ASTN1 (Astrotactin-1) is a two-pass type I transmembrane protein that belongs to the astrotactin family.  ASTN1 contains 2 extracellular domains (ECD), including a 132 aa (amino acid) long small ECD1 and a 900 aa long large ECD2.  The ECD2 is composed of three EGF-like motifs and one Fibronectin type-III motif.  ASTN1 is known to interact with ASTN2 in a calcium-independent manner.  ASTN1 functions as a neuronal adhesion molecule and is required for glial-guided migration of young postmitotic neuroblasts in cortical regions of developing brain, including cerebrum, hippocampus, cerebellum, and olfactory bulb. ASTN1 is also required for normal rate of migration of granule cells during brain development and for normal cerebellum development.

ASTN1 (Astrotactin-1) is a two-pass type I transmembrane protein that belongs to the astrotactin family.  ASTN1 contains 2 extracellular domains (ECD), including a 132 aa (amino acid) long small ECD1 and a 900 aa long large ECD2.  The ECD2 is composed of three EGF-like motifs and one Fibronectin type-III motif.  ASTN1 is known to interact with ASTN2 in a calcium-independent manner.  ASTN1 functions as a neuronal adhesion molecule and is required for glial-guided migration of young postmitotic neuroblasts in cortical regions of developing brain, including cerebrum, hippocampus, cerebellum, and olfactory bulb. ASTN1 is also required for normal rate of migration of granule cells during brain development and for normal cerebellum development.

 

Product Details

 

Gene Symbol: ASTN1; Astrotactin-1; ASTN

 

NCBI Gene ID: 460

 

Uniprot Entry: O14525

 

Construct Details: Full length human ASTN1 gene (encoding Uniprot# O14525-2) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3885 (bp)

 

Gene Insert Sequence:  

