Human ASTN1/Astrotactin-1 Protein, ECD2 (Extracellular Domain 2), Biotinylated, Recombinant

ASTN1 (Astrotactin-1) is a two-pass type I transmembrane protein that belongs to the astrotactin family.  ASTN1 contains 2 extracellular domains (ECD), including a 132 aa (amino acid) long small ECD1 and a 900 aa long large ECD2.  The ECD2 is composed of three EGF-like motifs and one Fibronectin type-III motif.  ASTN1 is known to interact with ASTN2 in a calcium-independent manner.  ASTN1 functions as a neuronal adhesion molecule and is required for glial-guided migration of young postmitotic neuroblasts in cortical regions of developing brain, including cerebrum, hippocampus, cerebellum, and olfactory bulb. ASTN1 is also required for normal rate of migration of granule cells during brain development and for normal cerebellum development.

Gene Symbol: ASTN1; Astrotactin-1; ASTN

NCBI Gene ID: 460

Uniprot Entry: O14525

Construct Details: The recombinant human ASTN1 ECD2 protein is expressed as a 705-amino acid protein consisting of the region Val601- Ile1294 of ASTN1 (Uniprot# O14525-2) and a C-terminal His-tag.

Source: Human cells stably expressing ASTN1 ECD2 and growing in chemical-defined media with no animal component or antibiotics

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
VRDCSKDNGG CSKNFRCISD RKLDSTGCVC PSGLSPMKDS SGCYDRHIGV DCSDGFNGGC

        70         80         90        100        110        120
EQLCLQQMAP FPDDPTLYNI LMFCGCIEDY KLGVDGRSCQ LITETCPEGS DCGESRELPM

       130        140        150        160        170        180
NQTLFGEMFF GYNNHSKEVA AGQVLKGTFR QNNFARGLDQ QLPDGLVVAT VPLENQCLEE

       190        200        210        220        230        240
ISEPTPDPDF LTGMVNFSEV SGYPVLQHWK VRSVMYHIKL NQVAISQALS NALHSLDGAT

       250        260        270        280        290        300
SRADFVALLD QFGNHYIQEA IYGFEESCSI WYPNKQVQRR LWLEYEDISK GNSPSDESEE

       310        320        330        340        350        360
RERDPKVLTF PEYITSLSDS GTKHMAAGVR MECHSKGRCP SSCPLCHVTS SPDTPAEPVL

       370        380        390        400        410        420
LEVTKAAPIY ELVTNNQTQR LLQEATMSSL WCSGTGDVIE DWCRCDSTAF GADGLPTCAP

       430        440        450        460        470        480
LPQPVLRLST VHEPSSTLVV LEWEHSEPPI GVQIVDYLLR QEKVTDRMDH SKVETETVLS

       490        500        510        520        530        540
FVDDIISGAK SPCAMPSQVP DKQLTTISLI IRCLEPDTIY MFTLWGVDNT GRRSRPSDVI

       550        560        570        580        590        600
VKTPCPVVDD VKAQEIADKI YNLFNGYTSG KEQQTAYNTL LDLGSPTLHR VLYHYNQHYE

       610        620        630        640        650        660
SFGEFTWRCE DELGPRKAGL ILSQLGDLSS WCNGLLQEPK ISLRRSSLKY LGCRYSEIKP

       670        680        690        700
YGLDWAELSR DLRKTCEEQT LSIPYNDYGD SKEISSGHHH HHHHH

M.W.: Calculated molecular mass 78.8 kDa; estimated by SDS-PAGE under reducing condition: ~80-85 kDa 

Calculated PI: 5.04

Calculated extinction coefficients (M-1 cm-1, at 280nm):  94125

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.