The mannose 6-phophate receptors (MPRs) are single-pass type I transmembrane glycoproteins that target the newly synthesized hydrolases from the Golgi complex and the cell surface to lysosomes. There are two different MPRs, one of ~300kDa termed CI-MPR (cation-independent) and a smaller, dimeric receptor of ~46kDa termed CD-MPR (cation-depnendent). Both MPRss are P-type lectins that bind terminal mannose 6-phosphate (M6P) with similar affinity (CI-MPR = 7 μM, CD-MPR = 8 μM) and contain similar cytoplasmic signals for intracellular trafficking. CD-MPR shows greatly enhanced binding to M6P in the presence of divalent cations, such as manganese. The MPRs, when bound to their cargo via M6P, are recognized by the GGA family of clathrin adaptor proteins and accumulate in forming clathrin-coated vesicles. The vesicles are trafficked to the early endosome where, in the relatively low pH environment of the endosome, the MPRs release their cargo. The cargo proteins are then trafficked to the lysosome via the late endosome independently of the MPRs. CD-MPR is also known as M6PR that contain a 157 amino-acid M6P-binding-domain in the extracellular/luminal region. CI-MPR was also identified as the cell surface receptor for insulin-like growth factor 2 (IGF2R/CD222). IGF2R consists of 15 repeats homologous to the M6P-binding domain present in M6PR/CD-MPR and two of these 15 repeats are responsible for binding to M6P. 

The mannose 6-phophate receptors (MPRs) are single-pass type I transmembrane glycoproteins that target the newly synthesized hydrolases from the Golgi complex and the cell surface to lysosomes. There are two different MPRs, one of ~300kDa termed CI-MPR (cation-independent) and a smaller, dimeric receptor of ~46kDa termed CD-MPR (cation-depnendent). Both MPRss are P-type lectins that bind terminal mannose 6-phosphate (M6P) with similar affinity (CI-MPR = 7 μM, CD-MPR = 8 μM) and contain similar cytoplasmic signals for intracellular trafficking. CD-MPR shows greatly enhanced binding to M6P in the presence of divalent cations, such as manganese. The MPRs, when bound to their cargo via M6P, are recognized by the GGA family of clathrin adaptor proteins and accumulate in forming clathrin-coated vesicles. The vesicles are trafficked to the early endosome where, in the relatively low pH environment of the endosome, the MPRs release their cargo. The cargo proteins are then trafficked to the lysosome via the late endosome independently of the MPRs. CD-MPR is also known as M6PR that contain a 157 amino-acid M6P-binding-domain in the extracellular/luminal region. CI-MPR was also identified as the cell surface receptor for insulin-like growth factor 2 (IGF2R/CD222). IGF2R consists of 15 repeats homologous to the M6P-binding domain present in M6PR/CD-MPR and two of these 15 repeats are responsible for binding to M6P. 

 

Product Details

 

Gene Symbol: M6PR; SMPR; MPR46; CD-MPR; MPR 46; MPR-46; MPRD

 

NCBI Gene ID: 4074

 

Uniprot Entry: P20645

 

Construct Details: Full length human M6PR gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGTTCCCTTTCTACAGCTGCTGGAGGACTGGACTGCTACTACTACTCCTGGCTGTGGCAGTGAGAGAATCCTGGCAGAC

AGAAGAAAAAACTTGCGACTTGGTAGGAGAAAAGGGTAAAGAGTCAGAGAAAGAGTTGGCTCTAGTGAAGAGGCTGAAAC

CACTGTTTAATAAAAGCTTTGAGAGCACTGTGGGCCAGGGTTCAGACACATACATCTACATCTTCAGGGTGTGCCGGGAA

GCTGGCAACCACACTTCTGGGGCAGGCCTGGTGCAAATCAACAAAAGTAATGGGAAGGAGACAGTGGTAGGGAGACTCAA

CGAGACTCACATCTTCAACGGAAGTAATTGGATCATGCTGATCTATAAAGGGGGTGATGAATATGACAACCACTGTGGCA

AGGAGCAGCGTCGTGCAGTGGTGATGATCTCCTGCAATCGACACACCCTAGCGGACAATTTTAACCCTGTGTCTGAGGAG

CGTGGCAAAGTCCAAGATTGTTTCTACCTCTTTGAGATGGATAGCAGCCTGGCCTGTTCACCAGAGATCTCCCACCTCAG

TGTGGGTTCCATCTTACTTGTCACGTTTGCATCACTGGTTGCTGTTTATGTTGTTGGGGGGTTCCTATACCAGCGACTGG

TAGTGGGAGCCAAAGGAATGGAGCAGTTTCCCCACTTAGCCTTCTGGCAGGATCTTGGCAACCTGGTAGCAGATGGCTGT

GACTTTGTCTGCCGTTCTAAACCTCGAAATGTGCCTGCAGCATATCGTGGTGTGGGGGATGACCAGCTGGGGGAGGAGTC

AGAAGAAAGGGATGACCATTTATTACCAATGTAG

  

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 82: 4428 (1985).

2. J. Biol. Chem. 260: 12008 (1985).

3. Proc. Natl. Acad. Sci. 84:5575 (1987). 

4. J. Biol. Chem. 264: 7970 (1989).

5. Science 292:1712 (2001).

6. J. Biol. Chem. 277:18477 (2002). 

 

 

 

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0131-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.