EDA2R (ectodysplasin A2 receptor), also known as XEDAR (X­linked Ectodysplasin Receptor), is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF27). The extracellular domain (ECD) of EDA2R contains three TNFR cysteine­rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. The cytoplasmic region of EDA2R lacks a death domain (DD) that is found in EDAR and other TNFRSF members. EDA2R is widely expressed, notably in embryonic basal epidermal cells and maturing hair follicles. EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Other than a 2 aa deletion in its TNF­like domain, EDA­A2 is identical to EDA­A1. Mutations in EDA give rise to a clinical syndrome hypohidrotic ectodermal dysplasia (HED), which is characterized by loss of hair, sweat glands, and teeth. EDA2R specifically binds to EDA-A2 isoform but not EDA-A1. EDA2R itself is strongly associated with androgenetic alopecia (male hair loss). Even though it does not contain a cytoplasmic death domain, EDA2R can associate with Fas and induce p53-dependent cell death. EDA2R is down-regulated in breast, colon, and lung cancers, particularly in patients with p53 mutations. EDA2R also plays a role in EDA-A2 induced skeletal muscle degeneration and osteoblast differentiation.

EDA2R (ectodysplasin A2 receptor), also known as XEDAR (X­linked Ectodysplasin Receptor), is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF27). The extracellular domain (ECD) of EDA2R contains three TNFR cysteine­rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. The cytoplasmic region of EDA2R lacks a death domain (DD) that is found in EDAR and other TNFRSF members. EDA2R is widely expressed, notably in embryonic basal epidermal cells and maturing hair follicles. EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Other than a 2 aa deletion in its TNF­like domain, EDA­A2 is identical to EDA­A1. Mutations in EDA give rise to a clinical syndrome hypohidrotic ectodermal dysplasia (HED), which is characterized by loss of hair, sweat glands, and teeth. EDA2R specifically binds to EDA-A2 isoform but not EDA-A1. EDA2R itself is strongly associated with androgenetic alopecia (male hair loss). Even though it does not contain a cytoplasmic death domain, EDA2R can associate with Fas and induce p53-dependent cell death. EDA2R is down-regulated in breast, colon, and lung cancers, particularly in patients with p53 mutations. EDA2R also plays a role in EDA-A2 induced skeletal muscle degeneration and osteoblast differentiation.

  

Product Details

 

Gene Symbol: XEDAR; EDAA2R; EDA-A2R; TNFRSF27; UNQ2448/PRO5727/PRO34080

  

NCBI Gene ID: 60401

 

Uniprot Entry: Q9HAV5

 

Construct Details: Full length human EDA2R gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGATTGCCAAGAAAATGAGTACTGGGACCAATGGGGACGGTGTGTCACCTGCCAACGGTGTGGTCCTGGACAGGAGCT

ATCCAAGGATTGTGGTTATGGAGAGGGTGGAGATGCCTACTGCACAGCCTGCCCTCCTCGCAGGTACAAAAGCAGCTGGG

GCCACCACAGATGTCAGAGTTGCATCACCTGTGCTGTCATCAATCGTGTTCAGAAGGTCAACTGCACAGCTACCTCTAAT

GCTGTCTGTGGGGACTGTTTGCCCAGGTTCTACCGAAAGACACGCATTGGAGGCCTGCAGGACCAAGAGTGCATCCCGTG

CACGAAGCAGACCCCCACCTCTGAGGTTCAATGTGCCTTCCAGTTGAGCTTAGTGGAGGCAGATACACCCACAGTGCCCC

CTCAGGAGGCCACACTTGTTGCACTGGTGAGCAGCCTGCTAGTGGTGTTTACCCTGGCCTTCCTGGGGCTCTTCTTCCTC

TACTGCAAGCAGTTCTTCAACAGACATTGCCAGCGTGGAGGTTTGCTGCAGTTTGAGGCTGATAAAACAGCAAAGGAGGA

ATCTCTCTTCCCCGTGCCACCCAGCAAGGAGACCAGTGCTGAGTCCCAAGTGAGTGAGAACATCTTTCAGACCCAGCCAC

TTAACCCTATCCTCGAGGACGACTGCAGCTCGACTAGTGGCTTCCCCACACAGGAGTCCTTTACCATGGCCTCCTGCACC

TCAGAGAGCCACTCCCACTGGGTCCACAGCCCCATCGAATGCACAGAGCTGGACCTGCAAAAGTTTTCCAGCTCTGCCTC

CTATACTGGAGCTGAGACCTTGGGGGGAAACACAGTCGAAAGCACTGGAGACAGGCTGGAGCTCAATGTGCCCTTTGAAG

TTCCCAGCCCTTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Science 290:523 (2000).

2. J. Biol. Chem. 277:44953 (2002).

3. Mol. Cell. Biol. 24:1608 (2004).

4. Oncogene 28:3081 (2009).

5. Clin. Cancer Res. 16:1140 (2010). 

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0301-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.