Human PRLR/HPRL/MFAB Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant

Description

PRLR (prolactin receptor) is a single pass type I transmembrane glycoprotein of the type I cytokine receptor family (type 1 subfamily). PRLR consists of a 210 amino acid (aa) extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a 364 aa cytoplasmic region with a Box 1 motif.  The WSXWS motif may be necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding.  The box 1 motif is required for JAK interaction/activation.  PRLR is a receptor for the anterior pituitary hormone prolactin (PRL). Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of PRLR. PRLR acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT.  Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms of PRLR exist and may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer.

 

 

Gene Symbol: PRLR; HPRL; MFAB; hPRLrI

 

NCBI Gene ID: 5618

 

Uniprot Entry: P16471

 

Construct Details: The recombinant human PRLR-Fc is expressed as a 438-amino acid protein consisting of Gln25 -  Glu234 region of PRLR and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

   

Source: Human cells stably expressing PRLR-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QLPPGKPEIF KCRSPNKETF TCWWRPGTDG GLPTNYSLTY HREGETLMHE CPDYITGGPN 

        70         80         90        100        110        120 

SCHFGKQYTS MWRTYIMMVN ATNQMGSSFS DELYVDVTYI VQPDPPLELA VEVKQPEDRK 

       130        140        150        160        170        180 

PYLWIKWSPP TLIDLKTGWF TLLYEIRLKP EKAAEWEIHF AGQQTEFKIL SLHPGQKYLV  

       190        200        210        220        230        240 

QVRCKPDHGY WSAWSPATFI QIPSDFTMND SSGTHTCPPC PAPELLGGPS VFLFPPKPKD 

       250        260        270        280        290        300 

TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL 

       310        320        330        340        350        360 

HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV 

       370        380        390        400        410        420 

KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH 

       430 

EALHNHYTQK SLSLSPGK

 

   

M.W.: Calculated molecular mass (kDa): 50.0; Estimated by SDS-PAGE under reducing condition (kDa): ~55-60

 

Calculated PI: 6.38

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  101925

  

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.