ATGGCTTTAGCCGGGCTCTGCGCCCTGCTCGCCTGCTGCTGGGGGCCGGCGGCGGTGCTGGCCACGGCCG
CCGGCGACGTGGATCCATCCAAGGAGCTGGAGTGCAAGCTCAAAAGCATCACGGTGTCGGCACTGCCCTT
CCTGCGCGAGAACGACCTGAGCATCATGCACAGCCCCTCGGCCTCGGAGCCCAAGCTCCTCTTCTCGGTG
CGCAACGACTTCCCGGGAGAAATGGTCGTGGTGGACGACCTGGAGAACACGGAGCTGCCCTACTTCGTGC
TGGAGATCTCAGGGAACACAGAGGATATCCCTTTGGTGCGCTGGAGGCAGCAGTGGCTGGAGAATGGCAC
TTTGCTTTTTCACATTCATCACCAAGATGGTGCCCCAAGCCTTCCTGGACAAGACCCCACTGAAGAACCC
CAACATGAGTCGGCAGAAGAGGAGCTGAGGATCCTCCACATCTCAGTCATGGGTGGCATGATCGCTCTGC
TGCTGTCCATCTTGTGCCTGGTGATGATCCTGTATACTCGCCGGCGCTGGTGCAAACGCCGCCGGGTCCC
GCAGCCCCAGAAGAGTGCCAGTGCTGAGGCAGCCAATGAGATTCACTACATTCCTTCTGTGCTGATCGGC
GGGCACGGACGGGAGAGCCTGCGCAATGCCCGCGTGCAGGGCCACAACTCCAGTGGCACCCTGAGCATCC
GGGAGACACCTATCCTGGACGGCTATGAGTATGACATCACTGATCTGCGCCACCATCTGCAGAGGGAGTG
CATGAACGGAGGGGAGGACTTTGCCAGCCAGGTCACGCGCACCCTCGACTCCCTGCAGGGCTGCAATGAA
AAGTCGGGGATGGACCTCACACCAGGAAGTGACAATGCCAAGCTGTCACTGATGAACAAGTATAAAGATA
ATATTATAGCCACTAGCCCTGTGGACTCCAACCACCAGCAAGCCACCCTTCTCTCTCACACCTCCAGCAG
CCAGAGAAAGCGGATCAACAACAAAGCAAGAGCTGGTTCCGCCTTCTTGAACCCTGAAGGGGATTCTGGC
ACAGAGGCAGAAAACGACCCCCAGCTGACCTTTTACACGGATCCTTCAAGGAGCAGGAGGCGTAGTAGAG
TGGGTTCTCCCCGAAGTCCTGTGAATAAGACCACCTTGACCCTGATCAGCATCACCAGCTGTGTGATTGG
CCTCGTGTGCTCCTCTCACGTCAACTGCCCTCTCGTTGTCAAGATCACCCTGCATGTCCCTGAGCACCTG
ATTGCTGATGGGAGCCGCTTCATCTTGCTGGAGGGGAGCCAGCTGGATGCCAGTGACTGGCTGAACCCTG
CCCAAGTGGTTCTCTTCTCTCAGCAGAACTCCAGCGGACCCTGGGCCATGGACCTCTGTGCCCGGCGGCT
CCTGGACCCCTGTGAACACCAATGTGACCCCGAAACTGGGGAATGCCTCTGCTATGAAGGCTACATGAAG
GATCCAGTACATAAGCACCTTTGCATTCGGAACGAATGGGGGACAAACCAGGGGCCATGGCCTTACACAA
TATTTCAGCGAGGCTTTGACCTGGTTTTGGGAGAGCAGCCCTCTGATAAAATATTTAGATTCACCTACAC
TCTTGGGGAGGGCATGTGGTTGCCCCTCAGCAAGAGCTTTGTGATTCCACCAGCCGAACTGGCCATCAAT
CCATCAGCAAAGTGCAAGACGGACATGACTGTGATGGAGGATGCTGTGGAGGTCAGAGAGGAGCTGATGA
CTTCATCCTCCTTCGACAGCCTGGAGGTTCTCTTAGATTCCTTTGGGCCGGTGCGCGACTGCAGCAAAGA
TAACGGGGGCTGCAGTAAGAATTTCCGCTGTATTTCAGATCGCAAGCTGGACTCCACTGGTTGCGTGTGC
CCATCTGGACTCAGTCCCATGAAGGACAGCTCTGGCTGCTATGACCGCCACATCGGGGTGGACTGTTCCG
ACGGCTTCAACGGCGGCTGTGAGCAGCTGTGCCTCCAGCAGATGGCGCCCTTCCCGGACGACCCCACCTT
GTATAACATCCTCATGTTCTGTGGGTGCATCGAGGACTACAAGCTTGGTGTGGATGGACGCTCTTGCCAA
CTCATCACGGAGACCTGTCCAGAGGGAAGTGACTGTGGGGAAAGCAGGGAGCTTCCCATGAACCAGACCC
TCTTTGGGGAGATGTTCTTTGGTTACAACAACCATTCCAAGGAAGTGGCTGCCGGACAGGTGCTGAAAGG
AACATTCAGGCAAAACAACTTTGCTCGTGGTTTAGACCAGCAACTGCCAGATGGTCTTGTGGTGGCCACT
GTGCCCCTGGAGAATCAATGCCTAGAGGAGATCTCGGAGCCCACCCCTGACCCTGACTTCCTGACTGGGA
TGGTGAACTTCAGTGAAGTGTCTGGGTACCCTGTGCTGCAGCACTGGAAGGTCCGGTCTGTGATGTACCA
CATCAAACTCAACCAAGTGGCCATCTCTCAGGCCCTCAGCAATGCTCTCCACTCGCTGGATGGGGCTACA
TCTCGTGCAGATTTTGTGGCGCTGTTGGACCAGTTCGGCAACCATTACATCCAGGAAGCTATCTACGGCT
TTGAGGAGTCCTGTTCTATCTGGTACCCAAACAAGCAGGTCCAGCGGCGACTCTGGCTGGAGTATGAAGA
CATCAGTAAAGGCAACTCCCCATCAGATGAGTCTGAGGAGCGGGAAAGAGACCCCAAGGTGCTGACATTC
CCAGAATACATCACCAGCTTGTCAGACTCCGGCACCAAGCACATGGCGGCTGGAGTCCGCATGGAGTGCC
ACAGCAAGGGACGATGCCCCTCGTCCTGCCCCCTGTGTCATGTGACATCCAGCCCTGACACCCCTGCTGA
GCCGGTTCTGCTGGAGGTGACCAAAGCAGCCCCCATCTATGAACTAGTGACCAACAACCAGACCCAGAGG
CTCTTGCAGGAGGCTACCATGAGCTCTCTCTGGTGCTCAGGGACTGGAGATGTCATCGAGGACTGGTGTC
GATGTGACTCCACTGCTTTTGGAGCTGATGGACTCCCCACCTGTGCGCCTCTCCCACAGCCTGTGCTGAG
ACTCTCCACGGTTCACGAGCCCAGCAGCACTCTTGTGGTCCTGGAGTGGGAACACTCAGAGCCACCAATC
GGGGTGCAGATTGTAGATTACCTCCTCCGTCAAGAGAAAGTCACTGACAGGATGGACCACTCCAAAGTGG
AGACAGAAACAGTGCTGAGCTTTGTGGACGACATCATCTCTGGAGCAAAGTCTCCTTGTGCAATGCCATC
TCAGGTGCCGGACAAGCAGCTCACCACCATCTCTCTGATCATACGATGCCTGGAACCTGACACCATTTAC
ATGTTCACGCTGTGGGGAGTGGACAACACAGGACGGCGCTCCAGGCCAAGCGACGTGATCGTGAAGACCC
CATGCCCCGTGGTGGATGATGTCAAGGCTCAAGAAATAGCAGACAAGATCTACAATCTCTTCAATGGCTA
CACTAGTGGGAAGGAGCAGCAGACCGCCTACAACACCCTCCTGGATCTGGGTTCCCCCACCTTACACCGG
GTCCTCTACCACTATAACCAGCACTATGAGAGTTTTGGGGAATTCACCTGGCGATGTGAGGATGAGTTAG
GTCCCAGGAAAGCTGGTCTCATCCTTTCCCAGCTTGGGGACCTCAGCAGTTGGTGCAATGGACTCCTTCA
GGAACCCAAGATAAGCTTGCGGCGCAGCTCACTCAAGTACCTGGGGTGCCGCTACAGCGAGATCAAACCC
TACGGACTTGACTGGGCGGAGCTCAGCCGGGACCTCAGGAAGACGTGTGAGGAGCAGACCCTGAGTATCC
CCTACAACGACTATGGGGACAGCAAAGAGATCTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Documentation

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.