Human TYRO3/BYK/DTK/RSE/SKY/TIF Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

Tyrosine-protein kinase TYRO3, also known as BYK, DTK, RSE, SKY and TIF, is a single-pass type I transmembrane glycoprotein in the AXL/UFO subfamily of the Receptor Tyrosine Kinase (RTK) family. Like other family members AXL/UFO and MER, TYRO3 contains two immunoglobulin­like (Ig) domains and two fibronectin type III domains in the extracellular domain (ECD) and a cytoplasmic tyrosine kinase domain tail. The AXL/UFO family receptors are involved in regulation of immune responses, cell survival and migration as well as tumorigenesis and metastasis. By binding to its ligand, TULP1 or GAS6, TYRO3 transduces signals from the extracellular matrix into the cytoplasm.  Ligand binding induces dimerization and autophosphorylation of TYRO3 on its cytoplasmic tail that provides docking sites for downstream signaling molecules. Following surface activation, TYRO3 enhances PI3-kinase activity and activates the AKT survival pathway, leading to nuclear translocation and activation of NF-kB. TYRO3 signaling is involved in many biological processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. It also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.  TYRO3 acts as a receptor for various virus, including lassa virus, lymphocytic choriomeningitis virus and ebolavirus

 

 

Gene Symbol: TYRO3; BYK; Dtk; RSE; Rek; Sky; Tif; Etk-2

 

NCBI Gene ID: 7301

 

Uniprot Entry: Q06418

 

