Human SCCA1/SERPINB3/T4-A Protein, Recombinant

SCCA1 (squamous cell carcinoma antigen 1), also known as SERPINB3 (serpin family B member 3) and Protein T4-A, is a cysteine-type endopeptidase inhibitor of the Ov-serpin subfamily of the serpin family. SCCA1 has protease binding and virus receptor activities. SCCA1 is located in several subcellular components, including cytoplasmic vesicle; cytosol; and extracellular exosome. SCCA1 is involved in several biological processes, including autocrine signaling; paracrine signaling, and regulation of protein metabolic process. SCCA1 is restrictedly expressed in esophagus and its expression is closely related to cellular differentiation in both normal and malignant squamous cells.  SCCA1 acts as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. It also functions as an inhibitor of UV-induced apoptosis. SCCA1 is strongly up-regulated in the upper epidermis of sun-exposed skin and can be secreted in plasma by cancerous cells. 

Gene Symbol: SCCA1; SERPINB3; SCC; T4-A; SSCA1; SCCA-1; HsT1196; SCCA-PD

NCBI Gene ID: 6317

Uniprot Entry: P29508

Construct Details: The recombinant human SCCA1 is expressed as a 400 amino acid protein consisting of Asn2 - Pro390 region of and a and a C-terminal His-tag.

Source: Human cells stably expressing SCCA1 and growing in chemical-defined media with no animal component or antibiotics

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
NSLSEANTKF MFDLFQQFRK SKENNIFYSP ISITSALGMV LLGAKDNTAQ QIKKVLHFDQ

        70         80         90        100        110        120
VTENTTGKAA TYHVDRSGNV HHQFQKLLTE FNKSTDAYEL KIANKLFGEK TYLFLQEYLD

       130        140        150        160        170        180
AIKKFYQTSV ESVDFANAPE ESRKKINSWV ESQTNEKIKN LIPEGNIGSN TTLVLVNAIY

       190        200        210        220        230        240
FKGQWEKKFN KEDTKEEKFW PNKNTYKSIQ MMRQYTSFHF ASLEDVQAKV LEIPYKGKDL

       250        260        270        280        290        300
SMIVLLPNEI DGLQKLEEKL TAEKLMEWTS LQNMRETRVD LHLPRFKVEE SYDLKDTLRT

       310        320        330        340        350        360
MGMVDIFNGD ADLSGMTGSR GLVLSGVLHK AFVEVTEEGA EAAAATAVVG FGSSPTSTNE

       370        380        390        400
EFHCNHPFLF FIRQNKTNSI LFYGRFSSPS TGHHHHHHHH

M.W.: Calculated molecular mass 45.8 kDa; estimated by SDS-PAGE under reducing condition: ~50-55 kDa 

Calculated PI: 6.66

Calculated extinction coefficients (M-1 cm-1, at 280nm):  39880

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.