EPOR (erythropoietin receptor) is a single pass type I transmembrane glycoprotein of the type I cytokine receptor family (type 1 subfamily). EPOR is the erythropoietin receptor. Upon ligand binding, EPOR activates JAK2 (Janus kinase) tyrosine kinase which in turn activates different intracellular pathways including: Ras/MAP kinase (MAPK), phosphatidylinositol 3-kinase (PI3K) and STAT transcription factors.  EPOR consists of a 226 amino acid (aa) extracellular region with 1 fibronectin type III (FN3) domain and a juxtamembrane WSXWS motif, a transmembrane domain, and a 235 aa cytoplasmic region with a Box 1 motif and an ITIM (immunoreceptor tyrosine-based inhibitor motif). The WSXWS motif may be necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding.  The box 1 motif is required for JAK interaction/activation while the ITIM is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.that mediates the activation of JAK- Stat and MAPK as well as PI3K pathways.  EPOR plays an important role in erythroid cell proliferation and differentiation. Defects in the EPOR gene may produce erythroleukemia and familial erythrocytosis. Dysregulation of the EPOR gene may affect the growth of certain tumors.

EPOR (erythropoietin receptor) is a single pass type I transmembrane glycoprotein of the type I cytokine receptor family (type 1 subfamily). EPOR is the erythropoietin receptor. Upon ligand binding, EPOR activates JAK2 (Janus kinase) tyrosine kinase which in turn activates different intracellular pathways including: Ras/MAP kinase (MAPK), phosphatidylinositol 3-kinase (PI3K) and STAT transcription factors.  EPOR consists of a 226 amino acid (aa) extracellular region with 1 fibronectin type III (FN3) domain and a juxtamembrane WSXWS motif, a transmembrane domain, and a 235 aa cytoplasmic region with a Box 1 motif and an ITIM (immunoreceptor tyrosine-based inhibitor motif). The WSXWS motif may be necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding.  The box 1 motif is required for JAK interaction/activation while the ITIM is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.that mediates the activation of JAK- Stat and MAPK as well as PI3K pathways.  EPOR plays an important role in erythroid cell proliferation and differentiation. Defects in the EPOR gene may produce erythroleukemia and familial erythrocytosis. Dysregulation of the EPOR gene may affect the growth of certain tumors.

 

Product Details

 

Gene Symbol: EPOR; EPO-R

 

NCBI Gene ID: 2057

 

Uniprot Entry: P19235

 

Construct Details: The recombinant human EPOR-Fc is expressed as a 452 amino acid protein consisting of Ala25 - Leu248 region of EPOR (UniProt accession #P19235) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.  

 

Source: Human cells stably expressing EPOR-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE  

        70         80         90        100        110        120 

DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL  

       130        140        150        160        170        180 

LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE  

       190        200        210        220        230        240 

CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLSTGTHT CPPCPAPELL  

       250        260        270        280        290        300 

GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ 

       310        320        330        340        350        360 

YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR 

       370        380        390        400        410        420 

EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS 

       430        440        450 

RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK 

 

 

  

M.W.: Calculated molecular mass (kDa): 50.2; Estimated by SDS-PAGE under reducing condition (kDa): ~55

 

Calculated PI: 6.11

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  76485

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.