamily. Like other B7 family members, B7-H4 contains one Ig V-like and one Ig C-like domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7H4 is expressed on the surface of activated lymphocytes, macrophages, monocytes, dendritic cells, epithelial cells, and bone marrowderived mesenchymal stem cells. It can be up-regulated by IL6 and IL10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7-H4 plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7H4 is also involved in promoting epithelial cell transformation. B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Gene Symbol: B7-H4; VTCN1; B7H4; B7x; B7S1; B7h.5; PRO1291; UNQ659/PRO1291
NCBI Gene ID: 714123
Uniprot Entry: F7B770
Construct Details: The recombinant monkey B7-H4-Fc fusion protein is expressed as a 463 amino acid protein consisting of Leu25 - Ser259 region of B7-H4 (UniProt accession #Q7Z7D3) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source: Human cells stably expressing monkey B7-H4-Fc and growing in chemical-defined media with no animal components or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
LIIGFGISGR HSITVTTVAS AGNIGEDGIL SCTFEPDIKL SDIVIQWLKE GVIGLVHEFK
70 80 90 100 110 120
EGKDELSEQD EMFRGRTAVF ADQVIVGNAS LRLKNVQLTD AGTYKCYIIT SKGKGNANLE
130 140 150 160 170 180
YKTGAFSMPE VNVDYNASSE TLRCEAPRWF PQPTVVWASQ VDQGANFSEV SNTSFELNSE
190 200 210 220 230 240
NVTMKVVSVL YNVTINNTYS CMIENDIAKA TGDIKVTESE IKRRSHLQLL NSKASSTGTH
250 260 270 280 290 300
TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV
310 320 330 340 350 360
HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR
370 380 390 400 410 420
EPQVYTLPPS REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF
430 440 450 460
FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK
M.W.: Calculated molecular mass 51.4 kDa; estimated by SDS-PAGE under reducing condition ~75 kDa probably due to glycosylation
Calculated PI: 5.89
Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 61475
Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
1. Proc. Natl. Acad. Sci. 100:10388 (2003).
2. Immunity 18:863 (2003).
3. Immunity 18:849 (2003).
4. Clin. Cancer Res. 11:1842 (2005).
5. J. Exp. Med. 203:871 (2006).
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
Human B7-H4 Full-length Gene in Lentivector, Endotoxin-free DNA
Human B7-H4 Protein, ECD, Fc-fusion, Biotinylated, Recombinant