CD228, also known as MELTF and MTf (Melanotransferrin), is a GPI-anchored cell-surface glycoprotein that belongs to the transferrin family. The CD228 gene resides in the same region of chromosome 3 as other members of the transferrin family. CD228 shares sequence similarity and iron-binding properties with other family members. CD228 contains two transferrin-like motifs in its extracellular domain (ECD).  CD228 binds a single atom of iron per subunit and it can also bind zinc.  CD228 may be involved in iron cellular uptake as it seems to be internalized and then recycled back to the cell membrane.  However, the importance of CD228’s iron binding function has not yet been identified. CD228 is also found on melanoma cells.

CD228, also known as MELTF and MTf (Melanotransferrin), is a GPI-anchored cell-surface glycoprotein that belongs to the transferrin family. The CD228 gene resides in the same region of chromosome 3 as other members of the transferrin family. CD228 shares sequence similarity and iron-binding properties with other family members. CD228 contains two transferrin-like motifs in its extracellular domain (ECD).  CD228 binds a single atom of iron per subunit and it can also bind zinc.  CD228 may be involved in iron cellular uptake as it seems to be internalized and then recycled back to the cell membrane.  However, the importance of CD228’s iron binding function has not yet been identified. CD228 is also found on melanoma cells.

 

Product Details

 

Gene Symbol: CD228; MELTF; MTf; MFI2; MTF1; MAP97; Melanotransferrin

 

NCBI Gene ID: 4241

 

Uniprot Entry: P08582

 

Construct Details: Full length human CD228 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

  

Gene Insert Sequence:  

ATGCGGGGTCCGAGCGGGGCTCTGTGGCTGCTCCTGGCTCTGCGCACCGTGCTCGGTGGCATGGAGGTGC
GGTGGTGCGCCACCTCGGACCCAGAGCAGCACAAGTGCGGCAACATGAGCGAGGCCTTCCGGGAAGCGGG
CATCCAGCCCTCCCTCCTCTGCGTCCGGGGCACCTCCGCCGACCACTGCGTCCAGCTCATCGCGGCCCAG
GAGGCTGACGCCATCACTCTGGATGGAGGAGCCATCTATGAGGCGGGAAAGGAGCACGGCCTGAAGCCGG
TGGTGGGCGAAGTGTACGATCAAGAGGTCGGTACCTCCTATTACGCCGTGGCTGTGGTCAGGAGGAGCTC
CCATGTGACCATTGACACCCTGAAAGGCGTGAAGTCCTGCCACACGGGCATCAATCGCACAGTGGGCTGG
AACGTGCCCGTGGGCTACCTGGTGGAGAGCGGCCGCCTCTCGGTGATGGGCTGCGATGTACTCAAAGCTG
TCAGCGACTATTTTGGGGGCAGCTGCGTCCCGGGGGCAGGAGAGACCAGTTACTCTGAGTCCCTCTGTCG
CCTCTGCAGGGGTGACAGCTCTGGGGAAGGGGTGTGTGACAAGAGCCCCCTGGAGAGATACTACGACTAC
AGCGGGGCCTTCCGGTGCCTGGCGGAAGGGGCAGGGGACGTGGCTTTTGTGAAGCACAGCACGGTACTGG
AGAACACGGATGGGAAGACGCTTCCCTCCTGGGGCCAGGCCCTGCTGTCACAGGACTTCGAGCTGCTGTG
CCGGGATGGTAGCCGGGCCGATGTCACCGAGTGGAGGCAGTGCCATCTGGCCCGGGTGCCTGCTCACGCC
GTGGTGGTCCGGGCCGACACAGATGGGGGCCTCATCTTCCGGCTGCTCAACGAAGGCCAGCGTCTGTTCA
GCCACGAGGGCAGCAGCTTCCAGATGTTCAGCTCTGAGGCCTATGGCCAGAAGGATCTACTCTTCAAAGA
CTCTACCTCGGAGCTTGTGCCCATCGCCACACAGACCTATGAGGCGTGGCTGGGCCATGAGTACCTGCAC
GCCATGAAGGGTCTGCTCTGTGACCCCAACCGGCTGCCCCCCTACCTGCGCTGGTGTGTGCTCTCCACTC
CCGAGATCCAGAAGTGTGGAGACATGGCCGTGGCCTTCCGCCGGCAGCGGCTCAAGCCAGAGATCCAGTG
CGTGTCAGCCAAGTCCCCCCAACACTGCATGGAGCGGATCCAGGCTGAGCAGGTCGACGCTGTGACCCTG
AGTGGCGAGGACATTTACACGGCGGGGAAGACGTACGGCCTGGTTCCCGCAGCCGGGGAGCACTATGCCC
CGGAAGACAGCAGCAACTCGTACTACGTGGTGGCCGTGGTGAGACGGGACAGCTCCCACGCCTTCACCTT
GGATGAGCTTCGGGGCAAGCGCTCCTGCCACGCCGGTTTCGGCAGCCCTGCAGGCTGGGATGTCCCCGTG
GGTGCCCTTATTCAGAGAGGCTTCATCCGGCCCAAGGACTGTGACGTCCTCACAGCAGTGAGCGAGTTCT
TCAATGCCAGCTGCGTGCCCGTGAACAACCCCAAGAACTACCCCTCCTCGCTGTGTGCACTGTGCGTGGG
GGACGAGCAGGGCCGCAACAAGTGTGTGGGCAACAGCCAGGAGCGGTATTACGGCTACCGCGGCGCCTTC
AGGTGCCTGGTGGAGAATGCGGGTGACGTTGCCTTCGTCAGGCACACAACCGTCTTTGACAACACAAACG
GCCACAATTCCGAGCCCTGGGCTGCTGAGCTCAGGTCAGAGGACTATGAACTGCTGTGCCCCAACGGGGC
CCGAGCCGAGGTGTCCCAGTTTGCAGCCTGCAACCTGGCACAGATACCACCCCACGCCGTGATGGTCCGG
CCCGACACCAACATCTTCACCGTGTATGGACTGCTGGACAAGGCCCAGGACCTGTTTGGAGACGACCACA
ATAAGAACGGGTTCAAAATGTTCGACTCCTCCAACTATCATGGCCAAGACCTGCTTTTCAAGGATGCCAC
CGTCCGGGCGGTGCCTGTCGGAGAGAAAACCACCTACCGCGGCTGGCTGGGGCTGGACTACGTGGCGGCG
CTGGAAGGGATGTCGTCTCAGCAGTGCTCGGGCGCAGCGGCCCCGGCGCCCGGGGCGCCCCTGCTCCCGC
TGCTGCTGCCCGCCCTCGCCGCCCGCCTGCTCCCGCCCGCCCTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

 

Product datasheet (pdf) and additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.