HVEM (herpesvirus entry mediator), also referred to as CD270, TR2 and ATAR, is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF14).  HVEM is widely expressed, with the highest expression in lung, spleen and thymus. It is found in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain (ECD) of HVEM contains three cysteine­rich repeats characteristic of TNF receptor superfamily members. The ECD of HVEM interacts directly with the herpes simplex virus (HSV) envelope glycoprotein D, contributing partly to HSV entry mechanism. The short cytoplasmic region of HVEM lacks a death domain, but contains a TRAF interaction motif for recruiting TRAFs and transducing signaling pathways that activate inflammatory and inhibitory T-cell immune responses. Two TNF superfamily ligands lymphotoxin-α (also known as TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM. Both ligands initiate the positive signaling activities. However, recent studies have also revealed that HVEM may be involved in the inhibitory pathway for T cells through serving as a receptor for BTLA (B and T lymphocyte attenuator) or CD272.

HVEM (herpesvirus entry mediator), also referred to as CD270, TR2 and ATAR, is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF14).  HVEM is widely expressed, with the highest expression in lung, spleen and thymus. It is found in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain (ECD) of HVEM contains three cysteine­rich repeats characteristic of TNF receptor superfamily members. The ECD of HVEM interacts directly with the herpes simplex virus (HSV) envelope glycoprotein D, contributing partly to HSV entry mechanism. The short cytoplasmic region of HVEM lacks a death domain, but contains a TRAF interaction motif for recruiting TRAFs and transducing signaling pathways that activate inflammatory and inhibitory T-cell immune responses. Two TNF superfamily ligands lymphotoxin-α (also known as TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM. Both ligands initiate the positive signaling activities. However, recent studies have also revealed that HVEM may be involved in the inhibitory pathway for T cells through serving as a receptor for BTLA (B and T lymphocyte attenuator) or CD272.

  

Product Details

 

Gene Symbol: TNFRSF14; CD270; TR2; ATAR; HVEA; LIGHTR; UNQ329/PRO509

  

NCBI Gene ID: 8764

 

Uniprot Entry: Q92956

 

Construct Details: Full length human HVEM gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGAGCCTCCTGGAGACTGGGGGCCTCCTCCCTGGAGATCCACCCCCAAAACCGACGTCTTGAGGCTGGTGCTGTATCT

CACCTTCCTGGGAGCCCCCTGCTACGCCCCAGCTCTGCCGTCCTGCAAGGAGGACGAGTACCCAGTGGGCTCCGAGTGCT

GCCCCAAGTGCAGTCCAGGTTATCGTGTGAAGGAGGCCTGCGGGGAGCTGACGGGCACAGTGTGTGAACCCTGCCCTCCA

GGCACCTACATTGCCCACCTCAATGGCCTAAGCAAGTGTCTGCAGTGCCAAATGTGTGACCCAGCCATGGGCCTGCGCGC

GAGCCGGAACTGCTCCAGGACAGAGAACGCCGTGTGTGGCTGCAGCCCAGGCCACTTCTGCATCGTCCAGGACGGGGACC

ACTGCGCCGCGTGCCGCGCTTACGCCACCTCCAGCCCGGGCCAGAGGGTGCAGAAGGGAGGCACCGAGAGTCAGGACACC

CTGTGTCAGAACTGCCCCCCGGGGACCTTCTCTCCCAATGGGACCCTGGAGGAATGTCAGCACCAGACCAAGTGCAGCTG

GCTGGTGACGAAGGCCGGAGCTGGGACCAGCAGCTCCCACTGGGTATGGTGGTTTCTCTCAGGGAGCCTCGTCATCGTCA

TTGTTTGCTCCACAGTTGGCCTAATCATATGTGTGAAAAGAAGAAAGCCAAGGGGTGATGTAGTCAAGGTGATCGTCTCC

GTCCAGCGGAAAAGACAGGAGGCAGAAGGTGAGGCCACAGTCATTGAGGCCCTGCAGGCCCCTCCGGACGTCACCACGGT

GGCCGTGGAGGAGACAATACCCTCATTCACGGGGAGGAGCCCAAACCACTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Cell 87:427 (1996).

2. J. Biol. Chem. 272:13471 (1997). 

3. Immunity 8:21 (1998).

4. Immunol. Rev.202: 49 (2004). 

5. Proc. Natl. Acad. Sci. 102: 13218 (2005). 

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0096-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.