TACI (transmembrane activator and CAML­interactor), also known as CD267, is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF13B). The TNFRSF members have crucial roles in both innate and adaptive immunity as well as in apoptosis. The extracellular region of TACI contains two TNFR-cysteine rich repeats that mediate interactions with its TNF family ligands, including APRIL/TNFSF13 and BAFF/TNFSF13B. TACI binds both ligands with similar high affinity.  Within the TNFRSF, it shares the highest homology with BCMA/TNFRSF17. TACI and BCMA can bind both APRIL and BAFF. TACI is expressed on the cell surface of B cells and activated T cells, but not on resting T cells. TACI may play an important role in B cell development, function and regulation. TACI interacts with calcium-modulator and cyclophilin ligand (CAML). TACI mediates calcineurin-dependent activation of NF-AT, NF-κB and AP-1.  TACI is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.  Mutations in the TACI gene are associated with common variable immunodeficiency 2 (CVID2) and immunoglobulin A deficiency 2 (IGAD2) in humans.

TACI (transmembrane activator and CAML­interactor), also known as CD267, is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF13B). The TNFRSF members have crucial roles in both innate and adaptive immunity as well as in apoptosis. The extracellular region of TACI contains two TNFR-cysteine rich repeats that mediate interactions with its TNF family ligands, including APRIL/TNFSF13 and BAFF/TNFSF13B. TACI binds both ligands with similar high affinity.  Within the TNFRSF, it shares the highest homology with BCMA/TNFRSF17. TACI and BCMA can bind both APRIL and BAFF. TACI is expressed on the cell surface of B cells and activated T cells, but not on resting T cells. TACI may play an important role in B cell development, function and regulation. TACI interacts with calcium-modulator and cyclophilin ligand (CAML). TACI mediates calcineurin-dependent activation of NF-AT, NF-κB and AP-1.  TACI is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.  Mutations in the TACI gene are associated with common variable immunodeficiency 2 (CVID2) and immunoglobulin A deficiency 2 (IGAD2) in humans.

  

Product Details

 

Gene Symbol: TACI; CD267; CVID; RYZN; CVID2; TNFRSF13B

 

NCBI Gene ID: 23495

 

Uniprot Entry: O14836

 

Construct Details: The recombinant human TACI-Fc fusion is expressed as a 391 amino acid protein consisting of Ser2 - Ser165 region of TACI (UniProt accession #O14836) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

   

Source: Human cells stably expressing human TACI-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

SGLGRSRRGG RSRVDQEERF PQGLWTGVAM RSCPEEQYWD PLLGTCMSCK TICNHQSQRT 

        70         80         90        100        110        120 

CAAFCRSLSC RKEQGKFYDH LLRDCISCAS ICGQHPKQCA YFCENKLRSP VNLPPELRRQ 

       130        140        150        160        170        180 

RSGEVENNSD NSGRYQGLEH RGSEASPALP GLKLSADQVA LVYSTGTHTC PPCPAPELLG 

       190        200        210        220        230        240 

GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY 

       250        260        270        280        290        300 

NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE 

       310        320        330        340        350        360 

EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR 

       370        380        390 

WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K 

 

M.W.: Calculated molecular mass (kDa): 43.9; Estimated by SDS-PAGE under reducing condition (kDa): 45-50

 

Calculated PI: 8.45

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 54985

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand BAFF.  Inhibits  BAFF-mediated signaling activities including NF-κB activation and proliferation of anti­-IgM co-stimulated B cells.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Science, 278:138 (1997). 

2. J. Exp. Med. 192:137 (2000).

3. Nature 404:995 (2000). 

4. Nature Immunol.1:37 (2000).

5. Nat Genet. 37: 820 (2005)

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0225-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.