Construct Details: Full length human TYRO3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGCGCTGAGGCGGAGCATGGGGCGGCCGGGGCTCCCGCCGCTGCCGCTGCCGCCGCCACCGCGGCTCG
GGCTGCTGCTGGCGGCTCTGGCTTCTCTGCTGCTCCCGGAGTCCGCCGCCGCAGGTCTGAAGCTCATGGG
AGCCCCGGTGAAGCTGACAGTGTCTCAGGGGCAGCCGGTGAAGCTCAACTGCAGTGTGGAGGGGATGGAG
GAGCCTGACATCCAGTGGGTGAAGGATGGGGCTGTGGTCCAGAACTTGGACCAGTTGTACATCCCAGTCA
GCGAGCAGCACTGGATCGGCTTCCTCAGCCTGAAGTCAGTGGAGCGCTCTGACGCCGGCCGGTACTGGTG
CCAGGTGGAGGATGGGGGTGAAACCGAGATCTCCCAGCCAGTGTGGCTCACGGTAGAAGGTGTGCCATTT
TTCACAGTGGAGCCAAAAGATCTGGCAGTGCCACCCAATGCCCCTTTCCAACTGTCTTGTGAGGCTGTGG
GTCCCCCTGAACCTGTTACCATTGTCTGGTGGAGAGGAACTACGAAGATCGGGGGACCCGCTCCCTCTCC
ATCTGTTTTAAATGTAACAGGGGTGACCCAGAGCACCATGTTTTCCTGTGAAGCTCACAACCTAAAAGGC
CTGGCCTCTTCTCGCACAGCCACTGTTCACCTTCAAGCACTGCCTGCAGCCCCCTTCAACATCACCGTGA
CAAAGCTTTCCAGCAGCAACGCTAGTGTGGCCTGGATGCCAGGTGCTGATGGCCGAGCTCTGCTACAGTC
CTGTACAGTTCAGGTGACACAGGCCCCAGGAGGCTGGGAAGTCCTGGCTGTTGTGGTCCCTGTGCCCCCC
TTTACCTGCCTGCTCCGGGACCTGGTGCCTGCCACCAACTACAGCCTCAGGGTGCGCTGTGCCAATGCCT
TGGGGCCCTCTCCCTATGCTGACTGGGTGCCCTTTCAGACCAAGGGTCTAGCCCCAGCCAGCGCTCCCCA
AAACCTCCATGCCATCCGCACAGATTCAGGCCTCATCTTGGAGTGGGAAGAAGTGATCCCCGAGGCCCCT
TTGGAAGGCCCCCTGGGACCCTACAAACTGTCCTGGGTTCAAGACAATGGAACCCAGGATGAGCTGACAG
TGGAGGGGACCAGGGCCAATTTGACAGGCTGGGATCCCCAAAAGGACCTGATCGTACGTGTGTGCGTCTC
CAATGCAGTTGGCTGTGGACCCTGGAGTCAGCCACTGGTGGTCTCTTCTCATGACCGTGCAGGCCAGCAG
GGCCCTCCTCACAGCCGCACATCCTGGGTACCTGTGGTCCTTGGTGTGCTAACGGCCCTGGTGACGGCTG
CTGCCCTGGCCCTCATCCTGCTTCGAAAGAGACGGAAAGAGACGCGGTTTGGGCAAGCCTTTGACAGTGT
CATGGCCCGGGGAGAGCCAGCCGTTCACTTCCGGGCAGCCCGGTCCTTCAATCGAGAAAGGCCCGAGCGC
ATCGAGGCCACATTGGACAGCTTGGGCATCAGCGATGAACTAAAGGAAAAACTGGAGGATGTGCTCATCC
CAGAGCAGCAGTTCACCCTGGGCCGGATGTTGGGCAAAGGAGAGTTTGGTTCAGTGCGGGAGGCCCAGCT
GAAGCAAGAGGATGGCTCCTTTGTGAAAGTGGCTGTGAAGATGCTGAAAGCTGACATCATTGCCTCAAGC
GACATTGAAGAGTTCCTCAGGGAAGCAGCTTGCATGAAGGAGTTTGACCATCCACACGTGGCCAAACTTG
TTGGGGTAAGCCTCCGGAGCAGGGCTAAAGGCCGTCTCCCCATCCCCATGGTCATCTTGCCCTTCATGAA
GCATGGGGACCTGCATGCCTTCCTGCTCGCCTCCCGGATTGGGGAGAACCCCTTTAACCTACCCCTCCAG
ACCCTGATCCGGTTCATGGTGGACATTGCCTGCGGCATGGAGTACCTGAGCTCTCGGAACTTCATCCACC
GAGACCTGGCTGCTCGGAATTGCATGCTGGCAGAGGACATGACAGTGTGTGTGGCTGACTTCGGACTCTC
CCGGAAGATCTACAGTGGGGACTACTATCGTCAAGGCTGTGCCTCCAAACTGCCTGTCAAGTGGCTGGCC
CTGGAGAGCCTGGCCGACAACCTGTATACTGTGCAGAGTGACGTGTGGGCGTTCGGGGTGACCATGTGGG
AGATCATGACACGTGGGCAGACGCCATATGCTGGCATCGAAAACGCTGAGATTTACAACTACCTCATTGG
CGGGAACCGCCTGAAACAGCCTCCGGAGTGTATGGAGGACGTGTATGATCTCATGTACCAGTGCTGGAGT
GCTGACCCCAAGCAGCGCCCGAGCTTTACTTGTCTGCGAATGGAACTGGAGAACATCTTGGGCCAGCTGT
CTGTGCTATCTGCCAGCCAGGACCCCTTATACATCAACATCGAGAGAGCTGAGGAGCCCACTGCGGGAGG
CAGCCTGGAGCTACCTGGCAGGGATCAGCCCTACAGTGGGGCTGGGGATGGCAGTGGCATGGGGGCAGTG
GGTGGCACTCCCAGTGACTGTCGGTACATACTCACCCCCGGAGGGCTGGCTGAGCAGCCAGGGCAGGCAG
AGCACCAGCCAGAGAGTCCCCTCAATGAGACACAGAGGCTTTTGCTGCTGCAGCAAGGGCTACTGCCACA
CAGTAGCTGTTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.