Human TLR1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
TLR1 is a single-pass type I transmembrane glycoprotein belonging to the Toll-like receptor (TLR) family. The TLR family members play a fundamental role in pathogen recognition and activation of innate immunity. They serve as pattern recognition receptors for microbial pathogens. There are at least 11 mouse and 10 human TLR members. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs contain a large number of leucinerich repeats (LRRs) and a cytoplasmic tail with a Toll/IL1 receptor (TIR) domain. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR1 contains 19 LRR repeats and 1 LRRCT in the extracellular region. The various TLRs exhibit different patterns of expression. TLR1 is ubiquitously expressed, and at higher levels than other TLRs. It is found on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligandindependent association with TLR2. TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols.
Gene Symbol: TLR1; TIL; CD281; rsc786; TIL. LPRS5; KIAA0012
NCBI Gene ID: 7096
Uniprot Entry: Q15399
Construct Details: The recombinant human TLR1-Fc fusion protein is expressed as a 778 amino acid protein consisting of Ser25 Glu574 region of TLR1 (UniProt accession #Q15399) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image above).
Source: Human cells stably expressing human TLR1-Fc and growing in chemical-defined media with no animal components or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
SEFLVDRSKN GLIHVPKDLS QKTTILNISQ NYISELWTSD ILSLSKLRIL IISHNRIQYL
70 80 90 100 110 120
DISVFKFNQE LEYLDLSHNK LVKISCHPTV NLKHLDLSFN AFDALPICKE FGNMSQLKFL
130 140 150 160 170 180
GLSTTHLEKS SVLPIAHLNI SKVLLVLGET YGEKEDPEGL QDFNTESLHI VFPTNKEFHF
190 200 210 220 230 240
ILDVSVKTVA NLELSNIKCV LEDNKCSYFL SILAKLQTNP KLSNLTLNNI ETTWNSFIRI
250 260 270 280 290 300
LQLVWHTTVW YFSISNVKLQ GQLDFRDFDY SGTSLKALSI HQVVSDVFGF PQSYIYEIFS
310 320 330 340 350 360
NMNIKNFTVS GTRMVHMLCP SKISPFLHLD FSNNLLTDTV FENCGHLTEL ETLILQMNQL
370 380 390 400 410 420
KELSKIAEMT TQMKSLQQLD ISQNSVSYDE KKGDCSWTKS LLSLNMSSNI LTDTIFRCLP
430 440 450 460 470 480
PRIKVLDLHS NKIKSIPKQV VKLEALQELN VAFNSLTDLP GCGSFSSLSV LIIDHNSVSH
490 500 510 520 530 540
PSADFFQSCQ KMRSIKAGDN PFQCTCELGE FVKNIDQVSS EVLEGWPDSY KCDYPESYRG
550 560 570 580 590 600
TLLKDFHMSE STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE
610 620 630 640 650 660
DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP
670 680 690 700 710 720
APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN
730 740 750 760 770
NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W.: Calculated molecular mass (kDa): 88.1; Estimated by SDS-PAGE under reducing condition (kDa): 90-100
Calculated PI: 6.16
Calculated extinction coefficients (M-1 cm-1, at 280nm): 88905
Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity: Binds to the triacylated peptide biotinylated Pam3CysSerLys4 (PAM3CSK4).
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
1. Proc. Natl. Acad. Sci. 95:588 (1998).
2. Proc. Natl. Acad. Sci. 97:13766 (2000).
3. J. Biol. Chem. 279:16971 (2004).
4. J. Immunol. 174:1566 (2005).
5. J. Biol. Chem. 280:8606. 13 (2005).
6. Semin. Immunol. 19:3 (2007).
Product datasheet (pdf) can be downloaded here: FCL00J4-PDS.pdf
Additional supporting documents, including COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.